Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09505 and BWI76_RS09510 overlap by 1 nucleotides BWI76_RS09510 and BWI76_RS09515 are separated by 6 nucleotides BWI76_RS09515 and BWI76_RS09520 are separated by 19 nucleotides BWI76_RS09520 and BWI76_RS09525 are separated by 227 nucleotides
BWI76_RS09505: BWI76_RS09505 - arginine transporter permease subunit ArtM, at 1,989,836 to 1,990,504
_RS09505
BWI76_RS09510: BWI76_RS09510 - arginine transporter permease subunit ArtQ, at 1,990,504 to 1,991,220
_RS09510
BWI76_RS09515: BWI76_RS09515 - arginine ABC transporter substrate-binding protein, at 1,991,227 to 1,991,958
_RS09515
BWI76_RS09520: BWI76_RS09520 - arginine ABC transporter ATP-binding protein ArtP, at 1,991,978 to 1,992,706
_RS09520
BWI76_RS09525: BWI76_RS09525 - lipoprotein, at 1,992,934 to 1,993,449
_RS09525
Position (kb) 1991
1992 Strain fitness (log2 ratio) -1
0
1
2 at 1990.229 kb on + strand, within BWI76_RS09505 at 1990.365 kb on + strand, within BWI76_RS09505 at 1990.365 kb on + strand, within BWI76_RS09505 at 1991.614 kb on - strand, within BWI76_RS09515 at 1991.655 kb on - strand, within BWI76_RS09515 at 1991.797 kb on - strand, within BWI76_RS09515 at 1992.117 kb on + strand, within BWI76_RS09520 at 1992.383 kb on + strand, within BWI76_RS09520 at 1992.421 kb on - strand, within BWI76_RS09520 at 1992.655 kb on - strand at 1992.704 kb on + strand at 1992.704 kb on + strand at 1992.712 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,990,229 + BWI76_RS09505 0.59 -0.2 1,990,365 + BWI76_RS09505 0.79 -0.7 1,990,365 + BWI76_RS09505 0.79 +0.4 1,991,614 - BWI76_RS09515 0.53 +0.8 1,991,655 - BWI76_RS09515 0.58 +1.4 1,991,797 - BWI76_RS09515 0.78 +2.7 1,992,117 + BWI76_RS09520 0.19 +0.6 1,992,383 + BWI76_RS09520 0.56 +0.0 1,992,421 - BWI76_RS09520 0.61 +0.5 1,992,655 - -0.7 1,992,704 + +0.6 1,992,704 + +0.4 1,992,712 - +0.5
Or see this region's nucleotide sequence