Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21055 and BWI76_RS21060 are separated by 58 nucleotides BWI76_RS21060 and BWI76_RS21065 are separated by 158 nucleotides BWI76_RS21065 and BWI76_RS21070 are separated by 114 nucleotides
BWI76_RS21055: BWI76_RS21055 - Tol-Pal system TolA, at 4,405,459 to 4,405,851
_RS21055
BWI76_RS21060: BWI76_RS21060 - transporter TonB, at 4,405,910 to 4,406,287
_RS21060
BWI76_RS21065: BWI76_RS21065 - ribosome biogenesis GTPase Der, at 4,406,446 to 4,407,924
_RS21065
BWI76_RS21070: BWI76_RS21070 - outer membrane protein assembly factor BamB, at 4,408,039 to 4,409,217
_RS21070
Position (kb) 4406
4407
4408 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4405.483 kb on + strand at 4405.483 kb on + strand at 4405.486 kb on - strand at 4405.507 kb on - strand, within BWI76_RS21055 at 4405.509 kb on + strand, within BWI76_RS21055 at 4405.524 kb on + strand, within BWI76_RS21055 at 4405.552 kb on + strand, within BWI76_RS21055 at 4405.555 kb on - strand, within BWI76_RS21055 at 4405.590 kb on + strand, within BWI76_RS21055 at 4405.595 kb on + strand, within BWI76_RS21055 at 4405.600 kb on + strand, within BWI76_RS21055 at 4405.604 kb on + strand, within BWI76_RS21055 at 4405.608 kb on - strand, within BWI76_RS21055 at 4405.612 kb on - strand, within BWI76_RS21055 at 4405.672 kb on - strand, within BWI76_RS21055 at 4405.700 kb on + strand, within BWI76_RS21055 at 4405.708 kb on - strand, within BWI76_RS21055 at 4405.753 kb on + strand, within BWI76_RS21055 at 4405.761 kb on - strand, within BWI76_RS21055 at 4405.769 kb on - strand, within BWI76_RS21055 at 4405.787 kb on + strand, within BWI76_RS21055 at 4405.795 kb on - strand, within BWI76_RS21055 at 4405.797 kb on + strand, within BWI76_RS21055 at 4405.797 kb on + strand, within BWI76_RS21055 at 4405.797 kb on + strand, within BWI76_RS21055 at 4405.880 kb on + strand at 4405.886 kb on + strand at 4405.900 kb on + strand at 4405.908 kb on - strand at 4405.931 kb on + strand at 4405.931 kb on + strand at 4405.931 kb on + strand at 4405.934 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.939 kb on - strand at 4405.959 kb on - strand, within BWI76_RS21060 at 4405.959 kb on - strand, within BWI76_RS21060 at 4405.966 kb on + strand, within BWI76_RS21060 at 4405.966 kb on + strand, within BWI76_RS21060 at 4405.966 kb on + strand, within BWI76_RS21060 at 4405.966 kb on + strand, within BWI76_RS21060 at 4405.971 kb on + strand, within BWI76_RS21060 at 4405.974 kb on - strand, within BWI76_RS21060 at 4405.974 kb on - strand, within BWI76_RS21060 at 4405.981 kb on + strand, within BWI76_RS21060 at 4405.994 kb on - strand, within BWI76_RS21060 at 4406.023 kb on + strand, within BWI76_RS21060 at 4406.023 kb on - strand, within BWI76_RS21060 at 4406.030 kb on - strand, within BWI76_RS21060 at 4406.066 kb on - strand, within BWI76_RS21060 at 4406.080 kb on + strand, within BWI76_RS21060 at 4406.089 kb on + strand, within BWI76_RS21060 at 4406.126 kb on + strand, within BWI76_RS21060 at 4406.148 kb on + strand, within BWI76_RS21060 at 4406.148 kb on + strand, within BWI76_RS21060 at 4406.153 kb on + strand, within BWI76_RS21060 at 4406.153 kb on + strand, within BWI76_RS21060 at 4406.153 kb on + strand, within BWI76_RS21060 at 4406.161 kb on - strand, within BWI76_RS21060 at 4406.203 kb on - strand, within BWI76_RS21060 at 4406.207 kb on - strand, within BWI76_RS21060 at 