Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16110 and BWI76_RS16115 are separated by 52 nucleotides BWI76_RS16115 and BWI76_RS16120 are separated by 83 nucleotides BWI76_RS16120 and BWI76_RS16125 overlap by 11 nucleotides
BWI76_RS16110: BWI76_RS16110 - L-tartrate/succinate antiporter, at 3,341,961 to 3,343,466
_RS16110
BWI76_RS16115: BWI76_RS16115 - fumarate hydratase, at 3,343,519 to 3,345,174
_RS16115
BWI76_RS16120: BWI76_RS16120 - 4-carboxymuconolactone decarboxylase, at 3,345,258 to 3,345,641
_RS16120
BWI76_RS16125: BWI76_RS16125 - 3-oxoadipate enol-lactonase, at 3,345,631 to 3,346,398
_RS16125
Position (kb) 3343
3344
3345
3346 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3342.538 kb on + strand, within BWI76_RS16110 at 3342.582 kb on - strand, within BWI76_RS16110 at 3342.592 kb on - strand, within BWI76_RS16110 at 3342.643 kb on + strand, within BWI76_RS16110 at 3343.663 kb on - strand at 3343.663 kb on - strand at 3343.929 kb on + strand, within BWI76_RS16115 at 3344.316 kb on - strand, within BWI76_RS16115 at 3344.316 kb on - strand, within BWI76_RS16115 at 3344.603 kb on + strand, within BWI76_RS16115 at 3344.637 kb on + strand, within BWI76_RS16115 at 3344.689 kb on - strand, within BWI76_RS16115 at 3344.746 kb on - strand, within BWI76_RS16115 at 3345.032 kb on + strand at 3345.032 kb on + strand at 3345.228 kb on + strand at 3345.392 kb on - strand, within BWI76_RS16120 at 3345.440 kb on + strand, within BWI76_RS16120 at 3345.585 kb on + strand, within BWI76_RS16120 at 3345.593 kb on - strand, within BWI76_RS16120 at 3345.654 kb on + strand at 3345.903 kb on + strand, within BWI76_RS16125
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,342,538 + BWI76_RS16110 0.38 -0.6 3,342,582 - BWI76_RS16110 0.41 -0.0 3,342,592 - BWI76_RS16110 0.42 +0.7 3,342,643 + BWI76_RS16110 0.45 +0.1 3,343,663 - +1.5 3,343,663 - +0.3 3,343,929 + BWI76_RS16115 0.25 +0.9 3,344,316 - BWI76_RS16115 0.48 +0.9 3,344,316 - BWI76_RS16115 0.48 +1.9 3,344,603 + BWI76_RS16115 0.65 +0.1 3,344,637 + BWI76_RS16115 0.68 +1.1 3,344,689 - BWI76_RS16115 0.71 +0.9 3,344,746 - BWI76_RS16115 0.74 +1.3 3,345,032 + -0.6 3,345,032 + -0.4 3,345,228 + -0.4 3,345,392 - BWI76_RS16120 0.35 -1.1 3,345,440 + BWI76_RS16120 0.47 +0.6 3,345,585 + BWI76_RS16120 0.85 +0.9 3,345,593 - BWI76_RS16120 0.87 +0.3 3,345,654 + +0.4 3,345,903 + BWI76_RS16125 0.35 -2.1
Or see this region's nucleotide sequence