Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3085 and Pf6N2E2_3086 are separated by 147 nucleotides Pf6N2E2_3086 and Pf6N2E2_3087 are separated by 234 nucleotides Pf6N2E2_3087 and Pf6N2E2_3088 are separated by 187 nucleotides Pf6N2E2_3088 and Pf6N2E2_3089 are separated by 73 nucleotides
Pf6N2E2_3085 - FIG00954614: hypothetical protein, at 3,572,222 to 3,572,599
_3085
Pf6N2E2_3086 - Cold shock protein CspA, at 3,572,747 to 3,572,956
_3086
Pf6N2E2_3087 - Glycerol-3-phosphate acyltransferase (EC 2.3.1.15), at 3,573,191 to 3,575,689
_3087
Pf6N2E2_3088 - hypothetical protein, at 3,575,877 to 3,576,251
_3088
Pf6N2E2_3089 - Lipoprotein-related protein, at 3,576,325 to 3,576,747
_3089
Position (kb)
3573
3574
3575
3576 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3572.247 kb on - strand at 3572.343 kb on - strand, within Pf6N2E2_3085 at 3572.343 kb on - strand, within Pf6N2E2_3085 at 3572.582 kb on - strand at 3572.582 kb on - strand at 3572.659 kb on - strand at 3572.659 kb on - strand at 3572.673 kb on - strand at 3572.731 kb on - strand at 3572.748 kb on + strand at 3572.749 kb on - strand at 3572.976 kb on - strand at 3572.976 kb on - strand at 3572.980 kb on + strand at 3572.980 kb on + strand at 3572.981 kb on - strand at 3572.981 kb on - strand at 3572.982 kb on + strand at 3572.982 kb on + strand at 3572.982 kb on + strand at 3572.982 kb on + strand at 3572.983 kb on - strand at 3572.983 kb on - strand at 3572.983 kb on - strand at 3572.983 kb on - strand at 3573.009 kb on - strand at 3573.066 kb on + strand at 3573.066 kb on + strand at 3573.066 kb on + strand at 3573.066 kb on + strand at 3573.067 kb on - strand at 3573.067 kb on - strand at 3573.067 kb on - strand at 3573.067 kb on - strand at 3573.068 kb on + strand at 3573.068 kb on + strand at 3573.068 kb on + strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.100 kb on - strand at 3573.142 kb on + strand at 3573.169 kb on + strand at 3573.190 kb on + strand at 3573.191 kb on - strand at 3573.245 kb on + strand at 3573.245 kb on + strand at 3573.246 kb on - strand at 3573.246 kb on - strand at 3573.246 kb on - strand at 3573.246 kb on - strand at 3573.320 kb on + strand at 3573.320 kb on + strand at 3573.320 kb on + strand at 3573.320 kb on + strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.986 kb on + strand, within Pf6N2E2_3087 at 3573.986 kb on + strand, within Pf6N2E2_3087 at 3573.986 kb on + strand, within Pf6N2E2_3087 at 3573.986 kb on + strand, within Pf6N2E2_3087 at 3573.986 kb on + strand, within Pf6N2E2_3087 at 3573.987 kb on - strand, within Pf6N2E2_3087 at 3573.987 kb on - strand, within Pf6N2E2_3087 at 3573.987 kb on - strand, within Pf6N2E2_3087 at 3573.987 kb on - strand, within Pf6N2E2_3087 at 3574.106 kb on + strand, within Pf6N2E2_3087 at 3574.106 kb on + strand, within Pf6N2E2_3087 at 3574.106 kb on + strand, within Pf6N2E2_3087 at 3574.107 kb on - strand, within Pf6N2E2_3087 at 3574.107 kb on - strand, within Pf6N2E2_3087 at 3574.107 