4406.207 kb on - strand at 4406.207 kb on - strand, within BWI76_RS21060 at 4406.207 kb on - strand, within BWI76_RS21060 at 4406.210 kb on + strand, within BWI76_RS21060 at 4406.220 kb on - strand, within BWI76_RS21060 at 4406.220 kb on - strand, within BWI76_RS21060 at 4406.235 kb on + strand, within BWI76_RS21060 at 4406.235 kb on + strand, within BWI76_RS21060 at 4406.240 kb on + strand, within BWI76_RS21060 at 4406.240 kb on + strand, within BWI76_RS21060 at 4406.248 kb on - strand, within BWI76_RS21060 at 4406.256 kb on - strand at 4406.256 kb on - strand at 4406.294 kb on - strand at 4406.294 kb on - strand at 4408.334 kb on - strand, within BWI76_RS21070 at 4408.461 kb on - strand, within BWI76_RS21070
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,405,483 + +0.3 4,405,483 + +0.5 4,405,486 - -1.0 4,405,507 - BWI76_RS21055 0.12 +0.3 4,405,509 + BWI76_RS21055 0.13 +0.3 4,405,524 + BWI76_RS21055 0.17 +0.9 4,405,552 + BWI76_RS21055 0.24 -1.1 4,405,555 - BWI76_RS21055 0.24 +1.0 4,405,590 + BWI76_RS21055 0.33 -0.5 4,405,595 + BWI76_RS21055 0.35 -0.9 4,405,600 + BWI76_RS21055 0.36 +0.7 4,405,604 + BWI76_RS21055 0.37 +0.0 4,405,608 - BWI76_RS21055 0.38 -1.9 4,405,612 - BWI76_RS21055 0.39 -0.1 4,405,672 - BWI76_RS21055 0.54 +0.2 4,405,700 + BWI76_RS21055 0.61 -0.2 4,405,708 - BWI76_RS21055 0.63 +0.3 4,405,753 + BWI76_RS21055 0.75 +1.0 4,405,761 - BWI76_RS21055 0.77 +0.5 4,405,769 - BWI76_RS21055 0.79 -0.3 4,405,787 + BWI76_RS21055 0.83 -0.2 4,405,795 - BWI76_RS21055 0.85 +0.7 4,405,797 + BWI76_RS21055 0.86 -0.1 4,405,797 + BWI76_RS21055 0.86 +0.8 4,405,797 + BWI76_RS21055 0.86 -0.2 4,405,880 + +0.4 4,405,886 + -2.3 4,405,900 + -0.1 4,405,908 - -0.1 4,405,931 + +0.6 4,405,931 + +0.0 4,405,931 + +0.6 4,405,934 - +0.2 4,405,939 - +0.5 4,405,939 - -0.3 4,405,939 - +0.2 4,405,939 - +0.7 4,405,939 - -0.7 4,405,939 - -0.9 4,405,939 - -0.3 4,405,939 - +0.4 4,405,939 - +0.4 4,405,939 - -1.3 4,405,959 - BWI76_RS21060 0.13 -0.1 4,405,959 - BWI76_RS21060 0.13 -2.9 4,405,966 + BWI76_RS21060 0.15 -0.0 4,405,966 + BWI76_RS21060 0.15 +0.2 4,405,966 + BWI76_RS21060 0.15 -2.3 4,405,966 + BWI76_RS21060 0.15 +1.0 4,405,971 + BWI76_RS21060 0.16 -1.9 4,405,974 - BWI76_RS21060 0.17 +1.4 4,405,974 - BWI76_RS21060 0.17 -0.4 4,405,981 + BWI76_RS21060 0.19 +1.1 4,405,994 - BWI76_RS21060 0.22 +0.9 4,406,023 + BWI76_RS21060 0.30 -0.7 4,406,023 - BWI76_RS21060 0.30 +0.5 4,406,030 - BWI76_RS21060 0.32 -0.2 4,406,066 - BWI76_RS21060 0.41 -1.9 4,406,080 + BWI76_RS21060 0.45 -1.2 4,406,089 + BWI76_RS21060 0.47 -1.9 4,406,126 + BWI76_RS21060 0.57 +0.0 4,406,148 + BWI76_RS21060 0.63 +0.3 4,406,148 + BWI76_RS21060 0.63 -0.4 4,406,153 + BWI76_RS21060 0.64 +1.5 4,406,153 + BWI76_RS21060 0.64 -0.9 4,406,153 + BWI76_RS21060 0.64 -0.7 4,406,161 - BWI76_RS21060 0.66 -0.1 4,406,203 - BWI76_RS21060 0.78 -1.2 4,406,207 - BWI76_RS21060 0.79 -2.9 4,406,207 - +0.9 4,406,207 - BWI76_RS21060 0.79 +1.0 4,406,207 - BWI76_RS21060 0.79 -0.7 4,406,210 + BWI76_RS21060 0.79 +0.0 4,406,220 - BWI76_RS21060 0.82 -0.7 4,406,220 - BWI76_RS21060 0.82 -1.0 4,406,235 + BWI76_RS21060 0.86 -1.0 4,406,235 + BWI76_RS21060 0.86 +0.7 4,406,240 + BWI76_RS21060 0.87 +1.1 4,406,240 + BWI76_RS21060 0.87 -0.5 4,406,248 - BWI76_RS21060 0.89 -1.1 4,406,256 - -1.3 4,406,256 - -0.4 4,406,294 - -1.2 4,406,294 - -0.1 4,408,334 - BWI76_RS21070 0.25 -2.1 4,408,461 - BWI76_RS21070 0.36 +0.9
Or see this region's nucleotide sequence