Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19935

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19925 and BWI76_RS19930 are separated by 185 nucleotidesBWI76_RS19930 and BWI76_RS19935 are separated by 43 nucleotidesBWI76_RS19935 and BWI76_RS19940 are separated by 112 nucleotides BWI76_RS19925: BWI76_RS19925 - DNA gyrase subunit A, at 4,171,294 to 4,173,927 _RS19925 BWI76_RS19930: BWI76_RS19930 - bifunctional 3-demethylubiquinone 3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol methylase, at 4,174,113 to 4,174,841 _RS19930 BWI76_RS19935: BWI76_RS19935 - hypothetical protein, at 4,174,885 to 4,175,073 _RS19935 BWI76_RS19940: BWI76_RS19940 - ribonucleoside-diphosphate reductase subunit alpha, at 4,175,186 to 4,177,471 _RS19940 Position (kb) 4174 4175 4176Strain fitness (log2 ratio) -1 0 1at 4173.981 kb on + strandat 4174.048 kb on - strandat 4174.814 kb on + strandat 4174.881 kb on - strandat 4174.881 kb on - strandat 4174.959 kb on + strand, within BWI76_RS19935

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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4,173,981 + -0.0
4,174,048 - -1.5
4,174,814 + +0.3
4,174,881 - +0.0
4,174,881 - -0.7
4,174,959 + BWI76_RS19935 0.39 -1.3

Or see this region's nucleotide sequence