Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23255 and BWI76_RS23260 overlap by 10 nucleotides BWI76_RS23260 and BWI76_RS23265 are separated by 218 nucleotides BWI76_RS23265 and BWI76_RS23270 are separated by 227 nucleotides
BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503
_RS23255
BWI76_RS23260: BWI76_RS23260 - prepilin-type cleavage/methylation domain-containing protein, at 4,851,494 to 4,851,961
_RS23260
BWI76_RS23265: BWI76_RS23265 - hypothetical protein, at 4,852,180 to 4,853,856
_RS23265
BWI76_RS23270: BWI76_RS23270 - phosphoenolpyruvate--protein phosphotransferase PtsP, at 4,854,084 to 4,856,330
_RS23270
Position (kb) 4852
4853
4854 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.399 kb on + strand, within BWI76_RS23255 at 4851.499 kb on + strand at 4851.617 kb on - strand, within BWI76_RS23260 at 4851.617 kb on - strand at 4851.642 kb on - strand, within BWI76_RS23260 at 4851.803 kb on + strand, within BWI76_RS23260 at 4851.945 kb on - strand at 4852.040 kb on - strand at 4853.023 kb on - strand, within BWI76_RS23265 at 4853.023 kb on - strand, within BWI76_RS23265 at 4854.079 kb on - strand at 4854.670 kb on + strand, within BWI76_RS23270 at 4854.740 kb on + strand, within BWI76_RS23270
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,851,397 - BWI76_RS23255 0.81 +0.9 4,851,397 - BWI76_RS23255 0.81 -2.3 4,851,399 + BWI76_RS23255 0.81 +0.6 4,851,499 + -0.1 4,851,617 - BWI76_RS23260 0.26 -0.1 4,851,617 - +2.0 4,851,642 - BWI76_RS23260 0.32 -1.4 4,851,803 + BWI76_RS23260 0.66 +0.2 4,851,945 - -0.9 4,852,040 - +0.9 4,853,023 - BWI76_RS23265 0.50 -4.7 4,853,023 - BWI76_RS23265 0.50 -3.5 4,854,079 - +0.1 4,854,670 + BWI76_RS23270 0.26 -0.4 4,854,740 + BWI76_RS23270 0.29 -1.3
Or see this region's nucleotide sequence