Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03115

Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03110 and BWI76_RS03115 are separated by 172 nucleotidesBWI76_RS03115 and BWI76_RS03120 are separated by 139 nucleotides BWI76_RS03110: BWI76_RS03110 - MFS transporter, at 640,200 to 641,699 _RS03110 BWI76_RS03115: BWI76_RS03115 - ABC transporter substrate-binding protein, at 641,872 to 643,182 _RS03115 BWI76_RS03120: BWI76_RS03120 - ABC transporter ATP-binding protein, at 643,322 to 644,392 _RS03120 Position (kb) 641 642 643 644Strain fitness (log2 ratio) -1 0 1 2 3at 640.894 kb on - strand, within BWI76_RS03110at 640.981 kb on - strand, within BWI76_RS03110at 641.008 kb on - strand, within BWI76_RS03110at 641.063 kb on - strand, within BWI76_RS03110at 641.076 kb on + strand, within BWI76_RS03110at 641.076 kb on + strand, within BWI76_RS03110at 641.076 kb on + strand, within BWI76_RS03110at 641.084 kb on - strand, within BWI76_RS03110at 641.153 kb on - strand, within BWI76_RS03110at 641.313 kb on - strand, within BWI76_RS03110at 641.494 kb on + strand, within BWI76_RS03110at 641.585 kb on + strandat 641.590 kb on + strandat 641.694 kb on + strandat 641.702 kb on - strandat 641.702 kb on - strandat 641.702 kb on - strandat 641.702 kb on - strandat 641.724 kb on + strandat 641.802 kb on + strandat 642.018 kb on + strand, within BWI76_RS03115at 642.064 kb on - strand, within BWI76_RS03115at 642.075 kb on + strand, within BWI76_RS03115at 642.093 kb on - strand, within BWI76_RS03115at 642.134 kb on + strand, within BWI76_RS03115at 642.246 kb on + strand, within BWI76_RS03115at 642.254 kb on - strand, within BWI76_RS03115at 642.264 kb on - strand, within BWI76_RS03115at 642.365 kb on + strand, within BWI76_RS03115at 642.570 kb on - strand, within BWI76_RS03115at 642.648 kb on + strand, within BWI76_RS03115at 642.687 kb on - strand, within BWI76_RS03115at 642.773 kb on - strand, within BWI76_RS03115at 642.859 kb on - strand, within BWI76_RS03115at 642.867 kb on - strand, within BWI76_RS03115at 642.984 kb on + strand, within BWI76_RS03115at 643.062 kb on - strandat 643.079 kb on - strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.149 kb on + strandat 643.237 kb on + strandat 643.237 kb on + strandat 643.245 kb on - strandat 643.458 kb on - strand, within BWI76_RS03120at 643.468 kb on - strand, within BWI76_RS03120at 643.562 kb on + strand, within BWI76_RS03120at 643.628 kb on + strand, within BWI76_RS03120at 643.736 kb on + strand, within BWI76_RS03120at 643.744 kb on - strand, within BWI76_RS03120at 643.827 kb on - strand, within BWI76_RS03120at 644.049 kb on + strand, within BWI76_RS03120

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
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640,894 - BWI76_RS03110 0.46 +0.4
640,981 - BWI76_RS03110 0.52 +0.8
641,008 - BWI76_RS03110 0.54 -0.2
641,063 - BWI76_RS03110 0.58 -0.2
641,076 + BWI76_RS03110 0.58 -0.2
641,076 + BWI76_RS03110 0.58 +0.6
641,076 + BWI76_RS03110 0.58 +0.2
641,084 - BWI76_RS03110 0.59 +0.1
641,153 - BWI76_RS03110 0.64 +1.3
641,313 - BWI76_RS03110 0.74 +0.7
641,494 + BWI76_RS03110 0.86 -1.4
641,585 + +2.6
641,590 + +2.6
641,694 + +1.1
641,702 - +0.1
641,702 - +0.3
641,702 - -1.1
641,702 - +0.4
641,724 + +1.0
641,802 + -0.2
642,018 + BWI76_RS03115 0.11 +0.6
642,064 - BWI76_RS03115 0.15 -0.0
642,075 + BWI76_RS03115 0.15 +0.6
642,093 - BWI76_RS03115 0.17 +0.3
642,134 + BWI76_RS03115 0.20 +1.4
642,246 + BWI76_RS03115 0.29 +0.2
642,254 - BWI76_RS03115 0.29 -0.6
642,264 - BWI76_RS03115 0.30 +0.4
642,365 + BWI76_RS03115 0.38 +1.0
642,570 - BWI76_RS03115 0.53 -0.9
642,648 + BWI76_RS03115 0.59 +1.0
642,687 - BWI76_RS03115 0.62 +0.2
642,773 - BWI76_RS03115 0.69 +1.1
642,859 - BWI76_RS03115 0.75 +1.1
642,867 - BWI76_RS03115 0.76 +0.8
642,984 + BWI76_RS03115 0.85 +0.3
643,062 - -0.0
643,079 - -0.0
643,137 + -0.0
643,137 + -0.6
643,137 + -0.2
643,137 + -1.0
643,137 + -0.1
643,145 - +0.3
643,145 - -0.6
643,145 - +0.5
643,145 - +0.6
643,145 - +0.1
643,149 + +0.6
643,237 + +0.4
643,237 + +3.3
643,245 - -0.3
643,458 - BWI76_RS03120 0.13 +0.4
643,468 - BWI76_RS03120 0.14 +0.4
643,562 + BWI76_RS03120 0.22 -0.0
643,628 + BWI76_RS03120 0.29 -1.2
643,736 + BWI76_RS03120 0.39 -0.0
643,744 - BWI76_RS03120 0.39 -0.1
643,827 - BWI76_RS03120 0.47 +0.1
644,049 + BWI76_RS03120 0.68 -0.6

Or see this region's nucleotide sequence