Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5540

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5538 and Pf6N2E2_5539 overlap by 11 nucleotidesPf6N2E2_5539 and Pf6N2E2_5540 are separated by 14 nucleotidesPf6N2E2_5540 and Pf6N2E2_5541 overlap by 1 nucleotides Pf6N2E2_5538 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 6,354,308 to 6,354,775 _5538 Pf6N2E2_5539 - MotA/TolQ/ExbB proton channel family protein, at 6,354,765 to 6,355,460 _5539 Pf6N2E2_5540 - Tol biopolymer transport system, TolR protein, at 6,355,475 to 6,355,936 _5540 Pf6N2E2_5541 - TolA protein, at 6,355,936 to 6,357,012 _5541 Position (kb) 6355 6356Strain fitness (log2 ratio) -1 0 1at 6354.552 kb on + strand, within Pf6N2E2_5538at 6354.552 kb on + strand, within Pf6N2E2_5538at 6354.673 kb on + strand, within Pf6N2E2_5538at 6354.673 kb on + strand, within Pf6N2E2_5538at 6354.705 kb on + strand, within Pf6N2E2_5538at 6354.705 kb on + strand, within Pf6N2E2_5538at 6354.739 kb on + strandat 6355.436 kb on + strandat 6355.436 kb on + strandat 6355.458 kb on + strandat 6355.458 kb on + strandat 6355.458 kb on + strandat 6355.972 kb on + strandat 6355.972 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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6,354,552 + Pf6N2E2_5538 0.52 -0.3
6,354,552 + Pf6N2E2_5538 0.52 -0.2
6,354,673 + Pf6N2E2_5538 0.78 +0.8
6,354,673 + Pf6N2E2_5538 0.78 -1.5
6,354,705 + Pf6N2E2_5538 0.85 +0.3
6,354,705 + Pf6N2E2_5538 0.85 -0.7
6,354,739 + -0.2
6,355,436 + +1.2
6,355,436 + +1.2
6,355,458 + +0.5
6,355,458 + -0.8
6,355,458 + -1.3
6,355,972 + -1.0
6,355,972 + -0.1

Or see this region's nucleotide sequence