Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotides BWI76_RS01110 and BWI76_RS01115 are separated by 4 nucleotides BWI76_RS01115 and BWI76_RS01120 are separated by 1 nucleotides
BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787
_RS01105
BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439
_RS01110
BWI76_RS01115: BWI76_RS01115 - dTDP-4-amino-4,6-dideoxygalactose transaminase, at 229,444 to 230,574
_RS01115
BWI76_RS01120: BWI76_RS01120 - O-antigen translocase, at 230,576 to 231,826
_RS01120
Position (kb) 229
230
231 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 228.654 kb on + strand, within BWI76_RS01105 at 228.789 kb on - strand at 228.839 kb on - strand, within BWI76_RS01110 at 228.927 kb on + strand, within BWI76_RS01110 at 229.276 kb on - strand, within BWI76_RS01110 at 229.279 kb on + strand, within BWI76_RS01110 at 229.315 kb on + strand, within BWI76_RS01110 at 229.536 kb on - strand at 229.602 kb on + strand, within BWI76_RS01115 at 229.711 kb on - strand, within BWI76_RS01115 at 229.716 kb on - strand, within BWI76_RS01115 at 230.473 kb on + strand at 230.481 kb on - strand at 230.528 kb on - strand at 230.592 kb on - strand at 230.926 kb on - strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 231.208 kb on + strand, within BWI76_RS01120 at 231.388 kb on + strand, within BWI76_RS01120 at 231.410 kb on + strand, within BWI76_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 228,654 + BWI76_RS01105 0.85 -1.3 228,789 - -1.9 228,839 - BWI76_RS01110 0.11 -2.9 228,927 + BWI76_RS01110 0.24 -2.0 229,276 - BWI76_RS01110 0.76 -1.6 229,279 + BWI76_RS01110 0.76 -1.4 229,315 + BWI76_RS01110 0.81 -1.5 229,536 - -4.1 229,602 + BWI76_RS01115 0.14 -2.8 229,711 - BWI76_RS01115 0.24 -1.0 229,716 - BWI76_RS01115 0.24 -2.7 230,473 + -0.6 230,481 - +0.0 230,528 - -3.6 230,592 - +0.0 230,926 - BWI76_RS01120 0.28 -2.7 230,953 + BWI76_RS01120 0.30 +0.3 230,953 + BWI76_RS01120 0.30 +0.2 231,208 + BWI76_RS01120 0.51 -0.1 231,388 + BWI76_RS01120 0.65 +0.5 231,410 + BWI76_RS01120 0.67 -0.9
Or see this region's nucleotide sequence