Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15695

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15675 and BWI76_RS15680 are separated by 111 nucleotidesBWI76_RS15680 and BWI76_RS15685 are separated by 85 nucleotidesBWI76_RS15685 and BWI76_RS15690 overlap by 50 nucleotidesBWI76_RS15690 and BWI76_RS15695 overlap by 4 nucleotidesBWI76_RS15695 and BWI76_RS15700 overlap by 4 nucleotidesBWI76_RS15700 and BWI76_RS15705 overlap by 4 nucleotides BWI76_RS15675: BWI76_RS15675 - hypothetical protein, at 3,275,020 to 3,275,265 _RS15675 BWI76_RS15680: BWI76_RS15680 - hypothetical protein, at 3,275,377 to 3,275,562 _RS15680 BWI76_RS15685: BWI76_RS15685 - hypothetical protein, at 3,275,648 to 3,275,839 _RS15685 BWI76_RS15690: BWI76_RS15690 - hypothetical protein, at 3,275,790 to 3,276,065 _RS15690 BWI76_RS15695: BWI76_RS15695 - hypothetical protein, at 3,276,062 to 3,276,406 _RS15695 BWI76_RS15700: BWI76_RS15700 - hypothetical protein, at 3,276,403 to 3,276,945 _RS15700 BWI76_RS15705: BWI76_RS15705 - putative holin protein, at 3,276,942 to 3,277,253 _RS15705 Position (kb) 3276 3277Strain fitness (log2 ratio) -2 -1 0 1 2at 3275.165 kb on + strand, within BWI76_RS15675at 3275.186 kb on + strand, within BWI76_RS15675at 3275.194 kb on - strand, within BWI76_RS15675at 3275.194 kb on - strand, within BWI76_RS15675at 3275.194 kb on - strand, within BWI76_RS15675at 3275.221 kb on - strand, within BWI76_RS15675at 3275.221 kb on - strand, within BWI76_RS15675at 3275.278 kb on + strandat 3275.322 kb on - strandat 3275.491 kb on + strand, within BWI76_RS15680at 3275.514 kb on - strand, within BWI76_RS15680at 3275.533 kb on + strand, within BWI76_RS15680at 3275.611 kb on + strandat 3275.619 kb on - strandat 3275.676 kb on - strand, within BWI76_RS15685at 3275.808 kb on + strandat 3275.959 kb on + strand, within BWI76_RS15690at 3276.178 kb on + strand, within BWI76_RS15695at 3276.218 kb on - strand, within BWI76_RS15695at 3276.253 kb on - strandat 3276.255 kb on - strand, within BWI76_RS15695at 3276.538 kb on - strand, within BWI76_RS15700at 3276.543 kb on - strand, within BWI76_RS15700at 3276.606 kb on - strand, within BWI76_RS15700at 3276.636 kb on + strand, within BWI76_RS15700at 3276.659 kb on + strand, within BWI76_RS15700at 3276.826 kb on - strand, within BWI76_RS15700at 3276.971 kb on + strandat 3276.989 kb on - strand, within BWI76_RS15705at 3276.999 kb on - strand, within BWI76_RS15705at 3277.031 kb on - strand, within BWI76_RS15705at 3277.062 kb on + strand, within BWI76_RS15705at 3277.212 kb on - strand, within BWI76_RS15705at 3277.243 kb on + strandat 3277.251 kb on - strandat 3277.300 kb on + strandat 3277.307 kb on - strandat 3277.308 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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3,275,165 + BWI76_RS15675 0.59 -0.3
3,275,186 + BWI76_RS15675 0.67 -0.1
3,275,194 - BWI76_RS15675 0.71 +1.9
3,275,194 - BWI76_RS15675 0.71 -1.1
3,275,194 - BWI76_RS15675 0.71 +0.1
3,275,221 - BWI76_RS15675 0.82 -1.3
3,275,221 - BWI76_RS15675 0.82 -0.3
3,275,278 + -1.3
3,275,322 - -0.7
3,275,491 + BWI76_RS15680 0.61 -0.8
3,275,514 - BWI76_RS15680 0.74 -0.7
3,275,533 + BWI76_RS15680 0.84 -1.0
3,275,611 + +1.2
3,275,619 - -1.3
3,275,676 - BWI76_RS15685 0.15 -0.4
3,275,808 + -0.5
3,275,959 + BWI76_RS15690 0.61 -1.1
3,276,178 + BWI76_RS15695 0.34 +0.2
3,276,218 - BWI76_RS15695 0.45 -0.6
3,276,253 - -0.7
3,276,255 - BWI76_RS15695 0.56 -1.4
3,276,538 - BWI76_RS15700 0.25 -0.1
3,276,543 - BWI76_RS15700 0.26 -0.8
3,276,606 - BWI76_RS15700 0.37 -2.4
3,276,636 + BWI76_RS15700 0.43 -0.7
3,276,659 + BWI76_RS15700 0.47 -0.9
3,276,826 - BWI76_RS15700 0.78 -0.3
3,276,971 + -0.5
3,276,989 - BWI76_RS15705 0.15 -1.6
3,276,999 - BWI76_RS15705 0.18 -0.7
3,277,031 - BWI76_RS15705 0.29 -0.7
3,277,062 + BWI76_RS15705 0.38 -1.2
3,277,212 - BWI76_RS15705 0.87 -0.5
3,277,243 + -0.4
3,277,251 - -0.6
3,277,300 + -0.7
3,277,307 - -1.6
3,277,308 - -0.8

Or see this region's nucleotide sequence