Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_173

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_171 and Pf6N2E2_172 are separated by 289 nucleotidesPf6N2E2_172 and Pf6N2E2_173 are separated by 371 nucleotidesPf6N2E2_173 and Pf6N2E2_174 are separated by 17 nucleotidesPf6N2E2_174 and Pf6N2E2_175 are separated by 60 nucleotidesPf6N2E2_175 and Pf6N2E2_176 are separated by 30 nucleotides Pf6N2E2_171 - Cold shock protein CspG, at 222,470 to 222,682 _171 Pf6N2E2_172 - FIG00953292: hypothetical protein, at 222,972 to 223,274 _172 Pf6N2E2_173 - Threonyl-tRNA synthetase (EC 6.1.1.3), at 223,646 to 225,568 _173 Pf6N2E2_174 - Translation initiation factor 3, at 225,586 to 226,119 _174 Pf6N2E2_175 - LSU ribosomal protein L35p, at 226,180 to 226,374 _175 Pf6N2E2_176 - LSU ribosomal protein L20p, at 226,405 to 226,761 _176 Position (kb) 223 224 225 226Strain fitness (log2 ratio) -2 -1 0 1 2at 222.687 kb on + strandat 222.703 kb on - strandat 222.703 kb on - strandat 222.704 kb on + strandat 222.704 kb on + strandat 222.705 kb on - strandat 222.715 kb on + strandat 222.715 kb on + strandat 222.716 kb on - strandat 222.716 kb on - strandat 222.755 kb on + strandat 222.903 kb on - strandat 222.903 kb on - strandat 222.903 kb on - strandat 223.084 kb on + strand, within Pf6N2E2_172at 223.085 kb on - strand, within Pf6N2E2_172at 223.085 kb on - strand, within Pf6N2E2_172at 223.177 kb on + strand, within Pf6N2E2_172at 223.178 kb on - strand, within Pf6N2E2_172at 223.178 kb on - strand, within Pf6N2E2_172at 223.178 kb on - strand, within Pf6N2E2_172at 223.178 kb on - strand, within Pf6N2E2_172at 223.249 kb on - strandat 223.262 kb on + strandat 223.262 kb on + strandat 223.262 kb on + strandat 223.262 kb on + strandat 223.263 kb on - strandat 223.263 kb on - strandat 223.263 kb on - strandat 223.263 kb on - strandat 223.263 kb on - strandat 223.349 kb on - strandat 223.349 kb on - strandat 223.363 kb on + strandat 223.363 kb on + strandat 223.363 kb on + strandat 223.363 kb on + strandat 223.364 kb on - strandat 223.364 kb on - strandat 223.408 kb on + strandat 223.408 kb on + strandat 226.117 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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222,687 + -0.1
222,703 - +0.7
222,703 - +0.3
222,704 + -0.4
222,704 + -1.9
222,705 - -0.1
222,715 + -1.4
222,715 + -0.6
222,716 - -1.1
222,716 - -1.4
222,755 + +1.9
222,903 - -0.7
222,903 - -1.0
222,903 - +0.4
223,084 + Pf6N2E2_172 0.37 -0.1
223,085 - Pf6N2E2_172 0.37 +0.2
223,085 - Pf6N2E2_172 0.37 -0.1
223,177 + Pf6N2E2_172 0.68 -0.1
223,178 - Pf6N2E2_172 0.68 +0.4
223,178 - Pf6N2E2_172 0.68 -2.2
223,178 - Pf6N2E2_172 0.68 +1.2
223,178 - Pf6N2E2_172 0.68 +0.4
223,249 - +0.4
223,262 + +0.4
223,262 + -1.3
223,262 + -1.6
223,262 + +0.9
223,263 - +0.4
223,263 - -0.3
223,263 - -1.2
223,263 - -0.3
223,263 - -1.6
223,349 - -1.5
223,349 - +0.4
223,363 + -0.8
223,363 + -0.5
223,363 + -1.9
223,363 + -0.1
223,364 - +0.1
223,364 - +0.9
223,408 + -0.6
223,408 + +0.7
226,117 + -0.2

Or see this region's nucleotide sequence