Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02575 and BWI76_RS02580 are separated by 85 nucleotides BWI76_RS02580 and BWI76_RS02585 are separated by 45 nucleotides BWI76_RS02585 and BWI76_RS02590 are separated by 105 nucleotides
BWI76_RS02575: BWI76_RS02575 - exoribonuclease R, at 526,736 to 529,183
_RS02575
BWI76_RS02580: BWI76_RS02580 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase, at 529,269 to 530,000
_RS02580
BWI76_RS02585: BWI76_RS02585 - hypothetical protein, at 530,046 to 530,399
_RS02585
BWI76_RS02590: BWI76_RS02590 - isovaleryl-CoA dehydrogenase, at 530,505 to 532,142
_RS02590
Position (kb) 530
531 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 529.089 kb on - strand at 529.149 kb on - strand at 529.667 kb on + strand, within BWI76_RS02580 at 529.792 kb on + strand, within BWI76_RS02580 at 529.804 kb on + strand, within BWI76_RS02580 at 529.971 kb on - strand at 530.042 kb on - strand at 530.042 kb on - strand at 530.114 kb on - strand, within BWI76_RS02585 at 530.114 kb on - strand, within BWI76_RS02585 at 530.253 kb on + strand, within BWI76_RS02585 at 530.286 kb on - strand, within BWI76_RS02585 at 530.351 kb on + strand, within BWI76_RS02585 at 530.353 kb on + strand, within BWI76_RS02585 at 530.526 kb on - strand at 530.552 kb on - strand at 530.827 kb on + strand, within BWI76_RS02590 at 530.931 kb on - strand, within BWI76_RS02590 at 531.256 kb on - strand, within BWI76_RS02590 at 531.261 kb on + strand, within BWI76_RS02590 at 531.264 kb on - strand, within BWI76_RS02590 at 531.351 kb on + strand, within BWI76_RS02590
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 529,089 - +1.0 529,149 - -1.3 529,667 + BWI76_RS02580 0.54 +1.0 529,792 + BWI76_RS02580 0.71 +3.0 529,804 + BWI76_RS02580 0.73 +1.3 529,971 - -0.6 530,042 - +0.5 530,042 - +0.3 530,114 - BWI76_RS02585 0.19 +0.1 530,114 - BWI76_RS02585 0.19 +0.6 530,253 + BWI76_RS02585 0.58 +0.5 530,286 - BWI76_RS02585 0.68 -0.0 530,351 + BWI76_RS02585 0.86 +0.6 530,353 + BWI76_RS02585 0.87 +1.0 530,526 - -0.0 530,552 - -0.4 530,827 + BWI76_RS02590 0.20 -0.1 530,931 - BWI76_RS02590 0.26 -1.8 531,256 - BWI76_RS02590 0.46 +1.2 531,261 + BWI76_RS02590 0.46 -0.0 531,264 - BWI76_RS02590 0.46 -0.0 531,351 + BWI76_RS02590 0.52 +0.2
Or see this region's nucleotide sequence