Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02545 and BWI76_RS02550 are separated by 2 nucleotides BWI76_RS02550 and BWI76_RS02555 are separated by 63 nucleotides BWI76_RS02555 and BWI76_RS02560 are separated by 218 nucleotides BWI76_RS02560 and BWI76_RS02565 are separated by 102 nucleotides
BWI76_RS02545: BWI76_RS02545 - protease modulator HflK, at 521,687 to 522,943
_RS02545
BWI76_RS02550: BWI76_RS02550 - protease modulator HflC, at 522,946 to 523,950
_RS02550
BWI76_RS02555: BWI76_RS02555 - hypothetical protein, at 524,014 to 524,304
_RS02555
BWI76_RS02560: BWI76_RS02560 - membrane protein, at 524,523 to 524,720
_RS02560
BWI76_RS02565: BWI76_RS02565 - adenylosuccinate synthase, at 524,823 to 526,121
_RS02565
Position (kb) 522
523
524 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 522.172 kb on - strand, within BWI76_RS02545 at 522.186 kb on - strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.383 kb on - strand, within BWI76_RS02545 at 522.576 kb on - strand, within BWI76_RS02545 at 522.592 kb on + strand, within BWI76_RS02545 at 522.628 kb on + strand, within BWI76_RS02545 at 522.628 kb on + strand, within BWI76_RS02545 at 522.712 kb on + strand, within BWI76_RS02545 at 522.898 kb on - strand at 523.054 kb on + strand, within BWI76_RS02550 at 523.078 kb on + strand, within BWI76_RS02550 at 523.078 kb on + strand, within BWI76_RS02550 at 523.359 kb on - strand, within BWI76_RS02550 at 523.388 kb on + strand, within BWI76_RS02550 at 523.677 kb on - strand, within BWI76_RS02550 at 523.828 kb on + strand, within BWI76_RS02550 at 523.874 kb on + strand at 523.946 kb on + strand at 524.042 kb on + strand at 524.048 kb on + strand, within BWI76_RS02555 at 524.067 kb on + strand, within BWI76_RS02555 at 524.071 kb on + strand, within BWI76_RS02555 at 524.125 kb on - strand, within BWI76_RS02555 at 524.172 kb on + strand, within BWI76_RS02555 at 524.180 kb on - strand, within BWI76_RS02555 at 524.240 kb on + strand at 524.240 kb on + strand, within BWI76_RS02555 at 524.240 kb on + strand, within BWI76_RS02555 at 524.248 kb on - strand, within BWI76_RS02555 at 524.259 kb on - strand, within BWI76_RS02555 at 524.361 kb on - strand at 524.361 kb on - strand at 524.420 kb on + strand at 524.486 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.492 kb on + strand at 524.492 kb on + strand at 524.492 kb on + strand at 524.492 kb on + strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.555 kb on + strand, within BWI76_RS02560 at 524.555 kb on + strand, within BWI76_RS02560 at 524.556 kb on + strand, within BWI76_RS02560 at 524.564 kb on - strand, within BWI76_RS02560 at 524.580 kb on - strand, within BWI76_RS02560 at 524.585 kb on + strand, within BWI76_RS02560 at 524.721 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 522,172 - BWI76_RS02545 0.39 -0.9 522,186 - BWI76_RS02545 0.40 -1.1 522,375 + BWI76_RS02545 0.55 -0.5 522,375 + BWI76_RS02545 0.55 -0.3 522,375 + BWI76_RS02545 0.55 -1.7 522,383 - BWI76_RS02545 0.55 -2.2 522,576 - BWI76_RS02545 0.71 -2.4 522,592 + BWI76_RS02545 0.72 -0.2 522,628 + BWI76_RS02545 0.75 -0.1 522,628 + BWI76_RS02545 0.75 -3.1 522,712 + BWI76_RS02545 0.82 -1.1 522,898 - -0.6 523,054 + BWI76_RS02550 0.11 -0.2 523,078 + BWI76_RS02550 0.13 -1.6 523,078 + BWI76_RS02550 0.13 +0.9 523,359 - BWI76_RS02550 0.41 -1.6 523,388 + BWI76_RS02550 0.44 -2.7 523,677 - BWI76_RS02550 0.73 -1.8 523,828 + BWI76_RS02550 0.88 -2.0 523,874 + -1.0 523,946 + +0.3 524,042 + +1.1 524,048 + BWI76_RS02555 0.12 +0.4 524,067 + BWI76_RS02555 0.18 +0.3 524,071 + BWI76_RS02555 0.20 -0.1 524,125 - BWI76_RS02555 0.38 +0.2 524,172 + BWI76_RS02555 0.54 +0.4 524,180 - BWI76_RS02555 0.57 -0.0 524,240 + -0.0 524,240 + BWI76_RS02555 0.78 -0.2 524,240 + BWI76_RS02555 0.78 +0.5 524,248 - BWI76_RS02555 0.80 -0.2 524,259 - BWI76_RS02555 0.84 +0.6 524,361 - -0.9 524,361 - -0.5 524,420 + -3.0 524,486 + -0.1 524,487 + -1.6 524,487 + -0.2 524,487 + -0.9 524,487 + -0.0 524,487 + +1.0 524,487 + +1.0 524,487 + +0.5 524,487 + -0.7 524,487 + -0.3 524,487 + +0.2 524,487 + -1.0 524,487 + +0.4 524,487 + -0.9 524,487 + +0.7 524,487 + +0.1 524,487 + +1.3 524,487 + +0.3 524,487 + +1.0 524,487 + -0.3 524,487 + +1.2 524,487 + -1.0 524,487 + +0.3 524,487 + -0.4 524,487 + +0.6 524,487 + +0.8 524,487 + +0.7 524,487 + +0.6 524,492 + +0.2 524,492 + +3.0 524,492 + +0.7 524,492 + -0.8 524,495 - +0.5 524,495 - -1.4 524,495 - +1.0 524,495 - +0.6 524,495 - +0.6 524,495 - -0.4 524,495 - -0.6 524,495 - +0.1 524,495 - +1.2 524,495 - +0.2 524,495 - -2.2 524,495 - -0.8 524,495 - -0.3 524,495 - +0.7 524,495 - +0.4 524,495 - +0.7 524,495 - -1.4 524,495 - +0.6 524,495 - +0.6 524,495 - +1.0 524,495 - +0.7 524,495 - +0.0 524,495 - -0.1 524,495 - +0.3 524,495 - +1.1 524,495 - +0.5 524,495 - +0.3 524,495 - -0.1 524,495 - -0.6 524,495 - +0.2 524,495 - -0.0 524,495 - -0.2 524,500 - +0.3 524,500 - -0.5 524,500 - -0.8 524,500 - +0.6 524,500 - -0.0 524,500 - -0.5 524,500 - +0.1 524,500 - -2.6 524,555 + BWI76_RS02560 0.16 +0.4 524,555 + BWI76_RS02560 0.16 +0.7 524,556 + BWI76_RS02560 0.17 -2.5 524,564 - BWI76_RS02560 0.21 +0.1 524,580 - BWI76_RS02560 0.29 -0.1 524,585 + BWI76_RS02560 0.31 +1.0 524,721 + +0.2
Or see this region's nucleotide sequence