Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20125 and BWI76_RS20130 are separated by 202 nucleotides BWI76_RS20130 and BWI76_RS20135 are separated by 6 nucleotides
BWI76_RS20125: BWI76_RS20125 - integrase, at 4,216,233 to 4,216,784
_RS20125
BWI76_RS20130: BWI76_RS20130 - NADH-quinone oxidoreductase subunit N, at 4,216,987 to 4,218,444
_RS20130
BWI76_RS20135: BWI76_RS20135 - NADH-quinone oxidoreductase subunit M, at 4,218,451 to 4,219,980
_RS20135
Position (kb) 4216
4217
4218
4219 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4216.028 kb on - strand at 4216.073 kb on - strand at 4216.074 kb on - strand at 4216.336 kb on + strand, within BWI76_RS20125 at 4216.357 kb on - strand, within BWI76_RS20125 at 4216.449 kb on + strand, within BWI76_RS20125 at 4216.529 kb on + strand, within BWI76_RS20125 at 4216.538 kb on + strand, within BWI76_RS20125 at 4216.539 kb on - strand, within BWI76_RS20125 at 4216.545 kb on - strand, within BWI76_RS20125 at 4216.546 kb on - strand, within BWI76_RS20125 at 4216.666 kb on - strand, within BWI76_RS20125 at 4216.719 kb on + strand, within BWI76_RS20125 at 4216.719 kb on + strand, within BWI76_RS20125 at 4216.723 kb on - strand, within BWI76_RS20125 at 4216.727 kb on - strand, within BWI76_RS20125 at 4216.728 kb on - strand, within BWI76_RS20125 at 4216.740 kb on + strand at 4216.741 kb on + strand at 4216.798 kb on + strand at 4216.798 kb on + strand at 4216.806 kb on - strand at 4216.808 kb on + strand at 4216.811 kb on - strand at 4216.958 kb on - strand at 4216.983 kb on - strand at 4216.990 kb on + strand at 4216.993 kb on - strand at 4216.993 kb on - strand at 4217.006 kb on - strand at 4217.010 kb on + strand at 4217.010 kb on + strand at 4217.015 kb on + strand at 4217.015 kb on + strand at 4217.020 kb on + strand at 4217.028 kb on - strand at 4217.042 kb on + strand at 4217.046 kb on + strand at 4217.050 kb on - strand at 4217.050 kb on - strand at 4217.090 kb on + strand at 4217.145 kb on + strand, within BWI76_RS20130 at 4217.145 kb on + strand, within BWI76_RS20130 at 4217.160 kb on - strand, within BWI76_RS20130 at 4217.427 kb on + strand, within BWI76_RS20130 at 4217.435 kb on - strand, within BWI76_RS20130 at 4217.909 kb on + strand, within BWI76_RS20130 at 4218.271 kb on + strand, within BWI76_RS20130 at 4218.731 kb on + strand, within BWI76_RS20135 at 4219.060 kb on + strand, within BWI76_RS20135
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,216,028 - -0.7 4,216,073 - +0.9 4,216,074 - +0.9 4,216,336 + BWI76_RS20125 0.19 -0.3 4,216,357 - BWI76_RS20125 0.22 +0.9 4,216,449 + BWI76_RS20125 0.39 -0.9 4,216,529 + BWI76_RS20125 0.54 +1.2 4,216,538 + BWI76_RS20125 0.55 +0.3 4,216,539 - BWI76_RS20125 0.55 -0.3 4,216,545 - BWI76_RS20125 0.57 -2.6 4,216,546 - BWI76_RS20125 0.57 -0.9 4,216,666 - BWI76_RS20125 0.78 +0.1 4,216,719 + BWI76_RS20125 0.88 -2.1 4,216,719 + BWI76_RS20125 0.88 -0.7 4,216,723 - BWI76_RS20125 0.89 -1.1 4,216,727 - BWI76_RS20125 0.89 -0.7 4,216,728 - BWI76_RS20125 0.90 +0.1 4,216,740 + -2.3 4,216,741 + -0.4 4,216,798 + -1.7 4,216,798 + +0.5 4,216,806 - +0.3 4,216,808 + -3.0 4,216,811 - -0.1 4,216,958 - +0.9 4,216,983 - -0.1 4,216,990 + -1.6 4,216,993 - -0.9 4,216,993 - -0.3 4,217,006 - -1.9 4,217,010 + -1.7 4,217,010 + -0.1 4,217,015 + -2.1 4,217,015 + -0.5 4,217,020 + +1.9 4,217,028 - +0.4 4,217,042 + -2.4 4,217,046 + -0.7 4,217,050 - -0.8 4,217,050 - -1.4 4,217,090 + -2.1 4,217,145 + BWI76_RS20130 0.11 -2.1 4,217,145 + BWI76_RS20130 0.11 -0.8 4,217,160 - BWI76_RS20130 0.12 -0.2 4,217,427 + BWI76_RS20130 0.30 -1.3 4,217,435 - BWI76_RS20130 0.31 -1.0 4,217,909 + BWI76_RS20130 0.63 -2.1 4,218,271 + BWI76_RS20130 0.88 -0.7 4,218,731 + BWI76_RS20135 0.18 +0.0 4,219,060 + BWI76_RS20135 0.40 -1.2
Or see this region's nucleotide sequence