Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12155 and BWI76_RS12160 are separated by 57 nucleotides BWI76_RS12160 and BWI76_RS12165 overlap by 1 nucleotides BWI76_RS12165 and BWI76_RS12170 are separated by 2 nucleotides BWI76_RS12170 and BWI76_RS12175 are separated by 130 nucleotides
BWI76_RS12155: BWI76_RS12155 - hypothetical protein, at 2,548,628 to 2,548,849
_RS12155
BWI76_RS12160: BWI76_RS12160 - hypothetical protein, at 2,548,907 to 2,549,506
_RS12160
BWI76_RS12165: BWI76_RS12165 - hypothetical protein, at 2,549,506 to 2,549,712
_RS12165
BWI76_RS12170: BWI76_RS12170 - hypothetical protein, at 2,549,715 to 2,550,011
_RS12170
BWI76_RS12175: BWI76_RS12175 - hypothetical protein, at 2,550,142 to 2,550,825
_RS12175
Position (kb)
2549
2550
2551 Strain fitness (log2 ratio)
-2
-1
0
1 at 2548.801 kb on + strand, within BWI76_RS12155 at 2548.801 kb on + strand, within BWI76_RS12155 at 2548.878 kb on + strand at 2548.890 kb on + strand at 2548.898 kb on + strand at 2548.925 kb on - strand at 2548.934 kb on - strand at 2548.944 kb on - strand at 2548.956 kb on + strand at 2548.969 kb on - strand, within BWI76_RS12160 at 2548.969 kb on - strand, within BWI76_RS12160 at 2548.970 kb on - strand, within BWI76_RS12160 at 2549.023 kb on + strand, within BWI76_RS12160 at 2549.023 kb on + strand, within BWI76_RS12160 at 2549.023 kb on + strand, within BWI76_RS12160 at 2549.031 kb on - strand, within BWI76_RS12160 at 2549.133 kb on + strand, within BWI76_RS12160 at 2549.134 kb on + strand, within BWI76_RS12160 at 2549.313 kb on - strand, within BWI76_RS12160 at 2549.452 kb on - strand at 2549.937 kb on - strand, within BWI76_RS12170 at 2549.963 kb on + strand, within BWI76_RS12170 at 2549.989 kb on + strand at 2550.030 kb on + strand at 2550.058 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,548,801 + BWI76_RS12155 0.78 +0.2 2,548,801 + BWI76_RS12155 0.78 +0.0 2,548,878 + +0.2 2,548,890 + +1.2 2,548,898 + -0.9 2,548,925 - -0.5 2,548,934 - +0.9 2,548,944 - +0.4 2,548,956 + -0.4 2,548,969 - BWI76_RS12160 0.10 -1.0 2,548,969 - BWI76_RS12160 0.10 -1.5 2,548,970 - BWI76_RS12160 0.10 +0.0 2,549,023 + BWI76_RS12160 0.19 -2.4 2,549,023 + BWI76_RS12160 0.19 +1.0 2,549,023 + BWI76_RS12160 0.19 -0.4 2,549,031 - BWI76_RS12160 0.21 -2.3 2,549,133 + BWI76_RS12160 0.38 -1.3 2,549,134 + BWI76_RS12160 0.38 +1.2 2,549,313 - BWI76_RS12160 0.68 -0.1 2,549,452 - -1.4 2,549,937 - BWI76_RS12170 0.75 +0.4 2,549,963 + BWI76_RS12170 0.84 -1.5 2,549,989 + -1.8 2,550,030 + -0.4 2,550,058 - +0.8
Or see this region's nucleotide sequence