Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08930 and BWI76_RS08935 are separated by 285 nucleotides BWI76_RS08935 and BWI76_RS08940 are separated by 68 nucleotides BWI76_RS08940 and BWI76_RS08945 are separated by 45 nucleotides
BWI76_RS08930: BWI76_RS08930 - hypothetical protein, at 1,881,754 to 1,882,014
_RS08930
BWI76_RS08935: BWI76_RS08935 - hypothetical protein, at 1,882,300 to 1,882,566
_RS08935
BWI76_RS08940: BWI76_RS08940 - PKHD-type hydroxylase, at 1,882,635 to 1,883,312
_RS08940
BWI76_RS08945: BWI76_RS08945 - catecholate siderophore receptor Fiu, at 1,883,358 to 1,885,646
_RS08945
Position (kb) 1882
1883
1884 Strain fitness (log2 ratio) -2
-1
0
1 at 1881.706 kb on + strand at 1881.727 kb on - strand at 1881.768 kb on + strand at 1882.032 kb on - strand at 1882.034 kb on + strand at 1882.034 kb on + strand at 1882.211 kb on + strand at 1882.571 kb on - strand at 1882.576 kb on + strand at 1882.589 kb on + strand at 1882.591 kb on + strand at 1882.661 kb on + strand at 1882.839 kb on + strand, within BWI76_RS08940 at 1882.898 kb on + strand, within BWI76_RS08940 at 1883.066 kb on - strand, within BWI76_RS08940 at 1883.139 kb on + strand, within BWI76_RS08940 at 1883.152 kb on + strand, within BWI76_RS08940 at 1883.177 kb on - strand, within BWI76_RS08940 at 1883.457 kb on - strand at 1883.539 kb on + strand at 1883.685 kb on + strand, within BWI76_RS08945 at 1883.730 kb on + strand, within BWI76_RS08945 at 1883.954 kb on + strand, within BWI76_RS08945
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,881,706 + +0.1 1,881,727 - -1.1 1,881,768 + -2.0 1,882,032 - +0.4 1,882,034 + -1.8 1,882,034 + -0.7 1,882,211 + +1.1 1,882,571 - -0.2 1,882,576 + -0.6 1,882,589 + +1.5 1,882,591 + +0.5 1,882,661 + +1.0 1,882,839 + BWI76_RS08940 0.30 -0.7 1,882,898 + BWI76_RS08940 0.39 +0.1 1,883,066 - BWI76_RS08940 0.64 +0.3 1,883,139 + BWI76_RS08940 0.74 -0.3 1,883,152 + BWI76_RS08940 0.76 -1.6 1,883,177 - BWI76_RS08940 0.80 -0.9 1,883,457 - -2.3 1,883,539 + +0.5 1,883,685 + BWI76_RS08945 0.14 +0.5 1,883,730 + BWI76_RS08945 0.16 +0.4 1,883,954 + BWI76_RS08945 0.26 +0.5
Or see this region's nucleotide sequence