Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5674 and Pf6N2E2_5675 are separated by 146 nucleotides Pf6N2E2_5675 and Pf6N2E2_5676 are separated by 91 nucleotides
Pf6N2E2_5674 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 6,486,178 to 6,487,182
_5674
Pf6N2E2_5675 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 6,487,329 to 6,489,950
_5675
Pf6N2E2_5676 - Aspartokinase (EC 2.7.2.4), at 6,490,042 to 6,491,283
_5676
Position (kb)
6487
6488
6489
6490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6486.366 kb on + strand, within Pf6N2E2_5674 at 6486.367 kb on - strand, within Pf6N2E2_5674 at 6486.409 kb on + strand, within Pf6N2E2_5674 at 6486.409 kb on + strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.436 kb on + strand, within Pf6N2E2_5674 at 6486.436 kb on + strand, within Pf6N2E2_5674 at 6486.436 kb on + strand, within Pf6N2E2_5674 at 6486.437 kb on - strand, within Pf6N2E2_5674 at 6486.437 kb on - strand, within Pf6N2E2_5674 at 6486.437 kb on - strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.625 kb on + strand, within Pf6N2E2_5674 at 6486.625 kb on + strand, within Pf6N2E2_5674 at 6486.626 kb on - strand, within Pf6N2E2_5674 at 6486.626 kb on - strand, within Pf6N2E2_5674 at 6486.626 kb on - strand, within Pf6N2E2_5674 at 6486.695 kb on - strand, within Pf6N2E2_5674 at 6486.901 kb on + strand, within Pf6N2E2_5674 at 6486.901 kb on + strand, within Pf6N2E2_5674 at 6486.901 kb on + strand, within Pf6N2E2_5674 at 6486.902 kb on - strand, within Pf6N2E2_5674 at 6486.986 kb on - strand, within Pf6N2E2_5674 at 6487.018 kb on + strand, within Pf6N2E2_5674 at 6487.018 kb on + strand, within Pf6N2E2_5674 at 6487.019 kb on - strand, within Pf6N2E2_5674 at 6487.019 kb on - strand, within Pf6N2E2_5674 at 6487.211 kb on + strand at 6487.249 kb on + strand at 6487.251 kb on + strand at 6487.251 kb on + strand at 6489.934 kb on + strand at 6489.944 kb on + strand at 6489.948 kb on + strand at 6489.948 kb on + strand at 6489.974 kb on + strand at 6490.006 kb on + strand at 6490.006 kb on + strand at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.770 kb on + strand, within Pf6N2E2_5676
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 6,486,366 + Pf6N2E2_5674 0.19 +0.4 6,486,367 - Pf6N2E2_5674 0.19 +0.8 6,486,409 + Pf6N2E2_5674 0.23 +0.4 6,486,409 + Pf6N2E2_5674 0.23 -0.4 6,486,410 - Pf6N2E2_5674 0.23 +0.9 6,486,410 - Pf6N2E2_5674 0.23 -0.9 6,486,410 - Pf6N2E2_5674 0.23 -2.6 6,486,410 - Pf6N2E2_5674 0.23 -1.3 6,486,410 - Pf6N2E2_5674 0.23 +1.2 6,486,410 - Pf6N2E2_5674 0.23 -0.5 6,486,436 + Pf6N2E2_5674 0.26 -0.7 6,486,436 + Pf6N2E2_5674 0.26 -0.0 6,486,436 + Pf6N2E2_5674 0.26 -1.1 6,486,437 - Pf6N2E2_5674 0.26 +2.0 6,486,437 - Pf6N2E2_5674 0.26 -0.5 6,486,437 - Pf6N2E2_5674 0.26 +0.3 6,486,442 + Pf6N2E2_5674 0.26 -2.2 6,486,442 + Pf6N2E2_5674 0.26 -0.8 6,486,442 + Pf6N2E2_5674 0.26 -0.5 6,486,442 + Pf6N2E2_5674 0.26 -0.0 6,486,442 + Pf6N2E2_5674 0.26 +0.0 6,486,443 - Pf6N2E2_5674 0.26 +0.1 6,486,443 - Pf6N2E2_5674 0.26 +0.2 6,486,443 - Pf6N2E2_5674 0.26 +0.5 6,486,443 - Pf6N2E2_5674 0.26 -0.8 6,486,443 - Pf6N2E2_5674 0.26 +2.0 6,486,625 + Pf6N2E2_5674 0.44 +0.2 6,486,625 + Pf6N2E2_5674 0.44 -1.4 6,486,626 - Pf6N2E2_5674 0.45 -0.0 6,486,626 - Pf6N2E2_5674 0.45 -0.8 6,486,626 - Pf6N2E2_5674 0.45 -0.1 6,486,695 - Pf6N2E2_5674 0.51 -0.3 6,486,901 + Pf6N2E2_5674 0.72 -0.8 6,486,901 + Pf6N2E2_5674 0.72 +0.2 6,486,901 + Pf6N2E2_5674 0.72 -2.7 6,486,902 - Pf6N2E2_5674 0.72 +0.2 6,486,986 - Pf6N2E2_5674 0.80 -0.5 6,487,018 + Pf6N2E2_5674 0.84 -0.8 6,487,018 + Pf6N2E2_5674 0.84 -0.6 6,487,019 - Pf6N2E2_5674 0.84 +1.0 6,487,019 - Pf6N2E2_5674 0.84 -0.8 6,487,211 + -0.2 6,487,249 + +0.9 6,487,251 + +0.5 6,487,251 + -0.8 6,489,934 + -2.6 6,489,944 + -0.9 6,489,948 + -0.6 6,489,948 + -0.5 6,489,974 + +1.3 6,490,006 + +0.1 6,490,006 + -0.8 6,490,767 + Pf6N2E2_5676 0.58 -1.2 6,490,767 + Pf6N2E2_5676 0.58 -1.9 6,490,767 + Pf6N2E2_5676 0.58 -0.8 6,490,767 + Pf6N2E2_5676 0.58 -0.4 6,490,767 + Pf6N2E2_5676 0.58 -0.9 6,490,770 + Pf6N2E2_5676 0.59 -0.2
Or see this region's nucleotide sequence