Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19165 and BWI76_RS19170 are separated by 615 nucleotides BWI76_RS19170 and BWI76_RS19175 are separated by 299 nucleotides
BWI76_RS19165: BWI76_RS19165 - chemotaxis protein CheY, at 3,988,214 to 3,988,912
_RS19165
BWI76_RS19170: BWI76_RS19170 - hypothetical protein, at 3,989,528 to 3,990,145
_RS19170
BWI76_RS19175: BWI76_RS19175 - dicarboxylate/amino acid:cation symporter, at 3,990,445 to 3,991,719
_RS19175
Position (kb)
3989
3990
3991 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3988.548 kb on + strand, within BWI76_RS19165 at 3988.651 kb on + strand, within BWI76_RS19165 at 3988.712 kb on - strand, within BWI76_RS19165 at 3988.729 kb on - strand, within BWI76_RS19165 at 3988.745 kb on - strand, within BWI76_RS19165 at 3988.777 kb on + strand, within BWI76_RS19165 at 3988.865 kb on - strand at 3988.906 kb on + strand at 3988.914 kb on - strand at 3988.914 kb on - strand at 3988.949 kb on + strand at 3988.957 kb on - strand at 3989.082 kb on + strand at 3989.090 kb on - strand at 3989.104 kb on + strand at 3989.104 kb on + strand at 3989.108 kb on - strand at 3989.123 kb on + strand at 3989.172 kb on + strand at 3989.172 kb on + strand at 3989.172 kb on + strand at 3989.172 kb on + strand at 3989.180 kb on - strand at 3989.180 kb on - strand at 3989.180 kb on - strand at 3989.180 kb on - strand at 3989.198 kb on + strand at 3989.282 kb on - strand at 3989.294 kb on + strand at 3989.319 kb on - strand at 3989.343 kb on + strand at 3989.344 kb on - strand at 3989.344 kb on - strand at 3989.351 kb on - strand at 3989.351 kb on - strand at 3989.472 kb on - strand at 3989.479 kb on + strand at 3989.481 kb on - strand at 3989.484 kb on + strand at 3989.487 kb on - strand at 3989.491 kb on + strand at 3989.492 kb on - strand at 3989.504 kb on - strand at 3989.504 kb on - strand at 3989.504 kb on - strand at 3989.585 kb on - strand at 3989.594 kb on - strand, within BWI76_RS19170 at 3989.600 kb on - strand, within BWI76_RS19170 at 3989.687 kb on - strand, within BWI76_RS19170 at 3989.695 kb on - strand, within BWI76_RS19170 at 3989.708 kb on - strand, within BWI76_RS19170 at 3989.710 kb on + strand, within BWI76_RS19170 at 3989.710 kb on + strand, within BWI76_RS19170 at 3989.718 kb on - strand, within BWI76_RS19170 at 3989.718 kb on - strand, within BWI76_RS19170 at 3989.718 kb on - strand, within BWI76_RS19170 at 3989.721 kb on + strand, within BWI76_RS19170 at 3989.729 kb on - strand, within BWI76_RS19170 at 3989.729 kb on - strand, within BWI76_RS19170 at 3989.731 kb on - strand, within BWI76_RS19170 at 3989.756 kb on + strand, within BWI76_RS19170 at 3989.764 kb on - strand, within BWI76_RS19170 at 3989.764 kb on - strand, within BWI76_RS19170 at 3989.764 kb on - strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.766 kb on + strand, within BWI76_RS19170 at 3989.769 kb on + strand, within BWI76_RS19170 at 3989.769 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.774 kb on - strand, within BWI76_RS19170 at 