Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04100 and BWI76_RS04105 are separated by 217 nucleotides BWI76_RS04105 and BWI76_RS04110 are separated by 123 nucleotides BWI76_RS04110 and BWI76_RS04115 are separated by 120 nucleotides
BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646
_RS04100
BWI76_RS04105: BWI76_RS04105 - molecular chaperone OsmY, at 844,864 to 845,475
_RS04105
BWI76_RS04110: BWI76_RS04110 - DUF1328 domain-containing protein, at 845,599 to 845,760
_RS04110
BWI76_RS04115: BWI76_RS04115 - patatin family protein, at 845,881 to 846,954
_RS04115
Position (kb) 845
846 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 844.899 kb on - strand at 844.923 kb on - strand at 844.951 kb on + strand, within BWI76_RS04105 at 844.951 kb on + strand, within BWI76_RS04105 at 844.951 kb on + strand, within BWI76_RS04105 at 844.959 kb on - strand, within BWI76_RS04105 at 845.063 kb on - strand, within BWI76_RS04105 at 845.063 kb on - strand, within BWI76_RS04105 at 845.097 kb on + strand, within BWI76_RS04105 at 845.133 kb on - strand, within BWI76_RS04105 at 845.141 kb on + strand, within BWI76_RS04105 at 845.259 kb on + strand, within BWI76_RS04105 at 845.368 kb on - strand, within BWI76_RS04105 at 845.385 kb on - strand, within BWI76_RS04105 at 845.409 kb on + strand, within BWI76_RS04105 at 845.436 kb on - strand at 845.527 kb on + strand at 845.527 kb on + strand at 845.527 kb on + strand at 845.527 kb on - strand at 845.535 kb on - strand at 845.535 kb on - strand at 845.540 kb on - strand at 845.582 kb on + strand at 845.589 kb on + strand at 845.590 kb on - strand at 845.647 kb on + strand, within BWI76_RS04110 at 845.655 kb on - strand, within BWI76_RS04110 at 845.657 kb on + strand, within BWI76_RS04110 at 845.667 kb on + strand, within BWI76_RS04110 at 845.687 kb on - strand, within BWI76_RS04110 at 845.711 kb on + strand, within BWI76_RS04110 at 845.763 kb on - strand at 845.907 kb on + strand at 845.912 kb on + strand at 845.951 kb on + strand at 845.959 kb on - strand at 845.959 kb on - strand at 845.964 kb on - strand at 845.971 kb on + strand at 845.980 kb on - strand at 846.420 kb on - strand, within BWI76_RS04115 at 846.514 kb on + strand, within BWI76_RS04115 at 846.519 kb on + strand, within BWI76_RS04115 at 846.521 kb on + strand, within BWI76_RS04115 at 846.527 kb on - strand, within BWI76_RS04115 at 846.633 kb on - strand, within BWI76_RS04115 at 846.711 kb on - strand, within BWI76_RS04115 at 846.711 kb on - strand, within BWI76_RS04115 at 846.746 kb on - strand, within BWI76_RS04115
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 844,899 - -1.4 844,923 - -0.5 844,951 + BWI76_RS04105 0.14 +0.1 844,951 + BWI76_RS04105 0.14 +0.9 844,951 + BWI76_RS04105 0.14 +0.2 844,959 - BWI76_RS04105 0.16 +0.1 845,063 - BWI76_RS04105 0.33 -0.9 845,063 - BWI76_RS04105 0.33 +0.3 845,097 + BWI76_RS04105 0.38 +0.5 845,133 - BWI76_RS04105 0.44 -0.1 845,141 + BWI76_RS04105 0.45 +0.3 845,259 + BWI76_RS04105 0.65 +0.3 845,368 - BWI76_RS04105 0.82 -1.1 845,385 - BWI76_RS04105 0.85 +0.2 845,409 + BWI76_RS04105 0.89 -0.8 845,436 - +0.2 845,527 + -0.2 845,527 + -1.2 845,527 + -0.3 845,527 - -0.7 845,535 - +0.6 845,535 - +0.9 845,540 - +0.4 845,582 + -0.1 845,589 + +0.9 845,590 - -0.4 845,647 + BWI76_RS04110 0.30 +0.4 845,655 - BWI76_RS04110 0.35 +0.2 845,657 + BWI76_RS04110 0.36 +0.9 845,667 + BWI76_RS04110 0.42 +1.5 845,687 - BWI76_RS04110 0.54 -1.1 845,711 + BWI76_RS04110 0.69 +0.3 845,763 - +0.8 845,907 + +0.2 845,912 + -0.4 845,951 + -3.2 845,959 - -0.1 845,959 - +0.2 845,964 - +0.1 845,971 + -0.8 845,980 - +1.9 846,420 - BWI76_RS04115 0.50 -0.4 846,514 + BWI76_RS04115 0.59 +0.3 846,519 + BWI76_RS04115 0.59 -2.8 846,521 + BWI76_RS04115 0.60 -0.1 846,527 - BWI76_RS04115 0.60 -1.6 846,633 - BWI76_RS04115 0.70 -0.7 846,711 - BWI76_RS04115 0.77 +0.3 846,711 - BWI76_RS04115 0.77 -0.7 846,746 - BWI76_RS04115 0.81 -0.1
Or see this region's nucleotide sequence