Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20180 and BWI76_RS20185 are separated by 98 nucleotides BWI76_RS20185 and BWI76_RS20190 are separated by 15 nucleotides BWI76_RS20190 and BWI76_RS20195 overlap by 23 nucleotides BWI76_RS20195 and BWI76_RS20200 are separated by 463 nucleotides
BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763
_RS20180
BWI76_RS20185: BWI76_RS20185 - NADH-quinone oxidoreductase subunit B, at 4,230,862 to 4,231,536
_RS20185
BWI76_RS20190: BWI76_RS20190 - NADH-quinone oxidoreductase subunit A, at 4,231,552 to 4,231,998
_RS20190
BWI76_RS20195: BWI76_RS20195 - hypothetical protein, at 4,231,976 to 4,232,186
_RS20195
BWI76_RS20200: BWI76_RS20200 - transcriptional regulator LrhA, at 4,232,650 to 4,233,576
_RS20200
Position (kb) 4231
4232 Strain fitness (log2 ratio) -1
0
1 at 4230.870 kb on + strand at 4231.087 kb on + strand, within BWI76_RS20185 at 4231.302 kb on - strand at 4231.557 kb on + strand at 4231.565 kb on - strand at 4231.591 kb on - strand at 4231.657 kb on - strand, within BWI76_RS20190 at 4231.908 kb on + strand, within BWI76_RS20190 at 4231.926 kb on - strand, within BWI76_RS20190 at 4232.281 kb on - strand at 4232.334 kb on - strand at 4232.434 kb on - strand at 4232.691 kb on - strand at 4232.705 kb on - strand at 4232.874 kb on + strand, within BWI76_RS20200 at 4232.962 kb on - strand, within BWI76_RS20200 at 4232.962 kb on - strand, within BWI76_RS20200
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,230,870 + +0.9 4,231,087 + BWI76_RS20185 0.33 +0.9 4,231,302 - -0.7 4,231,557 + +0.1 4,231,565 - -0.8 4,231,591 - +0.3 4,231,657 - BWI76_RS20190 0.23 -1.1 4,231,908 + BWI76_RS20190 0.80 -0.8 4,231,926 - BWI76_RS20190 0.84 -0.9 4,232,281 - -1.3 4,232,334 - +0.9 4,232,434 - +0.6 4,232,691 - +0.1 4,232,705 - +0.1 4,232,874 + BWI76_RS20200 0.24 +0.4 4,232,962 - BWI76_RS20200 0.34 +0.2 4,232,962 - BWI76_RS20200 0.34 +0.3
Or see this region's nucleotide sequence