Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3456 and Pf6N2E2_3457 overlap by 4 nucleotides Pf6N2E2_3457 and Pf6N2E2_3458 are separated by 139 nucleotides
Pf6N2E2_3456 - Inner membrane protein YfiN, at 4,025,328 to 4,026,596
_3456
Pf6N2E2_3457 - Uncharacterized protein YfiR precursor, at 4,026,593 to 4,027,168
_3457
Pf6N2E2_3458 - Exodeoxyribonuclease V alpha chain (EC 3.1.11.5), at 4,027,308 to 4,027,979
_3458
Position (kb)
4026
4027
4028 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4025.749 kb on + strand, within Pf6N2E2_3456 at 4025.749 kb on + strand, within Pf6N2E2_3456 at 4025.750 kb on - strand, within Pf6N2E2_3456 at 4025.750 kb on - strand, within Pf6N2E2_3456 at 4026.002 kb on + strand, within Pf6N2E2_3456 at 4026.003 kb on - strand, within Pf6N2E2_3456 at 4026.003 kb on - strand, within Pf6N2E2_3456 at 4026.003 kb on - strand, within Pf6N2E2_3456 at 4026.003 kb on - strand, within Pf6N2E2_3456 at 4026.076 kb on + strand, within Pf6N2E2_3456 at 4026.076 kb on + strand, within Pf6N2E2_3456 at 4026.171 kb on + strand, within Pf6N2E2_3456 at 4026.172 kb on - strand, within Pf6N2E2_3456 at 4026.186 kb on - strand, within Pf6N2E2_3456 at 4026.314 kb on + strand, within Pf6N2E2_3456 at 4026.314 kb on + strand, within Pf6N2E2_3456 at 4026.315 kb on - strand, within Pf6N2E2_3456 at 4026.315 kb on - strand, within Pf6N2E2_3456 at 4026.418 kb on + strand, within Pf6N2E2_3456 at 4026.418 kb on + strand, within Pf6N2E2_3456 at 4026.419 kb on - strand, within Pf6N2E2_3456 at 4026.460 kb on + strand, within Pf6N2E2_3456 at 4026.460 kb on + strand, within Pf6N2E2_3456 at 4026.461 kb on - strand, within Pf6N2E2_3456 at 4026.599 kb on + strand at 4026.828 kb on + strand, within Pf6N2E2_3457 at 4026.828 kb on + strand, within Pf6N2E2_3457 at 4026.828 kb on + strand, within Pf6N2E2_3457 at 4026.881 kb on + strand, within Pf6N2E2_3457 at 4026.936 kb on + strand, within Pf6N2E2_3457 at 4026.996 kb on + strand, within Pf6N2E2_3457 at 4026.996 kb on + strand, within Pf6N2E2_3457 at 4026.996 kb on + strand, within Pf6N2E2_3457 at 4027.079 kb on + strand, within Pf6N2E2_3457 at 4027.080 kb on - strand, within Pf6N2E2_3457 at 4027.080 kb on - strand, within Pf6N2E2_3457 at 4027.119 kb on + strand at 4027.119 kb on + strand at 4027.120 kb on - strand at 4027.120 kb on - strand at 4027.120 kb on - strand at 4027.186 kb on + strand at 4027.192 kb on - strand at 4027.192 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 4,025,749 + Pf6N2E2_3456 0.33 +0.8 4,025,749 + Pf6N2E2_3456 0.33 -0.2 4,025,750 - Pf6N2E2_3456 0.33 +1.8 4,025,750 - Pf6N2E2_3456 0.33 +0.7 4,026,002 + Pf6N2E2_3456 0.53 -1.5 4,026,003 - Pf6N2E2_3456 0.53 +1.4 4,026,003 - Pf6N2E2_3456 0.53 -0.1 4,026,003 - Pf6N2E2_3456 0.53 +0.6 4,026,003 - Pf6N2E2_3456 0.53 +0.2 4,026,076 + Pf6N2E2_3456 0.59 -0.3 4,026,076 + Pf6N2E2_3456 0.59 -0.4 4,026,171 + Pf6N2E2_3456 0.66 +0.4 4,026,172 - Pf6N2E2_3456 0.67 +0.0 4,026,186 - Pf6N2E2_3456 0.68 +0.0 4,026,314 + Pf6N2E2_3456 0.78 +0.9 4,026,314 + Pf6N2E2_3456 0.78 +1.4 4,026,315 - Pf6N2E2_3456 0.78 +1.2 4,026,315 - Pf6N2E2_3456 0.78 +1.4 4,026,418 + Pf6N2E2_3456 0.86 -0.5 4,026,418 + Pf6N2E2_3456 0.86 +1.8 4,026,419 - Pf6N2E2_3456 0.86 +1.4 4,026,460 + Pf6N2E2_3456 0.89 +1.7 4,026,460 + Pf6N2E2_3456 0.89 +0.7 4,026,461 - Pf6N2E2_3456 0.89 +0.0 4,026,599 + +1.4 4,026,828 + Pf6N2E2_3457 0.41 +0.2 4,026,828 + Pf6N2E2_3457 0.41 +0.5 4,026,828 + Pf6N2E2_3457 0.41 +0.3 4,026,881 + Pf6N2E2_3457 0.50 +0.5 4,026,936 + Pf6N2E2_3457 0.60 +1.8 4,026,996 + Pf6N2E2_3457 0.70 +0.9 4,026,996 + Pf6N2E2_3457 0.70 +1.2 4,026,996 + Pf6N2E2_3457 0.70 +1.7 4,027,079 + Pf6N2E2_3457 0.84 +1.7 4,027,080 - Pf6N2E2_3457 0.85 +0.6 4,027,080 - Pf6N2E2_3457 0.85 -0.5 4,027,119 + +0.7 4,027,119 + +2.2 4,027,120 - +0.0 4,027,120 - +0.2 4,027,120 - -2.2 4,027,186 + -0.2 4,027,192 - -1.2 4,027,192 - -0.8 4,027,250 - +0.1 4,027,250 - -1.3 4,027,250 - +1.4 4,027,250 - +1.4 4,027,250 - +0.3
Or see this region's nucleotide sequence