3989.814 kb on - strand, within BWI76_RS19170 at 3989.815 kb on - strand, within BWI76_RS19170 at 3989.914 kb on - strand at 3989.914 kb on - strand, within BWI76_RS19170 at 3989.919 kb on - strand, within BWI76_RS19170 at 3989.937 kb on - strand, within BWI76_RS19170 at 3989.937 kb on - strand, within BWI76_RS19170 at 3989.945 kb on - strand, within BWI76_RS19170 at 3989.945 kb on - strand, within BWI76_RS19170 at 3989.945 kb on - strand, within BWI76_RS19170 at 3989.959 kb on + strand, within BWI76_RS19170 at 3990.036 kb on - strand, within BWI76_RS19170 at 3990.036 kb on - strand, within BWI76_RS19170 at 3990.134 kb on - strand at 3990.141 kb on + strand at 3990.148 kb on - strand at 3990.149 kb on - strand at 3990.240 kb on + strand at 3990.254 kb on + strand at 3990.257 kb on - strand at 3990.262 kb on + strand at 3990.268 kb on - strand at 3990.270 kb on - strand at 3990.274 kb on + strand at 3990.327 kb on + strand at 3990.355 kb on + strand at 3990.378 kb on + strand at 3990.382 kb on - strand at 3990.399 kb on + strand at 3990.399 kb on + strand at 3990.407 kb on - strand at 3990.407 kb on - strand at 3990.407 kb on - strand at 3990.407 kb on - strand at 3990.423 kb on + strand at 3990.423 kb on + strand at 3990.430 kb on - strand at 3990.430 kb on - strand at 3990.476 kb on + strand at 3990.536 kb on + strand at 3990.590 kb on - strand at 3990.600 kb on + strand, within BWI76_RS19175 at 3990.638 kb on + strand, within BWI76_RS19175 at 3990.792 kb on - strand, within BWI76_RS19175 at 3990.792 kb on - strand, within BWI76_RS19175 at 3990.797 kb on - strand, within BWI76_RS19175 at 3990.804 kb on + strand, within BWI76_RS19175 at 3990.804 kb on + strand, within BWI76_RS19175 at 3990.804 kb on + strand, within BWI76_RS19175 at 3990.812 kb on - strand, within BWI76_RS19175 at 3990.812 kb on - strand, within BWI76_RS19175 at 3990.874 kb on + strand, within BWI76_RS19175 at 3990.881 kb on - strand, within BWI76_RS19175 at 3990.978 kb on + strand, within BWI76_RS19175 at 3990.986 kb on - strand, within BWI76_RS19175 at 3991.052 kb on + strand, within BWI76_RS19175
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 3,988,548 + BWI76_RS19165 0.48 -0.3 3,988,651 + BWI76_RS19165 0.63 +0.1 3,988,712 - BWI76_RS19165 0.71 -1.2 3,988,729 - BWI76_RS19165 0.74 -0.6 3,988,745 - BWI76_RS19165 0.76 -0.6 3,988,777 + BWI76_RS19165 0.81 -0.0 3,988,865 - +0.5 3,988,906 + -0.8 3,988,914 - +0.3 3,988,914 - +0.7 3,988,949 + +0.4 3,988,957 - +0.3 3,989,082 + +0.6 3,989,090 - -0.8 3,989,104 + -1.1 3,989,104 + +0.0 3,989,108 - -1.7 3,989,123 + +0.1 3,989,172 + +1.6 3,989,172 + -0.8 3,989,172 + -0.5 3,989,172 + +0.3 3,989,180 - +0.1 3,989,180 - -0.1 3,989,180 - -0.4 3,989,180 - +0.0 3,989,198 + -0.5 3,989,282 - +0.3 3,989,294 + -0.1 3,989,319 - -0.3 3,989,343 + -0.5 3,989,344 - -0.9 3,989,344 - -0.8 3,989,351 - +1.3 3,989,351 - -1.1 3,989,472 - +0.8 3,989,479 + +0.4 3,989,481 - +1.0 3,989,484 + -0.2 3,989,487 - -2.0 3,989,491 + +0.1 3,989,492 - -0.5 3,989,504 - -0.3 3,989,504 - +0.3 3,989,504 - +0.3 3,989,585 - +0.2 3,989,594 - BWI76_RS19170 0.11 -0.8 3,989,600 - BWI76_RS19170 0.12 -0.7 3,989,687 - BWI76_RS19170 0.26 -0.4 3,989,695 - BWI76_RS19170 0.27 +1.3 3,989,708 - BWI76_RS19170 0.29 -0.8 3,989,710 + BWI76_RS19170 0.29 -1.4 3,989,710 + BWI76_RS19170 0.29 -2.1 3,989,718 - BWI76_RS19170 0.31 -0.5 3,989,718 - BWI76_RS19170 0.31 -0.2 3,989,718 - BWI76_RS19170 0.31 -0.1 3,989,721 + BWI76_RS19170 0.31 -2.0 3,989,729 - BWI76_RS19170 0.33 -2.8 3,989,729 - BWI76_RS19170 0.33 +1.5 3,989,731 - BWI76_RS19170 0.33 -1.8 3,989,756 + BWI76_RS19170 0.37 -0.9 3,989,764 - BWI76_RS19170 0.38 +0.1 3,989,764 - BWI76_RS19170 0.38 -0.2 3,989,764 - BWI76_RS19170 0.38 -0.6 3,989,766 + BWI76_RS19170 0.39 -0.1 3,989,766 + BWI76_RS19170 0.39 -1.0 3,989,766 + +0.7 3,989,766 + BWI76_RS19170 0.39 -0.6 3,989,766 + BWI76_RS19170 0.39 -0.8 3,989,766 + BWI76_RS19170 0.39 -1.5 3,989,766 + BWI76_RS19170 0.39 +0.0 3,989,766 + BWI76_RS19170 0.39 +0.3 3,989,766 + BWI76_RS19170 0.39 -1.4 3,989,766 + BWI76_RS19170 0.39 -0.5 3,989,766 + BWI76_RS19170 0.39 -4.0 3,989,766 + BWI76_RS19170 0.39 +0.8 3,989,766 + BWI76_RS19170 0.39 -0.2 3,989,766 + BWI76_RS19170 0.39 +0.1 3,989,769 + BWI76_RS19170 0.39 -3.4 3,989,769 - BWI76_RS19170 0.39 -1.3 3,989,774 - BWI76_RS19170 0.40 +0.9 3,989,774 - BWI76_RS19170 0.40 -1.1 3,989,774 - BWI76_RS19170 0.40 +1.0 3,989,774 - BWI76_RS19170 0.40 -1.6 3,989,774 - BWI76_RS19170 0.40 -0.2 3,989,774 - BWI76_RS19170 0.40 +0.5 3,989,774 - BWI76_RS19170 0.40 +0.6 3,989,774 - BWI76_RS19170 0.40 +0.0 3,989,814 - BWI76_RS19170 0.46 +1.3 3,989,815 - BWI76_RS19170 0.46 -2.5 3,989,914 - +0.8 3,989,914 - BWI76_RS19170 0.62 +3.4 3,989,919 - BWI76_RS19170 0.63 +0.1 3,989,937 - BWI76_RS19170 0.66 +0.2 3,989,937 - BWI76_RS19170 0.66 -1.9 3,989,945 - BWI76_RS19170 0.67 +0.1 3,989,945 - BWI76_RS19170 0.67 -1.2 3,989,945 - BWI76_RS19170 0.67 -1.9 3,989,959 + BWI76_RS19170 0.70 +0.0 3,990,036 - BWI76_RS19170 0.82 -0.3 3,990,036 - BWI76_RS19170 0.82 +0.1 3,990,134 - -0.0 3,990,141 + +0.8 3,990,148 - +0.0 3,990,149 - -2.2 3,990,240 + +0.4 3,990,254 + +0.8 3,990,257 - +0.5 3,990,262 + +0.2 3,990,268 - +0.1 3,990,270 - -0.1 3,990,274 + +0.3 3,990,327 + -0.8 3,990,355 + -1.5 3,990,378 + +0.8 3,990,382 - +0.0 3,990,399 + +0.8 3,990,399 + +0.5 3,990,407 - -0.2 3,990,407 - +0.4 3,990,407 - +1.0 3,990,407 - +0.9 3,990,423 + +0.4 3,990,423 + +0.2 3,990,430 - -1.0 3,990,430 - +0.6 3,990,476 + +0.8 3,990,536 + -0.3 3,990,590 - +0.8 3,990,600 + BWI76_RS19175 0.12 -1.1 3,990,638 + BWI76_RS19175 0.15 +0.4 3,990,792 - BWI76_RS19175 0.27 +0.1 3,990,792 - BWI76_RS19175 0.27 -0.3 3,990,797 - BWI76_RS19175 0.28 -0.2 3,990,804 + BWI76_RS19175 0.28 +0.1 3,990,804 + BWI76_RS19175 0.28 +0.4 3,990,804 + BWI76_RS19175 0.28 -2.2 3,990,812 - BWI76_RS19175 0.29 +0.1 3,990,812 - BWI76_RS19175 0.29 +0.1 3,990,874 + BWI76_RS19175 0.34 +0.2 3,990,881 - BWI76_RS19175 0.34 +0.4 3,990,978 + BWI76_RS19175 0.42 -0.4 3,990,986 - BWI76_RS19175 0.42 +0.8 3,991,052 + BWI76_RS19175 0.48 +0.8
Or see this region's nucleotide sequence