Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08350 and BWI76_RS08355 overlap by 7 nucleotides BWI76_RS08355 and BWI76_RS08360 overlap by 11 nucleotides BWI76_RS08360 and BWI76_RS08365 are separated by 62 nucleotides BWI76_RS08365 and BWI76_RS08370 overlap by 4 nucleotides
BWI76_RS08350: BWI76_RS08350 - carboxylase, at 1,768,073 to 1,768,729
_RS08350
BWI76_RS08355: BWI76_RS08355 - hypothetical protein, at 1,768,723 to 1,769,655
_RS08355
BWI76_RS08360: BWI76_RS08360 - LamB/YcsF family protein, at 1,769,645 to 1,770,391
_RS08360
BWI76_RS08365: BWI76_RS08365 - membrane protein, at 1,770,454 to 1,771,179
_RS08365
BWI76_RS08370: BWI76_RS08370 - membrane protein, at 1,771,176 to 1,772,171
_RS08370
Position (kb) 1769
1770
1771 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1768.767 kb on - strand at 1768.889 kb on + strand, within BWI76_RS08355 at 1768.985 kb on - strand, within BWI76_RS08355 at 1769.103 kb on + strand, within BWI76_RS08355 at 1769.213 kb on + strand, within BWI76_RS08355 at 1769.221 kb on - strand, within BWI76_RS08355 at 1769.373 kb on - strand, within BWI76_RS08355 at 1769.610 kb on + strand at 1769.791 kb on - strand, within BWI76_RS08360 at 1770.049 kb on - strand, within BWI76_RS08360 at 1770.217 kb on - strand, within BWI76_RS08360 at 1770.298 kb on + strand, within BWI76_RS08360 at 1770.711 kb on + strand, within BWI76_RS08365 at 1771.143 kb on + strand at 1771.145 kb on - strand at 1771.151 kb on - strand at 1771.234 kb on - strand at 1771.286 kb on + strand, within BWI76_RS08370 at 1771.330 kb on + strand, within BWI76_RS08370
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,768,767 - +0.7 1,768,889 + BWI76_RS08355 0.18 +0.9 1,768,985 - BWI76_RS08355 0.28 +0.3 1,769,103 + BWI76_RS08355 0.41 -0.1 1,769,213 + BWI76_RS08355 0.53 -0.5 1,769,221 - BWI76_RS08355 0.53 -0.3 1,769,373 - BWI76_RS08355 0.70 +0.2 1,769,610 + -0.5 1,769,791 - BWI76_RS08360 0.20 -0.9 1,770,049 - BWI76_RS08360 0.54 -3.1 1,770,217 - BWI76_RS08360 0.77 -0.4 1,770,298 + BWI76_RS08360 0.87 -0.1 1,770,711 + BWI76_RS08365 0.35 +0.4 1,771,143 + +0.4 1,771,145 - +0.5 1,771,151 - +0.4 1,771,234 - +2.3 1,771,286 + BWI76_RS08370 0.11 +0.2 1,771,330 + BWI76_RS08370 0.15 -1.1
Or see this region's nucleotide sequence