Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08020

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08010 and BWI76_RS08015 are separated by 91 nucleotidesBWI76_RS08015 and BWI76_RS08020 are separated by 106 nucleotidesBWI76_RS08020 and BWI76_RS08025 are separated by 142 nucleotides BWI76_RS08010: BWI76_RS08010 - octanoyltransferase, at 1,698,466 to 1,699,110 _RS08010 BWI76_RS08015: BWI76_RS08015 - hypothetical protein, at 1,699,202 to 1,699,465 _RS08015 BWI76_RS08020: BWI76_RS08020 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,699,572 to 1,700,771 _RS08020 BWI76_RS08025: BWI76_RS08025 - septal ring lytic transglycosylase RlpA, at 1,700,914 to 1,702,014 _RS08025 Position (kb) 1699 1700 1701Strain fitness (log2 ratio) -2 -1 0 1at 1699.151 kb on + strandat 1699.755 kb on - strand, within BWI76_RS08020at 1700.122 kb on - strand, within BWI76_RS08020at 1700.205 kb on + strand, within BWI76_RS08020at 1700.215 kb on + strand, within BWI76_RS08020at 1700.223 kb on - strand, within BWI76_RS08020at 1700.259 kb on - strand, within BWI76_RS08020at 1700.467 kb on + strand, within BWI76_RS08020at 1700.504 kb on + strand, within BWI76_RS08020at 1700.504 kb on + strand, within BWI76_RS08020at 1700.707 kb on + strandat 1700.941 kb on + strandat 1700.949 kb on - strandat 1701.202 kb on + strand, within BWI76_RS08025at 1701.311 kb on - strand, within BWI76_RS08025at 1701.437 kb on + strand, within BWI76_RS08025at 1701.525 kb on + strand, within BWI76_RS08025at 1701.700 kb on - strand, within BWI76_RS08025

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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1,699,151 + -0.3
1,699,755 - BWI76_RS08020 0.15 -1.4
1,700,122 - BWI76_RS08020 0.46 -0.1
1,700,205 + BWI76_RS08020 0.53 -2.3
1,700,215 + BWI76_RS08020 0.54 -1.7
1,700,223 - BWI76_RS08020 0.54 -2.0
1,700,259 - BWI76_RS08020 0.57 -0.8
1,700,467 + BWI76_RS08020 0.75 -0.4
1,700,504 + BWI76_RS08020 0.78 -0.7
1,700,504 + BWI76_RS08020 0.78 -1.9
1,700,707 + -2.5
1,700,941 + +0.3
1,700,949 - -0.7
1,701,202 + BWI76_RS08025 0.26 -1.1
1,701,311 - BWI76_RS08025 0.36 -0.5
1,701,437 + BWI76_RS08025 0.48 -1.3
1,701,525 + BWI76_RS08025 0.55 +0.5
1,701,700 - BWI76_RS08025 0.71 +0.8

Or see this region's nucleotide sequence