Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00715 and BWI76_RS00720 overlap by 4 nucleotides BWI76_RS00720 and BWI76_RS00725 are separated by 149 nucleotides BWI76_RS00725 and BWI76_RS00730 are separated by 149 nucleotides
BWI76_RS00715: BWI76_RS00715 - two-component sensor histidine kinase, at 145,529 to 146,902
_RS00715
BWI76_RS00720: BWI76_RS00720 - DNA-binding response regulator, at 146,899 to 147,597
_RS00720
BWI76_RS00725: BWI76_RS00725 - periplasmic repressor CpxP, at 147,747 to 148,250
_RS00725
BWI76_RS00730: BWI76_RS00730 - cation-efflux pump FieF, at 148,400 to 149,296
_RS00730
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1 at 146.062 kb on + strand, within BWI76_RS00715 at 146.068 kb on + strand, within BWI76_RS00715 at 146.146 kb on - strand at 146.232 kb on - strand, within BWI76_RS00715 at 146.232 kb on - strand, within BWI76_RS00715 at 146.345 kb on - strand, within BWI76_RS00715 at 146.348 kb on + strand, within BWI76_RS00715 at 146.554 kb on + strand, within BWI76_RS00715 at 147.037 kb on + strand, within BWI76_RS00720 at 147.129 kb on + strand, within BWI76_RS00720 at 147.356 kb on - strand, within BWI76_RS00720 at 147.524 kb on - strand, within BWI76_RS00720 at 147.661 kb on + strand at 147.669 kb on - strand at 147.669 kb on - strand at 147.861 kb on - strand, within BWI76_RS00725 at 147.897 kb on - strand, within BWI76_RS00725 at 148.215 kb on + strand at 148.368 kb on + strand at 148.411 kb on - strand at 148.416 kb on - strand at 148.431 kb on - strand at 148.453 kb on + strand at 148.500 kb on - strand, within BWI76_RS00730 at 148.503 kb on - strand, within BWI76_RS00730 at 148.575 kb on - strand, within BWI76_RS00730
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 146,062 + BWI76_RS00715 0.39 -1.0 146,068 + BWI76_RS00715 0.39 +0.0 146,146 - +1.0 146,232 - BWI76_RS00715 0.51 -1.0 146,232 - BWI76_RS00715 0.51 -2.4 146,345 - BWI76_RS00715 0.59 +1.0 146,348 + BWI76_RS00715 0.60 +1.0 146,554 + BWI76_RS00715 0.75 +1.0 147,037 + BWI76_RS00720 0.20 -2.0 147,129 + BWI76_RS00720 0.33 -1.7 147,356 - BWI76_RS00720 0.65 +1.0 147,524 - BWI76_RS00720 0.89 -1.0 147,661 + +1.2 147,669 - -2.0 147,669 - -1.8 147,861 - BWI76_RS00725 0.23 -0.3 147,897 - BWI76_RS00725 0.30 +0.2 148,215 + -2.0 148,368 + +0.3 148,411 - +0.2 148,416 - -1.0 148,431 - +0.1 148,453 + +0.8 148,500 - BWI76_RS00730 0.11 -0.5 148,503 - BWI76_RS00730 0.11 +1.0 148,575 - BWI76_RS00730 0.20 -0.3
Or see this region's nucleotide sequence