Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_949 and Pf6N2E2_950 are separated by 516 nucleotides Pf6N2E2_950 and Pf6N2E2_951 overlap by 1 nucleotides Pf6N2E2_951 and Pf6N2E2_952 are separated by 70 nucleotides
Pf6N2E2_949 - Outer membrane protein OprN, at 1,118,637 to 1,120,055
_949
Pf6N2E2_950 - Type IV secretory pathway, VirB4 components, at 1,120,572 to 1,123,322
_950
Pf6N2E2_951 - putative lipoprotein, at 1,123,322 to 1,123,630
_951
Pf6N2E2_952 - putative exported protein, at 1,123,701 to 1,125,215
_952
Position (kb)
1120
1121
1122
1123
1124 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1119.659 kb on + strand, within Pf6N2E2_949 at 1119.659 kb on + strand, within Pf6N2E2_949 at 1119.660 kb on - strand, within Pf6N2E2_949 at 1119.741 kb on + strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.879 kb on + strand, within Pf6N2E2_949 at 1119.880 kb on - strand, within Pf6N2E2_949 at 1119.900 kb on + strand, within Pf6N2E2_949 at 1119.901 kb on - strand, within Pf6N2E2_949 at 1119.996 kb on + strand at 1119.997 kb on - strand at 1119.997 kb on - strand at 1119.998 kb on + strand at 1119.999 kb on - strand at 1119.999 kb on - strand at 1120.091 kb on + strand at 1120.112 kb on + strand at 1120.210 kb on - strand at 1120.215 kb on + strand at 1120.216 kb on - strand at 1120.303 kb on + strand at 1120.352 kb on + strand at 1120.353 kb on - strand at 1120.449 kb on + strand at 1120.449 kb on + strand at 1120.450 kb on - strand at 1120.550 kb on + strand at 1120.573 kb on + strand at 1120.573 kb on + strand at 1120.708 kb on + strand at 1120.774 kb on + strand at 1120.774 kb on + strand at 1120.774 kb on + strand at 1121.123 kb on - strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.162 kb on - strand, within Pf6N2E2_950 at 1121.162 kb on - strand, within Pf6N2E2_950 at 1121.162 kb on - strand, within Pf6N2E2_950 at 1121.652 kb on - strand, within Pf6N2E2_950 at 1121.681 kb on - strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1122.001 kb on + strand, within Pf6N2E2_950 at 1122.002 kb on - strand, within Pf6N2E2_950 at 1122.145 kb on + strand, within Pf6N2E2_950 at 1122.145 kb on + strand, within Pf6N2E2_950 at 1122.167 kb on - strand, within Pf6N2E2_950 at 1122.285 kb on + strand, within Pf6N2E2_950 at 1122.285 kb on + strand, within Pf6N2E2_950 at 1122.286 kb on - strand, within Pf6N2E2_950 at 1122.286 kb on - strand, within Pf6N2E2_950 at 1122.326 kb on + strand, within Pf6N2E2_950 at 1122.327 kb on - strand, within Pf6N2E2_950 at 1122.580 kb on + strand, within Pf6N2E2_950 at 1122.581 kb on - strand, within Pf6N2E2_950 at 1122.646 kb on + strand, within Pf6N2E2_950 at 1122.646 kb on + strand, within Pf6N2E2_950 at 1122.782 kb on - strand, within Pf6N2E2_950 at 1122.901 kb on + strand, within Pf6N2E2_950 at 1122.901 kb on + strand, within