Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1435 and Pf6N2E2_1436 are separated by 40 nucleotides Pf6N2E2_1436 and Pf6N2E2_1437 are separated by 377 nucleotides
Pf6N2E2_1435 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase, at 1,684,697 to 1,685,794
_1435
Pf6N2E2_1436 - FMN reductase (EC 1.5.1.29), at 1,685,835 to 1,686,398
_1436
Pf6N2E2_1437 - Acyl-CoA dehydrogenase family protein, at 1,686,776 to 1,688,020
_1437
Position (kb)
1685
1686
1687 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1685.163 kb on - strand, within Pf6N2E2_1435 at 1685.185 kb on - strand, within Pf6N2E2_1435 at 1685.284 kb on - strand, within Pf6N2E2_1435 at 1685.649 kb on + strand, within Pf6N2E2_1435 at 1685.694 kb on + strand at 1685.694 kb on + strand at 1685.695 kb on - strand at 1685.695 kb on - strand at 1685.766 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.801 kb on + strand at 1685.802 kb on - strand at 1685.802 kb on - strand at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1686.079 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.149 kb on - strand, within Pf6N2E2_1436 at 1686.447 kb on - strand at 1686.483 kb on - strand at 1687.254 kb on - strand, within Pf6N2E2_1437 at 1687.256 kb on + strand, within Pf6N2E2_1437 at 1687.256 kb on + strand, within Pf6N2E2_1437 at 1687.257 kb on - strand, within Pf6N2E2_1437 at 1687.270 kb on + strand, within Pf6N2E2_1437 at 1687.295 kb on + strand, within Pf6N2E2_1437 at 1687.295 kb on + strand, within Pf6N2E2_1437 at 1687.344 kb on + strand, within Pf6N2E2_1437
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 1,685,163 - Pf6N2E2_1435 0.42 +0.2 1,685,185 - Pf6N2E2_1435 0.44 +1.0 1,685,284 - Pf6N2E2_1435 0.53 +0.6 1,685,649 + Pf6N2E2_1435 0.87 -1.5 1,685,694 + +0.4 1,685,694 + +0.2 1,685,695 - -0.4 1,685,695 - +0.6 1,685,766 + +0.6 1,685,795 + +0.7 1,685,795 + -1.7 1,685,795 + +0.3 1,685,795 + +0.6 1,685,795 + -0.5 1,685,795 + -1.3 1,685,795 + -0.6 1,685,795 + +1.8 1,685,795 + +1.0 1,685,795 + -0.0 1,685,795 + -0.4 1,685,795 + +0.4 1,685,795 + -1.0 1,685,796 - -0.4 1,685,796 - -0.2 1,685,796 - -1.6 1,685,796 - +1.5 1,685,796 - -0.4 1,685,796 - +0.3 1,685,796 - +0.6 1,685,801 + +0.1 1,685,802 - -1.0 1,685,802 - +2.2 1,685,965 + Pf6N2E2_1436 0.23 -1.7 1,685,965 + Pf6N2E2_1436 0.23 -0.5 1,685,965 + Pf6N2E2_1436 0.23 -1.0 1,685,965 + Pf6N2E2_1436 0.23 -0.7 1,685,965 + Pf6N2E2_1436 0.23 +0.6 1,686,079 - Pf6N2E2_1436 0.43 -2.4 1,686,137 - Pf6N2E2_1436 0.54 -0.6 1,686,137 - Pf6N2E2_1436 0.54 +1.2 1,686,149 - Pf6N2E2_1436 0.56 -0.5 1,686,447 - +0.8 1,686,483 - -0.1 1,687,254 - Pf6N2E2_1437 0.38 +0.6 1,687,256 + Pf6N2E2_1437 0.39 -1.6 1,687,256 + Pf6N2E2_1437 0.39 -0.4 1,687,257 - Pf6N2E2_1437 0.39 -0.4 1,687,270 + Pf6N2E2_1437 0.40 +0.6 1,687,295 + Pf6N2E2_1437 0.42 -2.6 1,687,295 + Pf6N2E2_1437 0.42 -0.6 1,687,344 + Pf6N2E2_1437 0.46 +1.1
Or see this region's nucleotide sequence