Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12275 and BWI76_RS12280 are separated by 3 nucleotides BWI76_RS12280 and BWI76_RS12285 are separated by 1 nucleotides BWI76_RS12285 and BWI76_RS12290 are separated by 1 nucleotides BWI76_RS12290 and BWI76_RS12295 overlap by 4 nucleotides BWI76_RS12295 and BWI76_RS12300 are separated by 22 nucleotides
BWI76_RS12275: BWI76_RS12275 - hypothetical protein, at 2,565,648 to 2,565,857
_RS12275
BWI76_RS12280: BWI76_RS12280 - hypothetical protein, at 2,565,861 to 2,566,271
_RS12280
BWI76_RS12285: BWI76_RS12285 - hypothetical protein, at 2,566,273 to 2,566,656
_RS12285
BWI76_RS12290: BWI76_RS12290 - hypothetical protein, at 2,566,658 to 2,567,209
_RS12290
BWI76_RS12295: BWI76_RS12295 - hypothetical protein, at 2,567,206 to 2,567,598
_RS12295
BWI76_RS12300: BWI76_RS12300 - hypothetical protein, at 2,567,621 to 2,568,553
_RS12300
Position (kb)
2566
2567
2568 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2565.724 kb on + strand, within BWI76_RS12275 at 2565.732 kb on - strand, within BWI76_RS12275 at 2565.801 kb on - strand, within BWI76_RS12275 at 2565.805 kb on + strand, within BWI76_RS12275 at 2565.813 kb on - strand, within BWI76_RS12275 at 2565.816 kb on - strand, within BWI76_RS12275 at 2565.917 kb on - strand, within BWI76_RS12280 at 2565.970 kb on + strand, within BWI76_RS12280 at 2565.978 kb on - strand, within BWI76_RS12280 at 2565.992 kb on + strand, within BWI76_RS12280 at 2566.222 kb on - strand, within BWI76_RS12280 at 2566.356 kb on + strand, within BWI76_RS12285 at 2566.435 kb on + strand, within BWI76_RS12285 at 2566.641 kb on + strand at 2566.713 kb on + strand at 2566.768 kb on + strand, within BWI76_RS12290 at 2566.810 kb on - strand, within BWI76_RS12290 at 2566.941 kb on + strand, within BWI76_RS12290 at 2566.943 kb on + strand, within BWI76_RS12290 at 2567.027 kb on - strand, within BWI76_RS12290 at 2567.027 kb on - strand, within BWI76_RS12290 at 2567.121 kb on - strand, within BWI76_RS12290 at 2567.164 kb on + strand at 2567.198 kb on + strand at 2567.430 kb on + strand, within BWI76_RS12295 at 2567.470 kb on - strand, within BWI76_RS12295 at 2567.476 kb on + strand, within BWI76_RS12295 at 2567.501 kb on + strand, within BWI76_RS12295 at 2567.674 kb on - strand at 2567.724 kb on + strand, within BWI76_RS12300 at 2567.724 kb on + strand, within BWI76_RS12300 at 2567.732 kb on - strand, within BWI76_RS12300 at 2567.788 kb on - strand, within BWI76_RS12300 at 2567.953 kb on + strand, within BWI76_RS12300 at 2568.147 kb on + strand, within BWI76_RS12300 at 2568.147 kb on - strand, within BWI76_RS12300 at 2568.162 kb on + strand, within BWI76_RS12300
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,565,724 + BWI76_RS12275 0.36 -2.4 2,565,732 - BWI76_RS12275 0.40 -0.8 2,565,801 - BWI76_RS12275 0.73 -0.4 2,565,805 + BWI76_RS12275 0.75 -0.2 2,565,813 - BWI76_RS12275 0.79 -1.0 2,565,816 - BWI76_RS12275 0.80 -0.4 2,565,917 - BWI76_RS12280 0.14 -0.4 2,565,970 + BWI76_RS12280 0.27 -0.8 2,565,978 - BWI76_RS12280 0.28 -0.8 2,565,992 + BWI76_RS12280 0.32 +0.3 2,566,222 - BWI76_RS12280 0.88 +0.2 2,566,356 + BWI76_RS12285 0.22 -1.8 2,566,435 + BWI76_RS12285 0.42 -0.6 2,566,641 + +0.1 2,566,713 + -1.3 2,566,768 + BWI76_RS12290 0.20 -1.0 2,566,810 - BWI76_RS12290 0.28 -0.8 2,566,941 + BWI76_RS12290 0.51 -1.4 2,566,943 + BWI76_RS12290 0.52 -1.9 2,567,027 - BWI76_RS12290 0.67 -0.9 2,567,027 - BWI76_RS12290 0.67 -0.3 2,567,121 - BWI76_RS12290 0.84 -1.7 2,567,164 + -1.3 2,567,198 + +0.2 2,567,430 + BWI76_RS12295 0.57 -0.4 2,567,470 - BWI76_RS12295 0.67 -0.9 2,567,476 + BWI76_RS12295 0.69 -0.5 2,567,501 + BWI76_RS12295 0.75 -0.2 2,567,674 - -1.0 2,567,724 + BWI76_RS12300 0.11 -1.0 2,567,724 + BWI76_RS12300 0.11 -0.3 2,567,732 - BWI76_RS12300 0.12 -0.4 2,567,788 - BWI76_RS12300 0.18 -0.5 2,567,953 + BWI76_RS12300 0.36 -0.1 2,568,147 + BWI76_RS12300 0.56 -1.7 2,568,147 - BWI76_RS12300 0.56 -4.6 2,568,162 + BWI76_RS12300 0.58 -0.2
Or see this region's nucleotide sequence