Pf6N2E2_950 at 1122.901 kb on + strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.944 kb on - strand, within Pf6N2E2_950 at 1122.964 kb on + strand, within Pf6N2E2_950 at 1123.181 kb on - strand at 1123.225 kb on + strand at 1123.225 kb on + strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.323 kb on + strand at 1123.323 kb on + strand at 1123.323 kb on + strand at 1123.324 kb on - strand at 1123.357 kb on - strand, within Pf6N2E2_951 at 1123.371 kb on + strand, within Pf6N2E2_951 at 1123.372 kb on - strand, within Pf6N2E2_951 at 1123.434 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.437 kb on - strand, within Pf6N2E2_951 at 1123.437 kb on - strand, within Pf6N2E2_951 at 1123.437 kb on - strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.441 kb on - strand, within Pf6N2E2_951 at 1123.441 kb on - strand, within Pf6N2E2_951 at 1123.441 kb on - strand, within Pf6N2E2_951 at 1123.538 kb on + strand, within Pf6N2E2_951 at 1123.538 kb on + strand, within Pf6N2E2_951 at 1123.539 kb on - strand, within Pf6N2E2_951 at 1123.559 kb on + strand, within Pf6N2E2_951 at 1123.559 kb on + strand, within Pf6N2E2_951 at 1123.822 kb on + strand at 1123.822 kb on + strand at 1123.823 kb on - strand at 1123.823 kb on - strand at 1123.846 kb on + strand at 1123.847 kb on - strand at 1124.041 kb on + strand, within Pf6N2E2_952 at 1124.041 kb on + strand, within Pf6N2E2_952 at 1124.192 kb on + strand, within Pf6N2E2_952 at 1124.192 kb on + strand, within Pf6N2E2_952
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 1,119,659 + Pf6N2E2_949 0.72 +1.0 1,119,659 + Pf6N2E2_949 0.72 -0.4 1,119,660 - Pf6N2E2_949 0.72 +0.6 1,119,741 + Pf6N2E2_949 0.78 +1.7 1,119,742 - Pf6N2E2_949 0.78 +0.6 1,119,742 - Pf6N2E2_949 0.78 +0.2 1,119,742 - Pf6N2E2_949 0.78 +0.8 1,119,742 - Pf6N2E2_949 0.78 +1.0 1,119,879 + Pf6N2E2_949 0.88 +0.7 1,119,880 - Pf6N2E2_949 0.88 +0.4 1,119,900 + Pf6N2E2_949 0.89 -0.1 1,119,901 - Pf6N2E2_949 0.89 -0.3 1,119,996 + -0.3 1,119,997 - -0.4 1,119,997 - +0.0 1,119,998 + -0.6 1,119,999 - -0.1 1,119,999 - -1.7 1,120,091 + -0.1 1,120,112 + +2.9 1,120,210 - -0.7 1,120,215 + -0.7 1,120,216 - -1.4 1,120,303 + -0.1 1,120,352 + +0.6 1,120,353 - -0.4 1,120,449 + +0.6 1,120,449 + +1.2 1,120,450 - -0.4 1,120,550 + +2.2 1,120,573 + +0.6 1,120,573 + -0.4 1,120,708 + -0.8 1,120,774 + -0.7 1,120,774 + +0.0 1,120,774 + +0.6 1,121,123 - Pf6N2E2_950 0.20 -0.4 1,121,161 + Pf6N2E2_950 0.21 -1.4 1,121,161 + Pf6N2E2_950 0.21 +0.1 1,121,161 + Pf6N2E2_950 0.21 +0.6 1,121,161 + Pf6N2E2_950 0.21 -1.1 1,121,161 + Pf6N2E2_950 0.21 -0.1 1,121,162 - Pf6N2E2_950 0.21 -1.0 1,121,162 - Pf6N2E2_950 0.21 -1.4 1,121,162 - Pf6N2E2_950 0.21 +0.6 1,121,652 - Pf6N2E2_950 0.39 -0.4 1,121,681 - Pf6N2E2_950 0.40 +1.2 1,121,724 + Pf6N2E2_950 0.42 -0.2 1,121,724 + Pf6N2E2_950 0.42 -0.3 1,121,724 + Pf6N2E2_950 0.42 -2.2 1,121,724 + Pf6N2E2_950 0.42 -0.4 1,121,724 + Pf6N2E2_950 0.42 -0.1 1,121,724 + Pf6N2E2_950 0.42 -1.8 1,121,725 - Pf6N2E2_950 0.42 -0.4 1,121,725 - Pf6N2E2_950 0.42 -0.4 1,121,725 - Pf6N2E2_950 0.42 +0.9 1,121,725 - Pf6N2E2_950 0.42 -0.4 1,122,001 + Pf6N2E2_950 0.52 +0.0 1,122,002 - Pf6N2E2_950 0.52 -0.3 1,122,145 + Pf6N2E2_950 0.57 +0.3 1,122,145 + Pf6N2E2_950 0.57 +0.8 1,122,167 - Pf6N2E2_950 0.58 +0.3 1,122,285 + Pf6N2E2_950 0.62 -0.6 1,122,285 + Pf6N2E2_950 0.62 +0.1 1,122,286 - Pf6N2E2_950 0.62 -0.4 1,122,286 - Pf6N2E2_950 0.62 +0.6 1,122,326 + Pf6N2E2_950 0.64 -0.4 1,122,327 - Pf6N2E2_950 0.64 +0.1 1,122,580 + Pf6N2E2_950 0.73 +0.2 1,122,581 - Pf6N2E2_950 0.73 -0.2 1,122,646 + Pf6N2E2_950 0.75 +1.3 1,122,646 + Pf6N2E2_950 0.75 +0.6 1,122,782 - Pf6N2E2_950 0.80 -0.7 1,122,901 + Pf6N2E2_950 0.85 -1.0 1,122,901 + Pf6N2E2_950 0.85 -0.1 1,122,901 + Pf6N2E2_950 0.85 -1.0 1,122,902 - Pf6N2E2_950 0.85 +1.0 1,122,902 - Pf6N2E2_950 0.85 +0.1 1,122,902 - Pf6N2E2_950 0.85 +0.0 1,122,902 - Pf6N2E2_950 0.85 +1.0 1,122,902 - Pf6N2E2_950 0.85 -0.7 1,122,943 + Pf6N2E2_950 0.86 -1.4 1,122,943 + Pf6N2E2_950 0.86 +0.6 1,122,943 + Pf6N2E2_950 0.86 +0.3 1,122,943 + Pf6N2E2_950 0.86 -1.4 1,122,944 - Pf6N2E2_950 0.86 -0.4 1,122,964 + Pf6N2E2_950 0.87 +0.3 1,123,181 - +0.4 1,123,225 + +0.8 1,123,225 + +0.2 1,123,226 - -1.3 1,123,226 - +0.9 1,123,226 - +0.0 1,123,226 - -1.6 1,123,226 - +0.6 1,123,323 + +0.1 1,123,323 + -0.1 1,123,323 + +0.2 1,123,324 - +1.2 1,123,357 - Pf6N2E2_951 0.11 +0.6 1,123,371 + Pf6N2E2_951 0.16 -0.6 1,123,372 - Pf6N2E2_951 0.16 +0.4 1,123,434 + Pf6N2E2_951 0.36 -0.7 1,123,436 + Pf6N2E2_951 0.37 +2.2 1,123,436 + Pf6N2E2_951 0.37 +0.4 1,123,436 + Pf6N2E2_951 0.37 -0.8 1,123,436 + Pf6N2E2_951 0.37 +1.6 1,123,436 + Pf6N2E2_951 0.37 +1.0 1,123,436 + Pf6N2E2_951 0.37 +0.6 1,123,436 + Pf6N2E2_951 0.37 -0.4 1,123,436 + Pf6N2E2_951 0.37 -0.4 1,123,436 + Pf6N2E2_951 0.37 +0.1 1,123,436 + Pf6N2E2_951 0.37 -1.4 1,123,436 + Pf6N2E2_951 0.37 -0.4 1,123,437 - Pf6N2E2_951 0.37 -0.2 1,123,437 - Pf6N2E2_951 0.37 +0.6 1,123,437 - Pf6N2E2_951 0.37 +2.2 1,123,440 + Pf6N2E2_951 0.38 +0.2 1,123,440 + Pf6N2E2_951 0.38 +0.1 1,123,440 + Pf6N2E2_951 0.38 -1.4 1,123,440 + Pf6N2E2_951 0.38 -0.2 1,123,440 + Pf6N2E2_951 0.38 +1.6 1,123,441 - Pf6N2E2_951 0.39 -1.7 1,123,441 - Pf6N2E2_951 0.39 -0.7 1,123,441 - Pf6N2E2_951 0.39 +0.6 1,123,538 + Pf6N2E2_951 0.70 +0.2 1,123,538 + Pf6N2E2_951 0.70 +1.6 1,123,539 - Pf6N2E2_951 0.70 +0.6 1,123,559 + Pf6N2E2_951 0.77 -0.4 1,123,559 + Pf6N2E2_951 0.77 +0.4 1,123,822 + -1.0 1,123,822 + +1.6 1,123,823 - +0.9 1,123,823 - -1.1 1,123,846 + +0.6 1,123,847 - -0.5 1,124,041 + Pf6N2E2_952 0.22 +0.6 1,124,041 + Pf6N2E2_952 0.22 +1.6 1,124,192 + Pf6N2E2_952 0.32 -0.4 1,124,192 + Pf6N2E2_952 0.32 +0.6
Or see this region's nucleotide sequence