Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22885 and BWI76_RS22890 are separated by 163 nucleotides BWI76_RS22890 and BWI76_RS22895 are separated by 73 nucleotides
BWI76_RS22885: BWI76_RS22885 - aliphatic sulfonate ABC transporter substrate-binding protein, at 4,775,638 to 4,776,579
_RS22885
BWI76_RS22890: BWI76_RS22890 - 5,10-methylenetetrahydromethanopterin reductase, at 4,776,743 to 4,778,149
_RS22890
BWI76_RS22895: BWI76_RS22895 - FAD-dependent oxidoreductase, at 4,778,223 to 4,779,620
_RS22895
Position (kb) 4776
4777
4778
4779 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4775.824 kb on - strand, within BWI76_RS22885 at 4775.934 kb on - strand, within BWI76_RS22885 at 4775.944 kb on - strand, within BWI76_RS22885 at 4776.081 kb on + strand, within BWI76_RS22885 at 4776.086 kb on + strand, within BWI76_RS22885 at 4776.117 kb on + strand, within BWI76_RS22885 at 4776.171 kb on + strand, within BWI76_RS22885 at 4776.249 kb on - strand, within BWI76_RS22885 at 4776.256 kb on - strand, within BWI76_RS22885 at 4776.266 kb on + strand, within BWI76_RS22885 at 4776.266 kb on - strand, within BWI76_RS22885 at 4776.266 kb on - strand, within BWI76_RS22885 at 4776.267 kb on - strand, within BWI76_RS22885 at 4776.459 kb on + strand, within BWI76_RS22885 at 4776.551 kb on + strand at 4776.573 kb on + strand at 4776.987 kb on + strand, within BWI76_RS22890 at 4776.987 kb on + strand, within BWI76_RS22890 at 4777.188 kb on - strand, within BWI76_RS22890 at 4777.243 kb on - strand, within BWI76_RS22890 at 4777.487 kb on - strand, within BWI76_RS22890 at 4777.569 kb on + strand, within BWI76_RS22890 at 4777.974 kb on + strand, within BWI76_RS22890 at 4778.050 kb on + strand at 4778.081 kb on - strand at 4778.081 kb on - strand at 4778.154 kb on - strand at 4778.154 kb on - strand at 4778.257 kb on - strand at 4778.644 kb on + strand, within BWI76_RS22895 at 4778.658 kb on - strand, within BWI76_RS22895 at 4778.715 kb on - strand, within BWI76_RS22895 at 4778.925 kb on - strand, within BWI76_RS22895 at 4778.925 kb on - strand, within BWI76_RS22895 at 4778.985 kb on - strand, within BWI76_RS22895 at 4779.074 kb on - strand, within BWI76_RS22895 at 4779.079 kb on + strand, within BWI76_RS22895
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,775,824 - BWI76_RS22885 0.20 +0.9 4,775,934 - BWI76_RS22885 0.31 -1.1 4,775,944 - BWI76_RS22885 0.32 +0.7 4,776,081 + BWI76_RS22885 0.47 +0.7 4,776,086 + BWI76_RS22885 0.48 -2.3 4,776,117 + BWI76_RS22885 0.51 -0.0 4,776,171 + BWI76_RS22885 0.57 +0.8 4,776,249 - BWI76_RS22885 0.65 -0.0 4,776,256 - BWI76_RS22885 0.66 -0.7 4,776,266 + BWI76_RS22885 0.67 +0.6 4,776,266 - BWI76_RS22885 0.67 +0.4 4,776,266 - BWI76_RS22885 0.67 -2.2 4,776,267 - BWI76_RS22885 0.67 +0.3 4,776,459 + BWI76_RS22885 0.87 +0.2 4,776,551 + -1.6 4,776,573 + +0.1 4,776,987 + BWI76_RS22890 0.17 +0.3 4,776,987 + BWI76_RS22890 0.17 +0.5 4,777,188 - BWI76_RS22890 0.32 +0.2 4,777,243 - BWI76_RS22890 0.36 -0.1 4,777,487 - BWI76_RS22890 0.53 +0.9 4,777,569 + BWI76_RS22890 0.59 -0.7 4,777,974 + BWI76_RS22890 0.87 +1.8 4,778,050 + +0.1 4,778,081 - -0.2 4,778,081 - -0.2 4,778,154 - +0.2 4,778,154 - -0.5 4,778,257 - -0.1 4,778,644 + BWI76_RS22895 0.30 -0.4 4,778,658 - BWI76_RS22895 0.31 +0.3 4,778,715 - BWI76_RS22895 0.35 -0.5 4,778,925 - BWI76_RS22895 0.50 +1.9 4,778,925 - BWI76_RS22895 0.50 +0.9 4,778,985 - BWI76_RS22895 0.55 +0.3 4,779,074 - BWI76_RS22895 0.61 +0.2 4,779,079 + BWI76_RS22895 0.61 -0.2
Or see this region's nucleotide sequence