Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02540 and BWI76_RS02545 are separated by 65 nucleotides BWI76_RS02545 and BWI76_RS02550 are separated by 2 nucleotides
BWI76_RS02540: BWI76_RS02540 - GTPase HflX, at 520,341 to 521,621
_RS02540
BWI76_RS02545: BWI76_RS02545 - protease modulator HflK, at 521,687 to 522,943
_RS02545
BWI76_RS02550: BWI76_RS02550 - protease modulator HflC, at 522,946 to 523,950
_RS02550
Position (kb) 521
522
523 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 520.794 kb on - strand, within BWI76_RS02540 at 520.799 kb on - strand, within BWI76_RS02540 at 520.825 kb on - strand, within BWI76_RS02540 at 520.849 kb on + strand, within BWI76_RS02540 at 521.003 kb on + strand, within BWI76_RS02540 at 521.011 kb on - strand, within BWI76_RS02540 at 521.252 kb on + strand, within BWI76_RS02540 at 521.311 kb on + strand, within BWI76_RS02540 at 521.357 kb on + strand, within BWI76_RS02540 at 521.441 kb on + strand, within BWI76_RS02540 at 521.516 kb on - strand at 521.541 kb on + strand at 521.550 kb on - strand at 521.684 kb on + strand at 522.172 kb on - strand, within BWI76_RS02545 at 522.186 kb on - strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.383 kb on - strand, within BWI76_RS02545 at 522.576 kb on - strand, within BWI76_RS02545 at 522.592 kb on + strand, within BWI76_RS02545 at 522.628 kb on + strand, within BWI76_RS02545 at 522.628 kb on + strand, within BWI76_RS02545 at 522.712 kb on + strand, within BWI76_RS02545 at 522.898 kb on - strand at 523.054 kb on + strand, within BWI76_RS02550 at 523.078 kb on + strand, within BWI76_RS02550 at 523.078 kb on + strand, within BWI76_RS02550 at 523.359 kb on - strand, within BWI76_RS02550 at 523.388 kb on + strand, within BWI76_RS02550 at 523.677 kb on - strand, within BWI76_RS02550 at 523.828 kb on + strand, within BWI76_RS02550 at 523.874 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 520,794 - BWI76_RS02540 0.35 +0.6 520,799 - BWI76_RS02540 0.36 -0.8 520,825 - BWI76_RS02540 0.38 -1.4 520,849 + BWI76_RS02540 0.40 +0.7 521,003 + BWI76_RS02540 0.52 +0.1 521,011 - BWI76_RS02540 0.52 +0.1 521,252 + BWI76_RS02540 0.71 +3.1 521,311 + BWI76_RS02540 0.76 -0.3 521,357 + BWI76_RS02540 0.79 +0.3 521,441 + BWI76_RS02540 0.86 +0.5 521,516 - -0.6 521,541 + -1.6 521,550 - -0.3 521,684 + -2.7 522,172 - BWI76_RS02545 0.39 -1.3 522,186 - BWI76_RS02545 0.40 -0.9 522,375 + BWI76_RS02545 0.55 -0.3 522,375 + BWI76_RS02545 0.55 +0.2 522,375 + BWI76_RS02545 0.55 -2.2 522,383 - BWI76_RS02545 0.55 -0.5 522,576 - BWI76_RS02545 0.71 -2.3 522,592 + BWI76_RS02545 0.72 -0.1 522,628 + BWI76_RS02545 0.75 -1.1 522,628 + BWI76_RS02545 0.75 -2.9 522,712 + BWI76_RS02545 0.82 -0.8 522,898 - -0.5 523,054 + BWI76_RS02550 0.11 -0.9 523,078 + BWI76_RS02550 0.13 -0.1 523,078 + BWI76_RS02550 0.13 +1.1 523,359 - BWI76_RS02550 0.41 -0.5 523,388 + BWI76_RS02550 0.44 -1.6 523,677 - BWI76_RS02550 0.73 -1.3 523,828 + BWI76_RS02550 0.88 -1.6 523,874 + -0.9
Or see this region's nucleotide sequence