Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27100 and BWI76_RS27105 overlap by 4 nucleotides BWI76_RS27105 and BWI76_RS27110 overlap by 8 nucleotides BWI76_RS27110 and BWI76_RS27115 overlap by 7 nucleotides
BWI76_RS27100: BWI76_RS27100 - carbohydrate kinase, at 5,623,536 to 5,625,041
_RS27100
BWI76_RS27105: BWI76_RS27105 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 5,625,038 to 5,625,700
_RS27105
BWI76_RS27110: BWI76_RS27110 - xylulose 5-phosphate 3-epimerase, at 5,625,693 to 5,626,553
_RS27110
BWI76_RS27115: BWI76_RS27115 - L-ribulose-5-phosphate 4-epimerase, at 5,626,547 to 5,627,245
_RS27115
Position (kb) 5625
5626 Strain fitness (log2 ratio) -1
0
1
2 at 5624.175 kb on + strand, within BWI76_RS27100 at 5624.183 kb on - strand, within BWI76_RS27100 at 5624.241 kb on - strand, within BWI76_RS27100 at 5624.281 kb on + strand, within BWI76_RS27100 at 5624.377 kb on + strand, within BWI76_RS27100 at 5624.390 kb on + strand, within BWI76_RS27100 at 5624.398 kb on - strand, within BWI76_RS27100 at 5624.405 kb on - strand, within BWI76_RS27100 at 5624.474 kb on + strand, within BWI76_RS27100 at 5624.603 kb on - strand, within BWI76_RS27100 at 5624.609 kb on - strand, within BWI76_RS27100 at 5624.872 kb on + strand, within BWI76_RS27100 at 5624.879 kb on - strand, within BWI76_RS27100 at 5624.944 kb on + strand at 5625.000 kb on + strand at 5625.000 kb on + strand at 5625.013 kb on - strand at 5625.019 kb on + strand at 5625.079 kb on + strand at 5625.099 kb on + strand at 5625.349 kb on - strand, within BWI76_RS27105 at 5625.416 kb on - strand, within BWI76_RS27105 at 5625.458 kb on + strand, within BWI76_RS27105 at 5625.475 kb on + strand, within BWI76_RS27105 at 5625.525 kb on + strand, within BWI76_RS27105 at 5625.549 kb on + strand, within BWI76_RS27105 at 5625.557 kb on - strand, within BWI76_RS27105 at 5625.582 kb on - strand, within BWI76_RS27105 at 5625.590 kb on + strand, within BWI76_RS27105 at 5625.619 kb on + strand, within BWI76_RS27105 at 5625.743 kb on + strand at 5625.756 kb on - strand at 5625.852 kb on - strand, within BWI76_RS27110 at 5625.869 kb on - strand, within BWI76_RS27110 at 5625.947 kb on + strand, within BWI76_RS27110 at 5625.991 kb on - strand, within BWI76_RS27110 at 5626.103 kb on - strand, within BWI76_RS27110 at 5626.118 kb on + strand at 5626.258 kb on - strand at 5626.334 kb on + strand, within BWI76_RS27110 at 5626.351 kb on + strand, within BWI76_RS27110 at 5626.351 kb on + strand, within BWI76_RS27110 at 5626.508 kb on + strand at 5626.614 kb on + strand at 5626.614 kb on + strand at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.693 kb on + strand, within BWI76_RS27115
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,624,175 + BWI76_RS27100 0.42 +0.6 5,624,183 - BWI76_RS27100 0.43 -1.0 5,624,241 - BWI76_RS27100 0.47 -0.0 5,624,281 + BWI76_RS27100 0.49 +0.0 5,624,377 + BWI76_RS27100 0.56 +0.9 5,624,390 + BWI76_RS27100 0.57 -0.1 5,624,398 - BWI76_RS27100 0.57 +0.5 5,624,405 - BWI76_RS27100 0.58 -0.4 5,624,474 + BWI76_RS27100 0.62 +0.4 5,624,603 - BWI76_RS27100 0.71 -0.1 5,624,609 - BWI76_RS27100 0.71 -0.9 5,624,872 + BWI76_RS27100 0.89 -0.0 5,624,879 - BWI76_RS27100 0.89 +1.0 5,624,944 + -0.6 5,625,000 + +0.6 5,625,000 + +0.7 5,625,013 - -0.6 5,625,019 + -0.2 5,625,079 + -0.0 5,625,099 + -1.2 5,625,349 - BWI76_RS27105 0.47 +0.2 5,625,416 - BWI76_RS27105 0.57 +0.4 5,625,458 + BWI76_RS27105 0.63 +0.4 5,625,475 + BWI76_RS27105 0.66 +0.4 5,625,525 + BWI76_RS27105 0.73 -1.0 5,625,549 + BWI76_RS27105 0.77 +0.4 5,625,557 - BWI76_RS27105 0.78 +0.3 5,625,582 - BWI76_RS27105 0.82 +1.1 5,625,590 + BWI76_RS27105 0.83 +0.4 5,625,619 + BWI76_RS27105 0.88 +0.2 5,625,743 + -1.5 5,625,756 - +0.3 5,625,852 - BWI76_RS27110 0.18 -0.2 5,625,869 - BWI76_RS27110 0.20 -0.8 5,625,947 + BWI76_RS27110 0.30 +0.3 5,625,991 - BWI76_RS27110 0.35 -0.9 5,626,103 - BWI76_RS27110 0.48 +0.4 5,626,118 + -1.3 5,626,258 - -0.7 5,626,334 + BWI76_RS27110 0.74 -1.7 5,626,351 + BWI76_RS27110 0.76 -1.2 5,626,351 + BWI76_RS27110 0.76 -0.8 5,626,508 + +0.4 5,626,614 + +1.0 5,626,614 + +2.7 5,626,686 + BWI76_RS27115 0.20 +0.9 5,626,686 + BWI76_RS27115 0.20 -0.4 5,626,686 + BWI76_RS27115 0.20 +0.0 5,626,693 + BWI76_RS27115 0.21 -0.3
Or see this region's nucleotide sequence