kb on - strand, within Pf6N2E2_3087 at 3574.136 kb on + strand, within Pf6N2E2_3087 at 3574.151 kb on + strand, within Pf6N2E2_3087 at 3574.151 kb on + strand, within Pf6N2E2_3087 at 3574.152 kb on - strand, within Pf6N2E2_3087 at 3574.152 kb on - strand, within Pf6N2E2_3087 at 3574.287 kb on - strand, within Pf6N2E2_3087 at 3574.307 kb on + strand, within Pf6N2E2_3087 at 3574.308 kb on - strand, within Pf6N2E2_3087 at 3574.308 kb on - strand, within Pf6N2E2_3087 at 3574.346 kb on + strand, within Pf6N2E2_3087 at 3574.346 kb on + strand, within Pf6N2E2_3087 at 3574.346 kb on + strand, within Pf6N2E2_3087 at 3574.346 kb on + strand, within Pf6N2E2_3087 at 3574.347 kb on - strand, within Pf6N2E2_3087 at 3574.469 kb on + strand, within Pf6N2E2_3087 at 3574.469 kb on + strand, within Pf6N2E2_3087 at 3574.470 kb on - strand, within Pf6N2E2_3087 at 3574.470 kb on - strand, within Pf6N2E2_3087 at 3574.470 kb on - strand, within Pf6N2E2_3087 at 3574.470 kb on - strand, within Pf6N2E2_3087 at 3574.505 kb on + strand, within Pf6N2E2_3087 at 3574.505 kb on + strand, within Pf6N2E2_3087 at 3574.506 kb on - strand, within Pf6N2E2_3087 at 3574.857 kb on - strand, within Pf6N2E2_3087 at 3574.857 kb on - strand, within Pf6N2E2_3087 at 3574.938 kb on + strand, within Pf6N2E2_3087 at 3574.938 kb on + strand, within Pf6N2E2_3087 at 3574.938 kb on + strand, within Pf6N2E2_3087 at 3574.939 kb on - strand, within Pf6N2E2_3087 at 3574.939 kb on - strand, within Pf6N2E2_3087 at 3574.939 kb on - strand, within Pf6N2E2_3087 at 3574.939 kb on - strand, within Pf6N2E2_3087 at 3574.939 kb on - strand, within Pf6N2E2_3087 at 3574.939 kb on - strand, within Pf6N2E2_3087 at 3574.992 kb on - strand, within Pf6N2E2_3087 at 3575.013 kb on + strand, within Pf6N2E2_3087 at 3575.024 kb on + strand, within Pf6N2E2_3087 at 3575.024 kb on + strand, within Pf6N2E2_3087 at 3575.024 kb on + strand, within Pf6N2E2_3087 at 3575.025 kb on - strand, within Pf6N2E2_3087 at 3575.027 kb on + strand, within Pf6N2E2_3087 at 3575.028 kb on - strand, within Pf6N2E2_3087 at 3575.138 kb on + strand, within Pf6N2E2_3087 at 3575.145 kb on - strand, within Pf6N2E2_3087 at 3575.258 kb on + strand, within Pf6N2E2_3087 at 3575.258 kb on + strand, within Pf6N2E2_3087 at 3575.258 kb on + strand, within Pf6N2E2_3087 at 3575.259 kb on - strand, within Pf6N2E2_3087 at 3575.342 kb on + strand, within Pf6N2E2_3087 at 3575.342 kb on + strand, within Pf6N2E2_3087 at 3575.342 kb on + strand, within Pf6N2E2_3087 at 3575.343 kb on - strand, within Pf6N2E2_3087 at 3575.343 kb on - strand, within Pf6N2E2_3087 at 3575.520 kb on + strand at 3575.520 kb on + strand at 3575.520 kb on + strand at 3575.521 kb on - strand at 3575.552 kb on + strand at 3575.552 kb on + strand at 3575.552 kb on + strand at 3575.553 kb on - strand at 3575.553 kb on - strand at 3575.553 kb on - strand at 3575.553 kb on - strand at 3575.757 kb on - strand at 3575.807 kb on - strand at 3575.807 kb on - strand at 3575.831 kb on + strand at 3575.832 