Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24610 and BWI76_RS24615 are separated by 280 nucleotides BWI76_RS24615 and BWI76_RS24620 are separated by 236 nucleotides BWI76_RS24620 and BWI76_RS24625 are separated by 113 nucleotides
BWI76_RS24610: BWI76_RS24610 - dihydroxyacetone kinase, at 5,129,618 to 5,131,267
_RS24610
BWI76_RS24615: BWI76_RS24615 - siderophore-interacting protein, at 5,131,548 to 5,132,321
_RS24615
BWI76_RS24620: BWI76_RS24620 - PadR family transcriptional regulator, at 5,132,558 to 5,133,112
_RS24620
BWI76_RS24625: BWI76_RS24625 - hypothetical protein, at 5,133,226 to 5,133,417
_RS24625
Position (kb) 5131
5132
5133 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5130.574 kb on - strand, within BWI76_RS24610 at 5130.611 kb on - strand, within BWI76_RS24610 at 5130.874 kb on + strand, within BWI76_RS24610 at 5131.315 kb on + strand at 5131.714 kb on + strand, within BWI76_RS24615 at 5131.715 kb on + strand, within BWI76_RS24615 at 5131.716 kb on + strand, within BWI76_RS24615 at 5131.722 kb on - strand, within BWI76_RS24615 at 5131.722 kb on - strand at 5131.727 kb on - strand, within BWI76_RS24615 at 5131.806 kb on + strand, within BWI76_RS24615 at 5131.887 kb on - strand, within BWI76_RS24615 at 5132.048 kb on - strand, within BWI76_RS24615 at 5132.058 kb on - strand, within BWI76_RS24615 at 5132.215 kb on - strand, within BWI76_RS24615 at 5132.304 kb on - strand at 5132.304 kb on - strand at 5132.588 kb on - strand at 5132.764 kb on - strand at 5132.940 kb on + strand, within BWI76_RS24620 at 5132.960 kb on - strand, within BWI76_RS24620 at 5132.960 kb on - strand, within BWI76_RS24620 at 5132.960 kb on - strand, within BWI76_RS24620 at 5132.977 kb on + strand, within BWI76_RS24620 at 5133.122 kb on - strand at 5133.188 kb on - strand at 5133.282 kb on + strand, within BWI76_RS24625 at 5133.309 kb on + strand, within BWI76_RS24625 at 5133.317 kb on - strand, within BWI76_RS24625
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 5,130,574 - BWI76_RS24610 0.58 -1.5 5,130,611 - BWI76_RS24610 0.60 +0.4 5,130,874 + BWI76_RS24610 0.76 +1.0 5,131,315 + +0.4 5,131,714 + BWI76_RS24615 0.21 -2.5 5,131,715 + BWI76_RS24615 0.22 -0.8 5,131,716 + BWI76_RS24615 0.22 +1.0 5,131,722 - BWI76_RS24615 0.22 -0.4 5,131,722 - +1.0 5,131,727 - BWI76_RS24615 0.23 -1.8 5,131,806 + BWI76_RS24615 0.33 +0.7 5,131,887 - BWI76_RS24615 0.44 +0.5 5,132,048 - BWI76_RS24615 0.65 -0.1 5,132,058 - BWI76_RS24615 0.66 -0.5 5,132,215 - BWI76_RS24615 0.86 +0.4 5,132,304 - +1.0 5,132,304 - +0.9 5,132,588 - +2.0 5,132,764 - +1.0 5,132,940 + BWI76_RS24620 0.69 -1.3 5,132,960 - BWI76_RS24620 0.72 -1.0 5,132,960 - BWI76_RS24620 0.72 -0.3 5,132,960 - BWI76_RS24620 0.72 -0.1 5,132,977 + BWI76_RS24620 0.75 +0.1 5,133,122 - +1.0 5,133,188 - -0.6 5,133,282 + BWI76_RS24625 0.29 +0.3 5,133,309 + BWI76_RS24625 0.43 +0.7 5,133,317 - BWI76_RS24625 0.47 +0.7
Or see this region's nucleotide sequence