Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21240 and BWI76_RS21245 are separated by 91 nucleotides BWI76_RS21245 and BWI76_RS21250 are separated by 65 nucleotides BWI76_RS21250 and BWI76_RS21255 overlap by 14 nucleotides BWI76_RS21255 and BWI76_RS21260 are separated by 13 nucleotides
BWI76_RS21240: BWI76_RS21240 - flavohemoprotein, at 4,447,201 to 4,448,391
_RS21240
BWI76_RS21245: BWI76_RS21245 - nitrogen regulatory protein P-II, at 4,448,483 to 4,448,821
_RS21245
BWI76_RS21250: BWI76_RS21250 - two-component system response regulator GlrR, at 4,448,887 to 4,450,224
_RS21250
BWI76_RS21255: BWI76_RS21255 - hypothetical protein, at 4,450,211 to 4,450,915
_RS21255
BWI76_RS21260: BWI76_RS21260 - two-component sensor histidine kinase, at 4,450,929 to 4,452,383
_RS21260
Position (kb) 4448
4449
4450
4451 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4448.039 kb on - strand, within BWI76_RS21240 at 4448.094 kb on - strand, within BWI76_RS21240 at 4448.109 kb on - strand, within BWI76_RS21240 at 4448.201 kb on - strand, within BWI76_RS21240 at 4448.557 kb on - strand, within BWI76_RS21245 at 4448.578 kb on + strand, within BWI76_RS21245 at 4448.581 kb on - strand, within BWI76_RS21245 at 4448.725 kb on - strand, within BWI76_RS21245 at 4448.887 kb on - strand at 4448.892 kb on + strand at 4448.989 kb on - strand at 4449.382 kb on - strand, within BWI76_RS21250 at 4449.451 kb on + strand, within BWI76_RS21250 at 4449.855 kb on + strand at 4449.875 kb on + strand, within BWI76_RS21250 at 4449.883 kb on - strand, within BWI76_RS21250 at 4449.962 kb on - strand, within BWI76_RS21250 at 4449.962 kb on - strand, within BWI76_RS21250 at 4450.023 kb on + strand, within BWI76_RS21250 at 4450.055 kb on + strand, within BWI76_RS21250 at 4450.420 kb on - strand, within BWI76_RS21255 at 4450.468 kb on - strand, within BWI76_RS21255 at 4450.473 kb on + strand, within BWI76_RS21255 at 4450.481 kb on - strand at 4450.524 kb on - strand, within BWI76_RS21255 at 4450.689 kb on + strand, within BWI76_RS21255 at 4451.054 kb on + strand at 4451.054 kb on + strand at 4451.054 kb on + strand at 4451.062 kb on - strand at 4451.088 kb on - strand, within BWI76_RS21260
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,448,039 - BWI76_RS21240 0.70 +0.7 4,448,094 - BWI76_RS21240 0.75 +0.0 4,448,109 - BWI76_RS21240 0.76 -0.0 4,448,201 - BWI76_RS21240 0.84 +0.2 4,448,557 - BWI76_RS21245 0.22 -1.9 4,448,578 + BWI76_RS21245 0.28 -0.7 4,448,581 - BWI76_RS21245 0.29 -0.0 4,448,725 - BWI76_RS21245 0.71 -0.7 4,448,887 - -0.1 4,448,892 + -1.2 4,448,989 - -0.8 4,449,382 - BWI76_RS21250 0.37 -0.5 4,449,451 + BWI76_RS21250 0.42 -2.6 4,449,855 + -0.1 4,449,875 + BWI76_RS21250 0.74 -1.7 4,449,883 - BWI76_RS21250 0.74 -0.8 4,449,962 - BWI76_RS21250 0.80 -0.9 4,449,962 - BWI76_RS21250 0.80 -0.8 4,450,023 + BWI76_RS21250 0.85 -0.8 4,450,055 + BWI76_RS21250 0.87 -2.0 4,450,420 - BWI76_RS21255 0.30 -0.5 4,450,468 - BWI76_RS21255 0.36 -0.6 4,450,473 + BWI76_RS21255 0.37 -1.1 4,450,481 - -0.7 4,450,524 - BWI76_RS21255 0.44 -3.1 4,450,689 + BWI76_RS21255 0.68 -0.3 4,451,054 + +0.4 4,451,054 + -2.6 4,451,054 + -0.4 4,451,062 - -0.1 4,451,088 - BWI76_RS21260 0.11 -0.7
Or see this region's nucleotide sequence