kb on - strand at 3575.832 kb on - strand at 3575.832 kb on - strand at 3575.832 kb on - strand at 3575.858 kb on + strand at 3575.858 kb on + strand at 3575.858 kb on + strand at 3575.859 kb on - strand at 3576.014 kb on + strand, within Pf6N2E2_3088 at 3576.015 kb on - strand, within Pf6N2E2_3088 at 3576.262 kb on + strand at 3576.291 kb on + strand at 3576.292 kb on - strand at 3576.378 kb on - strand, within Pf6N2E2_3089 at 3576.378 kb on - strand, within Pf6N2E2_3089 at 3576.378 kb on - strand, within Pf6N2E2_3089 at 3576.445 kb on + strand, within Pf6N2E2_3089 at 3576.445 kb on + strand, within Pf6N2E2_3089 at 3576.445 kb on + strand, within Pf6N2E2_3089 at 3576.446 kb on - strand, within Pf6N2E2_3089 at 3576.446 kb on - strand, within Pf6N2E2_3089 at 3576.478 kb on - strand, within Pf6N2E2_3089 at 3576.589 kb on + strand, within Pf6N2E2_3089 at 3576.589 kb on + strand, within Pf6N2E2_3089 at 3576.589 kb on + strand, within Pf6N2E2_3089 at 3576.589 kb on + strand, within Pf6N2E2_3089 at 3576.590 kb on - strand, within Pf6N2E2_3089 at 3576.590 kb on - strand, within Pf6N2E2_3089 at 3576.590 kb on - strand, within Pf6N2E2_3089 at 3576.590 kb on - strand, within Pf6N2E2_3089 at 3576.623 kb on - strand, within Pf6N2E2_3089
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 3,572,247 - -0.1 3,572,343 - Pf6N2E2_3085 0.32 +0.6 3,572,343 - Pf6N2E2_3085 0.32 -0.5 3,572,582 - +0.2 3,572,582 - +0.8 3,572,659 - +0.4 3,572,659 - +1.2 3,572,673 - -0.8 3,572,731 - -2.4 3,572,748 + -0.1 3,572,749 - -0.3 3,572,976 - -1.4 3,572,976 - +0.1 3,572,980 + -0.5 3,572,980 + +0.8 3,572,981 - -0.0 3,572,981 - -0.1 3,572,982 + -0.4 3,572,982 + -1.4 3,572,982 + +1.2 3,572,982 + +1.2 3,572,983 - -0.5 3,572,983 - -0.2 3,572,983 - -1.8 3,572,983 - +2.2 3,573,009 - +0.5 3,573,066 + -0.1 3,573,066 + -0.8 3,573,066 + -0.4 3,573,066 + +1.8 3,573,067 - -0.9 3,573,067 - -1.4 3,573,067 - -0.1 3,573,067 - -0.8 3,573,068 + +0.6 3,573,068 + +2.8 3,573,068 + -2.1 3,573,069 - -1.6 3,573,069 - -1.1 3,573,069 - -0.0 3,573,069 - +0.6 3,573,069 - -0.5 3,573,100 - -0.4 3,573,142 + +0.2 3,573,169 + -0.9 3,573,190 + +1.5 3,573,191 - -0.5 3,573,245 + -1.8 3,573,245 + -1.0 3,573,246 - -0.8 3,573,246 - +0.2 3,573,246 - -0.9 3,573,246 - -0.2 3,573,320 + -0.4 3,573,320 + -0.6 3,573,320 + +0.3 3,573,320 + -0.4 3,573,321 - -0.1 3,573,321 - -0.2 3,573,321 - -0.8 3,573,321 - -0.6 3,573,321 - -0.5 3,573,321 - -0.3 3,573,321 - -0.5 3,573,986 + Pf6N2E2_3087 0.32 -0.9 3,573,986 + Pf6N2E2_3087 0.32 +0.5 3,573,986 + Pf6N2E2_3087 0.32 -0.3 3,573,986 + Pf6N2E2_3087 0.32 +0.5 3,573,986 + Pf6N2E2_3087 0.32 -1.4 3,573,987 - Pf6N2E2_3087 0.32 +0.1 3,573,987 - Pf6N2E2_3087 0.32 -0.6 3,573,987 - Pf6N2E2_3087 0.32 +0.4 3,573,987 - Pf6N2E2_3087 0.32 -0.9 3,574,106 + Pf6N2E2_3087 0.37 -0.2 3,574,106 + Pf6N2E2_3087 0.37 -1.0 3,574,106 + Pf6N2E2_3087 0.37 +0.2 3,574,107 - Pf6N2E2_3087 0.37 +0.3 3,574,107 - Pf6N2E2_3087 0.37 -0.0 3,574,107 - Pf6N2E2_3087 0.37 +0.8 3,574,136 + Pf6N2E2_3087 0.38 -0.6 3,574,151 + Pf6N2E2_3087 0.38 -0.3 3,574,151 + Pf6N2E2_3087 0.38 +1.1 3,574,152 - Pf6N2E2_3087 0.38 -1.7 3,574,152 - Pf6N2E2_3087 0.38 +1.2 3,574,287 - Pf6N2E2_3087 0.44 -0.8 3,574,307 + Pf6N2E2_3087 0.45 -0.2 3,574,308 - Pf6N2E2_3087 0.45 -0.6 3,574,308 - Pf6N2E2_3087 0.45 -0.0 3,574,346 + Pf6N2E2_3087 0.46 -1.4 3,574,346 + Pf6N2E2_3087 0.46 -0.6 3,574,346 + Pf6N2E2_3087 0.46 -0.8 3,574,346 + Pf6N2E2_3087 0.46 -0.1 3,574,347 - Pf6N2E2_3087 0.46 -0.2 3,574,469 + Pf6N2E2_3087 0.51 +0.2 3,574,469 + Pf6N2E2_3087 0.51 -1.3 3,574,470 - Pf6N2E2_3087 0.51 -0.9 3,574,470 - Pf6N2E2_3087 0.51 -0.0 3,574,470 - Pf6N2E2_3087 0.51 +0.2 3,574,470 - Pf6N2E2_3087 0.51 -0.8 3,574,505 + Pf6N2E2_3087 0.53 -0.4 3,574,505 + Pf6N2E2_3087 0.53 -0.5 3,574,506 - Pf6N2E2_3087 0.53 -1.5 3,574,857 - Pf6N2E2_3087 0.67 +0.2 3,574,857 - Pf6N2E2_3087 0.67 +0.2 3,574,938 + Pf6N2E2_3087 0.70 +0.8 3,574,938 + Pf6N2E2_3087 0.70 -0.5 3,574,938 + Pf6N2E2_3087 0.70 -0.9 3,574,939 - Pf6N2E2_3087 0.70 +0.2 3,574,939 - Pf6N2E2_3087 0.70 +0.2 3,574,939 - Pf6N2E2_3087 0.70 +0.7 3,574,939 - Pf6N2E2_3087 0.70 +0.2 3,574,939 - Pf6N2E2_3087 0.70 +0.4 3,574,939 - Pf6N2E2_3087 0.70 -0.3 3,574,992 - Pf6N2E2_3087 0.72 +1.2 3,575,013 + Pf6N2E2_3087 0.73 -1.6 3,575,024 + Pf6N2E2_3087 0.73 -0.1 3,575,024 + Pf6N2E2_3087 0.73 -1.3 3,575,024 + Pf6N2E2_3087 0.73 +0.9 3,575,025 - Pf6N2E2_3087 0.73 -0.1 3,575,027 + Pf6N2E2_3087 0.73 -0.3 3,575,028 - Pf6N2E2_3087 0.74 -0.8 3,575,138 + Pf6N2E2_3087 0.78 -0.0 3,575,145 - Pf6N2E2_3087 0.78 -1.5 3,575,258 + Pf6N2E2_3087 0.83 +0.0 3,575,258 + Pf6N2E2_3087 0.83 -0.2 3,575,258 + Pf6N2E2_3087 0.83 -0.6 3,575,259 - Pf6N2E2_3087 0.83 -0.2 3,575,342 + Pf6N2E2_3087 0.86 -0.1 3,575,342 + Pf6N2E2_3087 0.86 -0.5 3,575,342 + Pf6N2E2_3087 0.86 +1.7 3,575,343 - Pf6N2E2_3087 0.86 +0.5 3,575,343 - Pf6N2E2_3087 0.86 -0.5 3,575,520 + -0.4 3,575,520 + +2.2 3,575,520 + -0.8 3,575,521 - +0.0 3,575,552 + +0.2 3,575,552 + -0.0 3,575,552 + -0.4 3,575,553 - -0.3 3,575,553 - -0.4 3,575,553 - -0.5 3,575,553 - -1.0 3,575,757 - -3.1 3,575,807 - -1.1 3,575,807 - -0.1 3,575,831 + -0.5 3,575,832 - +1.4 3,575,832 - +0.4 3,575,832 - +0.6 3,575,832 - -0.7 3,575,858 + -0.4 3,575,858 + +0.9 3,575,858 + -0.5 3,575,859 - -1.9 3,576,014 + Pf6N2E2_3088 0.37 +0.5 3,576,015 - Pf6N2E2_3088 0.37 +0.4 3,576,262 + +0.4 3,576,291 + -0.4 3,576,292 - +0.6 3,576,378 - Pf6N2E2_3089 0.13 -1.3 3,576,378 - Pf6N2E2_3089 0.13 -1.6 3,576,378 - Pf6N2E2_3089 0.13 -0.8 3,576,445 + Pf6N2E2_3089 0.28 +0.4 3,576,445 + Pf6N2E2_3089 0.28 +1.2 3,576,445 + Pf6N2E2_3089 0.28 +1.9 3,576,446 - Pf6N2E2_3089 0.29 -0.1 3,576,446 - Pf6N2E2_3089 0.29 +0.1 3,576,478 - Pf6N2E2_3089 0.36 -1.2 3,576,589 + Pf6N2E2_3089 0.62 -1.3 3,576,589 + Pf6N2E2_3089 0.62 +1.2 3,576,589 + Pf6N2E2_3089 0.62 +2.2 3,576,589 + Pf6N2E2_3089 0.62 +0.2 3,576,590 - Pf6N2E2_3089 0.63 -0.6 3,576,590 - Pf6N2E2_3089 0.63 -0.2 3,576,590 - Pf6N2E2_3089 0.63 +0.6 3,576,590 - Pf6N2E2_3089 0.63 -0.8 3,576,623 - Pf6N2E2_3089 0.70 -0.1
Or see this region's nucleotide sequence