Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05775 and BWI76_RS05780 overlap by 4 nucleotides BWI76_RS05780 and BWI76_RS05785 overlap by 4 nucleotides BWI76_RS05785 and BWI76_RS05790 overlap by 4 nucleotides BWI76_RS05790 and BWI76_RS05795 are separated by 551 nucleotides
BWI76_RS05775: BWI76_RS05775 - hypothetical protein, at 1,216,908 to 1,217,228
_RS05775
BWI76_RS05780: BWI76_RS05780 - hypothetical protein, at 1,217,225 to 1,217,452
_RS05780
BWI76_RS05785: BWI76_RS05785 - CI repressor, at 1,217,449 to 1,218,000
_RS05785
BWI76_RS05790: BWI76_RS05790 - phage DNA-binding protein, at 1,217,997 to 1,218,263
_RS05790
BWI76_RS05795: BWI76_RS05795 - hypothetical protein, at 1,218,815 to 1,219,552
_RS05795
Position (kb) 1217
1218
1219 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1217.187 kb on + strand, within BWI76_RS05775 at 1217.187 kb on + strand, within BWI76_RS05775 at 1217.190 kb on - strand, within BWI76_RS05775 at 1217.692 kb on - strand, within BWI76_RS05785 at 1217.760 kb on - strand, within BWI76_RS05785 at 1217.812 kb on - strand, within BWI76_RS05785 at 1218.149 kb on + strand, within BWI76_RS05790 at 1218.309 kb on + strand at 1218.354 kb on - strand at 1218.422 kb on - strand at 1218.422 kb on - strand at 1218.756 kb on + strand at 1218.859 kb on + strand at 1218.920 kb on - strand, within BWI76_RS05795 at 1218.934 kb on - strand, within BWI76_RS05795 at 1219.129 kb on + strand, within BWI76_RS05795 at 1219.137 kb on - strand at 1219.192 kb on - strand, within BWI76_RS05795 at 1219.233 kb on - strand, within BWI76_RS05795
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,217,187 + BWI76_RS05775 0.87 -0.1 1,217,187 + BWI76_RS05775 0.87 -1.1 1,217,190 - BWI76_RS05775 0.88 -0.8 1,217,692 - BWI76_RS05785 0.44 -0.0 1,217,760 - BWI76_RS05785 0.56 +0.4 1,217,812 - BWI76_RS05785 0.66 -0.9 1,218,149 + BWI76_RS05790 0.57 -1.0 1,218,309 + +0.7 1,218,354 - -0.1 1,218,422 - -2.9 1,218,422 - +1.3 1,218,756 + +0.2 1,218,859 + +0.0 1,218,920 - BWI76_RS05795 0.14 +0.3 1,218,934 - BWI76_RS05795 0.16 +0.1 1,219,129 + BWI76_RS05795 0.43 -0.1 1,219,137 - -1.1 1,219,192 - BWI76_RS05795 0.51 -0.4 1,219,233 - BWI76_RS05795 0.57 +0.2
Or see this region's nucleotide sequence