Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06725 and BWI76_RS06730 are separated by 269 nucleotides BWI76_RS06730 and BWI76_RS06735 are separated by 111 nucleotides BWI76_RS06735 and BWI76_RS06740 overlap by 1 nucleotides BWI76_RS06740 and BWI76_RS06745 overlap by 4 nucleotides
BWI76_RS06725: BWI76_RS06725 - maltoporin, at 1,411,076 to 1,412,347
_RS06725
BWI76_RS06730: BWI76_RS06730 - lac repressor, at 1,412,617 to 1,413,690
_RS06730
BWI76_RS06735: BWI76_RS06735 - 50S ribosomal protein L31, at 1,413,802 to 1,414,065
_RS06735
BWI76_RS06740: BWI76_RS06740 - 50S ribosomal protein L36, at 1,414,065 to 1,414,205
_RS06740
BWI76_RS06745: BWI76_RS06745 - N-acetyltransferase GCN5, at 1,414,202 to 1,414,900
_RS06745
Position (kb) 1412
1413
1414 Strain fitness (log2 ratio) -1
0
1 at 1411.656 kb on + strand, within BWI76_RS06725 at 1411.732 kb on + strand, within BWI76_RS06725 at 1411.733 kb on + strand, within BWI76_RS06725 at 1411.733 kb on - strand, within BWI76_RS06725 at 1411.748 kb on + strand, within BWI76_RS06725 at 1411.761 kb on - strand, within BWI76_RS06725 at 1411.806 kb on - strand, within BWI76_RS06725 at 1411.999 kb on + strand, within BWI76_RS06725 at 1412.142 kb on - strand, within BWI76_RS06725 at 1412.221 kb on + strand at 1412.229 kb on - strand at 1412.267 kb on - strand at 1412.300 kb on - strand at 1412.300 kb on - strand at 1412.697 kb on - strand at 1413.248 kb on + strand, within BWI76_RS06730 at 1413.536 kb on - strand, within BWI76_RS06730 at 1413.592 kb on + strand at 1413.622 kb on + strand at 1413.657 kb on + strand at 1413.657 kb on + strand at 1413.665 kb on - strand at 1413.907 kb on + strand, within BWI76_RS06735 at 1414.080 kb on + strand, within BWI76_RS06740 at 1414.090 kb on + strand, within BWI76_RS06740 at 1414.169 kb on + strand, within BWI76_RS06740 at 1414.533 kb on + strand, within BWI76_RS06745 at 1414.656 kb on + strand, within BWI76_RS06745
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,411,656 + BWI76_RS06725 0.46 -0.1 1,411,732 + BWI76_RS06725 0.52 +1.6 1,411,733 + BWI76_RS06725 0.52 -0.2 1,411,733 - BWI76_RS06725 0.52 -0.0 1,411,748 + BWI76_RS06725 0.53 -1.4 1,411,761 - BWI76_RS06725 0.54 +0.2 1,411,806 - BWI76_RS06725 0.57 -0.3 1,411,999 + BWI76_RS06725 0.73 -0.1 1,412,142 - BWI76_RS06725 0.84 +0.4 1,412,221 + +1.2 1,412,229 - -0.7 1,412,267 - +0.6 1,412,300 - -1.0 1,412,300 - +0.5 1,412,697 - -0.9 1,413,248 + BWI76_RS06730 0.59 -0.5 1,413,536 - BWI76_RS06730 0.86 -1.0 1,413,592 + -0.8 1,413,622 + -0.9 1,413,657 + +0.4 1,413,657 + -0.2 1,413,665 - +0.2 1,413,907 + BWI76_RS06735 0.40 +0.3 1,414,080 + BWI76_RS06740 0.11 -1.2 1,414,090 + BWI76_RS06740 0.18 +1.0 1,414,169 + BWI76_RS06740 0.74 +0.1 1,414,533 + BWI76_RS06745 0.47 -1.4 1,414,656 + BWI76_RS06745 0.65 +0.3
Or see this region's nucleotide sequence