Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06530 and BWI76_RS06535 are separated by 183 nucleotides BWI76_RS06535 and BWI76_RS06540 are separated by 208 nucleotides BWI76_RS06540 and BWI76_RS06545 are separated by 213 nucleotides
BWI76_RS06530: BWI76_RS06530 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 1,371,243 to 1,372,517
_RS06530
BWI76_RS06535: BWI76_RS06535 - endopeptidase La, at 1,372,701 to 1,375,055
_RS06535
BWI76_RS06540: BWI76_RS06540 - DNA-binding protein HU-beta, at 1,375,264 to 1,375,536
_RS06540
BWI76_RS06545: BWI76_RS06545 - peptidylprolyl isomerase, at 1,375,750 to 1,377,624
_RS06545
Position (kb) 1372
1373
1374
1375
1376 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1371.705 kb on + strand, within BWI76_RS06530 at 1371.748 kb on - strand, within BWI76_RS06530 at 1372.140 kb on - strand, within BWI76_RS06530 at 1372.382 kb on + strand, within BWI76_RS06530 at 1372.590 kb on + strand at 1372.827 kb on + strand at 1372.968 kb on + strand, within BWI76_RS06535 at 1373.007 kb on - strand, within BWI76_RS06535 at 1373.007 kb on - strand, within BWI76_RS06535 at 1373.986 kb on + strand, within BWI76_RS06535 at 1374.121 kb on - strand, within BWI76_RS06535 at 1374.267 kb on + strand, within BWI76_RS06535 at 1374.486 kb on + strand, within BWI76_RS06535 at 1374.486 kb on + strand, within BWI76_RS06535 at 1374.890 kb on + strand at 1374.898 kb on - strand at 1375.143 kb on - strand at 1375.165 kb on + strand at 1375.166 kb on - strand at 1375.170 kb on + strand at 1375.173 kb on - strand at 1375.177 kb on + strand at 1375.199 kb on + strand at 1375.222 kb on + strand at 1375.425 kb on - strand, within BWI76_RS06540 at 1375.507 kb on + strand, within BWI76_RS06540 at 1375.636 kb on + strand at 1375.636 kb on + strand at 1375.836 kb on + strand at 1376.020 kb on + strand, within BWI76_RS06545
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,371,705 + BWI76_RS06530 0.36 -0.1 1,371,748 - BWI76_RS06530 0.40 -0.5 1,372,140 - BWI76_RS06530 0.70 +3.7 1,372,382 + BWI76_RS06530 0.89 -0.1 1,372,590 + -0.8 1,372,827 + -3.4 1,372,968 + BWI76_RS06535 0.11 -2.3 1,373,007 - BWI76_RS06535 0.13 -0.1 1,373,007 - BWI76_RS06535 0.13 -2.3 1,373,986 + BWI76_RS06535 0.55 -0.1 1,374,121 - BWI76_RS06535 0.60 -0.7 1,374,267 + BWI76_RS06535 0.66 +0.3 1,374,486 + BWI76_RS06535 0.76 -3.5 1,374,486 + BWI76_RS06535 0.76 -1.1 1,374,890 + -0.7 1,374,898 - -1.1 1,375,143 - +1.4 1,375,165 + -0.2 1,375,166 - +1.1 1,375,170 + +0.6 1,375,173 - -0.4 1,375,177 + +0.2 1,375,199 + -0.4 1,375,222 + +0.4 1,375,425 - BWI76_RS06540 0.59 +3.2 1,375,507 + BWI76_RS06540 0.89 -0.6 1,375,636 + +0.7 1,375,636 + +0.9 1,375,836 + -0.0 1,376,020 + BWI76_RS06545 0.14 -3.1
Or see this region's nucleotide sequence