Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05240

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05230 and BWI76_RS05235 are separated by 15 nucleotidesBWI76_RS05235 and BWI76_RS05240 overlap by 14 nucleotidesBWI76_RS05240 and BWI76_RS05245 are separated by 12 nucleotidesBWI76_RS05245 and BWI76_RS05250 are separated by 11 nucleotides BWI76_RS05230: BWI76_RS05230 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase, at 1,101,259 to 1,102,458 _RS05230 BWI76_RS05235: BWI76_RS05235 - hypothetical protein, at 1,102,474 to 1,102,714 _RS05235 BWI76_RS05240: BWI76_RS05240 - (2E,6E)-farnesyl- diphosphate-specific ditrans,polycis-undecaprenyl-diphosphate synthase, at 1,102,701 to 1,103,459 _RS05240 BWI76_RS05245: BWI76_RS05245 - phosphatidate cytidylyltransferase, at 1,103,472 to 1,104,329 _RS05245 BWI76_RS05250: BWI76_RS05250 - zinc metallopeptidase RseP, at 1,104,341 to 1,105,693 _RS05250 Position (kb) 1102 1103 1104Strain fitness (log2 ratio) -2 -1 0 1 2at 1102.485 kb on + strandat 1102.500 kb on + strand, within BWI76_RS05235at 1104.336 kb on + strandat 1104.336 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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1,102,485 + +2.3
1,102,500 + BWI76_RS05235 0.11 -2.2
1,104,336 + +0.5
1,104,336 + -0.3

Or see this region's nucleotide sequence