Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01200 and BWI76_RS01205 overlap by 4 nucleotides BWI76_RS01205 and BWI76_RS01210 overlap by 4 nucleotides BWI76_RS01210 and BWI76_RS01215 overlap by 1 nucleotides BWI76_RS01215 and BWI76_RS01220 are separated by 77 nucleotides
BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184
_RS01200
BWI76_RS01205: BWI76_RS01205 - DUF484 family protein, at 246,181 to 246,888
_RS01205
BWI76_RS01210: BWI76_RS01210 - tyrosine recombinase XerC, at 246,885 to 247,787
_RS01210
BWI76_RS01215: BWI76_RS01215 - flavin mononucleotide phosphatase, at 247,787 to 248,503
_RS01215
BWI76_RS01220: BWI76_RS01220 - DNA-dependent helicase II, at 248,581 to 250,743
_RS01220
Position (kb) 246
247
248 Strain fitness (log2 ratio) -1
0
1 at 246.061 kb on - strand, within BWI76_RS01200 at 246.133 kb on + strand at 246.133 kb on + strand at 246.133 kb on + strand at 246.233 kb on + strand at 246.238 kb on + strand at 246.744 kb on + strand, within BWI76_RS01205 at 246.900 kb on - strand at 247.091 kb on + strand, within BWI76_RS01210 at 247.448 kb on + strand, within BWI76_RS01210 at 247.460 kb on + strand, within BWI76_RS01210 at 247.835 kb on - strand at 247.857 kb on + strand at 247.916 kb on - strand, within BWI76_RS01215
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 246,061 - BWI76_RS01200 0.85 -0.6 246,133 + +1.0 246,133 + +1.0 246,133 + -0.0 246,233 + -0.0 246,238 + +0.4 246,744 + BWI76_RS01205 0.80 +0.0 246,900 - -1.0 247,091 + BWI76_RS01210 0.23 -1.6 247,448 + BWI76_RS01210 0.62 -1.0 247,460 + BWI76_RS01210 0.64 +1.0 247,835 - +0.6 247,857 + -0.6 247,916 - BWI76_RS01215 0.18 +0.2
Or see this region's nucleotide sequence