Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03830 and BWI76_RS03835 are separated by 378 nucleotides BWI76_RS03835 and BWI76_RS03840 are separated by 9 nucleotides
BWI76_RS03830: BWI76_RS03830 - 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component, at 791,891 to 793,453
_RS03830
BWI76_RS03835: BWI76_RS03835 - 4-hydroxyphenylacetate catabolism regulatory protein HpaA, at 793,832 to 794,722
_RS03835
BWI76_RS03840: BWI76_RS03840 - 4-hydroxyphenylacetate permease, at 794,732 to 796,108
_RS03840
Position (kb)
793
794
795 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 792.851 kb on + strand, within BWI76_RS03830 at 792.954 kb on + strand at 793.098 kb on + strand, within BWI76_RS03830 at 793.218 kb on - strand, within BWI76_RS03830 at 793.264 kb on + strand, within BWI76_RS03830 at 793.269 kb on + strand, within BWI76_RS03830 at 793.272 kb on - strand, within BWI76_RS03830 at 793.350 kb on - strand at 793.381 kb on + strand at 793.389 kb on - strand at 793.400 kb on + strand at 793.421 kb on + strand at 793.571 kb on - strand at 793.590 kb on - strand at 793.628 kb on + strand at 794.012 kb on + strand, within BWI76_RS03835 at 794.108 kb on - strand, within BWI76_RS03835 at 794.159 kb on + strand, within BWI76_RS03835 at 794.411 kb on + strand, within BWI76_RS03835 at 794.537 kb on - strand, within BWI76_RS03835 at 794.824 kb on - strand at 794.873 kb on + strand, within BWI76_RS03840 at 795.009 kb on + strand, within BWI76_RS03840 at 795.017 kb on - strand, within BWI76_RS03840 at 795.104 kb on + strand, within BWI76_RS03840 at 795.315 kb on + strand, within BWI76_RS03840 at 795.351 kb on - strand, within BWI76_RS03840 at 795.656 kb on - strand, within BWI76_RS03840 at 795.700 kb on + strand, within BWI76_RS03840
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 792,851 + BWI76_RS03830 0.61 +0.4 792,954 + +1.0 793,098 + BWI76_RS03830 0.77 -0.3 793,218 - BWI76_RS03830 0.85 +2.0 793,264 + BWI76_RS03830 0.88 -0.9 793,269 + BWI76_RS03830 0.88 +0.5 793,272 - BWI76_RS03830 0.88 +0.9 793,350 - -0.5 793,381 + +0.3 793,389 - +0.2 793,400 + +0.6 793,421 + +0.5 793,571 - -0.3 793,590 - -0.1 793,628 + -0.6 794,012 + BWI76_RS03835 0.20 +0.2 794,108 - BWI76_RS03835 0.31 +0.8 794,159 + BWI76_RS03835 0.37 +1.5 794,411 + BWI76_RS03835 0.65 +1.0 794,537 - BWI76_RS03835 0.79 +0.1 794,824 - -0.4 794,873 + BWI76_RS03840 0.10 +3.8 795,009 + BWI76_RS03840 0.20 -2.7 795,017 - BWI76_RS03840 0.21 -0.0 795,104 + BWI76_RS03840 0.27 -0.1 795,315 + BWI76_RS03840 0.42 -2.1 795,351 - BWI76_RS03840 0.45 +0.3 795,656 - BWI76_RS03840 0.67 +0.1 795,700 + BWI76_RS03840 0.70 -0.8
Or see this region's nucleotide sequence