Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4747 and Pf6N2E2_4748 overlap by 4 nucleotides Pf6N2E2_4748 and Pf6N2E2_4749 are separated by 239 nucleotides Pf6N2E2_4749 and Pf6N2E2_4750 are separated by 32 nucleotides
Pf6N2E2_4747 - Transmembrane sensor, at 5,483,604 to 5,484,578
_4747
Pf6N2E2_4748 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,484,575 to 5,485,024
_4748
Pf6N2E2_4749 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 5,485,264 to 5,485,935
_4749
Pf6N2E2_4750 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 5,485,968 to 5,487,908
_4750
Position (kb) 5484
5485
5486 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5484.250 kb on + strand, within Pf6N2E2_4747 at 5484.250 kb on + strand, within Pf6N2E2_4747 at 5484.250 kb on + strand, within Pf6N2E2_4747 at 5484.266 kb on - strand, within Pf6N2E2_4747 at 5484.591 kb on + strand at 5484.591 kb on + strand at 5484.591 kb on + strand at 5484.592 kb on - strand at 5484.592 kb on - strand at 5484.603 kb on + strand at 5484.603 kb on + strand at 5484.603 kb on + strand at 5484.603 kb on + strand at 5484.603 kb on + strand at 5484.603 kb on + strand at 5484.603 kb on + strand at 5484.603 kb on + strand at 5484.604 kb on - strand at 5484.630 kb on + strand, within Pf6N2E2_4748 at 5484.631 kb on - strand, within Pf6N2E2_4748 at 5484.631 kb on - strand, within Pf6N2E2_4748 at 5484.639 kb on - strand, within Pf6N2E2_4748 at 5484.704 kb on - strand, within Pf6N2E2_4748 at 5484.705 kb on + strand, within Pf6N2E2_4748 at 5484.705 kb on + strand, within Pf6N2E2_4748 at 5484.705 kb on + strand, within Pf6N2E2_4748 at 5484.705 kb on + strand, within Pf6N2E2_4748 at 5484.706 kb on - strand, within Pf6N2E2_4748 at 5484.706 kb on - strand, within Pf6N2E2_4748 at 5484.706 kb on - strand, within Pf6N2E2_4748 at 5484.840 kb on + strand, within Pf6N2E2_4748 at 5484.840 kb on + strand, within Pf6N2E2_4748 at 5484.840 kb on + strand, within Pf6N2E2_4748 at 5484.840 kb on + strand, within Pf6N2E2_4748 at 5484.841 kb on - strand, within Pf6N2E2_4748 at 5484.841 kb on - strand, within Pf6N2E2_4748 at 5484.871 kb on - strand, within Pf6N2E2_4748 at 5485.025 kb on + strand at 5485.047 kb on + strand at 5485.047 kb on + strand at 5485.047 kb on + strand at 5485.047 kb on + strand at 5485.048 kb on - strand at 5485.048 kb on - strand at 5485.048 kb on - strand at 5485.049 kb on + strand at 5485.189 kb on + strand at 5485.240 kb on + strand at 5485.240 kb on + strand at 5485.240 kb on + strand at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.545 kb on - strand, within Pf6N2E2_4749 at 5485.545 kb on - strand, within Pf6N2E2_4749 at 5485.978 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 5,484,250 + Pf6N2E2_4747 0.66 +0.8 5,484,250 + Pf6N2E2_4747 0.66 +0.3 5,484,250 + Pf6N2E2_4747 0.66 -0.2 5,484,266 - Pf6N2E2_4747 0.68 +1.1 5,484,591 + -0.1 5,484,591 + -1.6 5,484,591 + -0.1 5,484,592 - -0.1 5,484,592 - +0.4 5,484,603 + +0.3 5,484,603 + -0.0 5,484,603 + +0.7 5,484,603 + -1.1 5,484,603 + +1.4 5,484,603 + -0.4 5,484,603 + -0.1 5,484,603 + +0.1 5,484,604 - +0.3 5,484,630 + Pf6N2E2_4748 0.12 -0.1 5,484,631 - Pf6N2E2_4748 0.12 -0.2 5,484,631 - Pf6N2E2_4748 0.12 -0.1 5,484,639 - Pf6N2E2_4748 0.14 -1.4 5,484,704 - Pf6N2E2_4748 0.29 -0.6 5,484,705 + Pf6N2E2_4748 0.29 -0.4 5,484,705 + Pf6N2E2_4748 0.29 -0.9 5,484,705 + Pf6N2E2_4748 0.29 -0.6 5,484,705 + Pf6N2E2_4748 0.29 +1.0 5,484,706 - Pf6N2E2_4748 0.29 -1.1 5,484,706 - Pf6N2E2_4748 0.29 -1.0 5,484,706 - Pf6N2E2_4748 0.29 +0.7 5,484,840 + Pf6N2E2_4748 0.59 -1.0 5,484,840 + Pf6N2E2_4748 0.59 -0.7 5,484,840 + Pf6N2E2_4748 0.59 -0.0 5,484,840 + Pf6N2E2_4748 0.59 -3.1 5,484,841 - Pf6N2E2_4748 0.59 +0.3 5,484,841 - Pf6N2E2_4748 0.59 -0.4 5,484,871 - Pf6N2E2_4748 0.66 -0.4 5,485,025 + -0.6 5,485,047 + -0.8 5,485,047 + +0.2 5,485,047 + +0.1 5,485,047 + -1.1 5,485,048 - -0.9 5,485,048 - +0.1 5,485,048 - -1.7 5,485,049 + -0.2 5,485,189 + -0.1 5,485,240 + +0.1 5,485,240 + +0.4 5,485,240 + -0.4 5,485,544 + Pf6N2E2_4749 0.42 +0.9 5,485,544 + Pf6N2E2_4749 0.42 +0.8 5,485,544 + Pf6N2E2_4749 0.42 -2.1 5,485,544 + Pf6N2E2_4749 0.42 -0.5 5,485,545 - Pf6N2E2_4749 0.42 +0.3 5,485,545 - Pf6N2E2_4749 0.42 +0.6 5,485,978 + +0.4
Or see this region's nucleotide sequence