Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06500 and BWI76_RS06505 are separated by 45 nucleotides BWI76_RS06505 and BWI76_RS06510 are separated by 303 nucleotides BWI76_RS06510 and BWI76_RS06515 overlap by 41 nucleotides BWI76_RS06515 and BWI76_RS06520 are separated by 207 nucleotides
BWI76_RS06500: BWI76_RS06500 - muropeptide transporter AmpG, at 1,365,804 to 1,367,279
_RS06500
BWI76_RS06505: BWI76_RS06505 - hypothetical protein, at 1,367,325 to 1,367,903
_RS06505
BWI76_RS06510: BWI76_RS06510 - BolA family transcriptional regulator, at 1,368,207 to 1,368,524
_RS06510
BWI76_RS06515: BWI76_RS06515 - hypothetical protein, at 1,368,484 to 1,368,664
_RS06515
BWI76_RS06520: BWI76_RS06520 - trigger factor, at 1,368,872 to 1,370,170
_RS06520
Position (kb) 1368
1369 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1367.394 kb on - strand, within BWI76_RS06505 at 1367.722 kb on + strand, within BWI76_RS06505 at 1367.825 kb on + strand, within BWI76_RS06505 at 1367.840 kb on + strand, within BWI76_RS06505 at 1367.860 kb on + strand at 1368.159 kb on + strand at 1368.214 kb on + strand at 1368.244 kb on - strand, within BWI76_RS06510 at 1368.276 kb on + strand, within BWI76_RS06510 at 1368.416 kb on - strand at 1368.456 kb on - strand, within BWI76_RS06510 at 1368.458 kb on - strand, within BWI76_RS06510 at 1368.458 kb on - strand, within BWI76_RS06510 at 1368.507 kb on + strand at 1368.515 kb on - strand at 1368.807 kb on - strand at 1368.967 kb on + strand at 1369.034 kb on + strand, within BWI76_RS06520 at 1369.227 kb on + strand, within BWI76_RS06520 at 1369.227 kb on + strand, within BWI76_RS06520 at 1369.280 kb on + strand, within BWI76_RS06520 at 1369.328 kb on + strand, within BWI76_RS06520
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,367,394 - BWI76_RS06505 0.12 +0.7 1,367,722 + BWI76_RS06505 0.69 +0.9 1,367,825 + BWI76_RS06505 0.86 +0.5 1,367,840 + BWI76_RS06505 0.89 +1.0 1,367,860 + +0.2 1,368,159 + +0.7 1,368,214 + +0.2 1,368,244 - BWI76_RS06510 0.12 -1.7 1,368,276 + BWI76_RS06510 0.22 -0.9 1,368,416 - +1.2 1,368,456 - BWI76_RS06510 0.78 -2.9 1,368,458 - BWI76_RS06510 0.79 -0.5 1,368,458 - BWI76_RS06510 0.79 -0.7 1,368,507 + +0.8 1,368,515 - +0.7 1,368,807 - -2.8 1,368,967 + -2.2 1,369,034 + BWI76_RS06520 0.12 -2.3 1,369,227 + BWI76_RS06520 0.27 -1.2 1,369,227 + BWI76_RS06520 0.27 -2.6 1,369,280 + BWI76_RS06520 0.31 -1.5 1,369,328 + BWI76_RS06520 0.35 +0.9
Or see this region's nucleotide sequence