Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11140 and BWI76_RS11145 overlap by 2 nucleotides BWI76_RS11145 and BWI76_RS11150 are separated by 5 nucleotides BWI76_RS11150 and BWI76_RS11155 are separated by 12 nucleotides BWI76_RS11155 and BWI76_RS11160 overlap by 20 nucleotides BWI76_RS11160 and BWI76_RS11165 are separated by 19 nucleotides
BWI76_RS11140: BWI76_RS11140 - hypothetical protein, at 2,350,387 to 2,350,635
_RS11140
BWI76_RS11145: BWI76_RS11145 - histidine triad nucleotide-binding protein, at 2,350,634 to 2,350,990
_RS11145
BWI76_RS11150: BWI76_RS11150 - membrane protein, at 2,350,996 to 2,351,370
_RS11150
BWI76_RS11155: BWI76_RS11155 - penicillin-binding protein activator LpoB, at 2,351,383 to 2,352,018
_RS11155
BWI76_RS11160: BWI76_RS11160 - thiamine kinase, at 2,351,999 to 2,352,817
_RS11160
BWI76_RS11165: BWI76_RS11165 - beta-N-acetylhexosaminidase, at 2,352,837 to 2,353,859
_RS11165
Position (kb)
2351
2352
2353 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2351.192 kb on - strand, within BWI76_RS11150 at 2351.319 kb on - strand, within BWI76_RS11150 at 2351.400 kb on - strand at 2351.658 kb on - strand, within BWI76_RS11155 at 2351.881 kb on - strand, within BWI76_RS11155 at 2352.007 kb on - strand at 2352.066 kb on - strand at 2352.951 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 2,351,192 - BWI76_RS11150 0.52 +1.1 2,351,319 - BWI76_RS11150 0.86 -1.3 2,351,400 - -4.9 2,351,658 - BWI76_RS11155 0.43 -3.3 2,351,881 - BWI76_RS11155 0.78 -2.2 2,352,007 - -1.1 2,352,066 - -0.1 2,352,951 - +0.8
Or see this region's nucleotide sequence