Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03600 and BWI76_RS03605 are separated by 17 nucleotides BWI76_RS03605 and BWI76_RS03610 are separated by 406 nucleotides BWI76_RS03610 and BWI76_RS03615 overlap by 4 nucleotides BWI76_RS03615 and BWI76_RS03620 are separated by 17 nucleotides
BWI76_RS03600: BWI76_RS03600 - BMC domain-containing protein, at 746,257 to 746,541
_RS03600
BWI76_RS03605: BWI76_RS03605 - BMC domain-containing protein, at 746,559 to 746,837
_RS03605
BWI76_RS03610: BWI76_RS03610 - hypothetical protein, at 747,244 to 747,762
_RS03610
BWI76_RS03615: BWI76_RS03615 - putative transcriptional regulatory protein, at 747,759 to 748,559
_RS03615
BWI76_RS03620: BWI76_RS03620 - TetR family transcriptional regulator, at 748,577 to 749,161
_RS03620
Position (kb)
747
748 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 746.505 kb on - strand, within BWI76_RS03600 at 746.523 kb on + strand at 746.557 kb on - strand at 746.557 kb on - strand at 746.695 kb on + strand, within BWI76_RS03605 at 746.725 kb on - strand, within BWI76_RS03605 at 746.769 kb on - strand, within BWI76_RS03605 at 746.819 kb on + strand at 746.819 kb on + strand at 746.827 kb on - strand at 746.827 kb on - strand at 747.301 kb on + strand, within BWI76_RS03610 at 747.338 kb on + strand, within BWI76_RS03610 at 747.652 kb on + strand, within BWI76_RS03610 at 747.722 kb on + strand at 747.809 kb on + strand at 747.856 kb on - strand, within BWI76_RS03615 at 748.031 kb on + strand, within BWI76_RS03615 at 748.040 kb on + strand, within BWI76_RS03615 at 748.255 kb on + strand, within BWI76_RS03615 at 748.265 kb on + strand, within BWI76_RS03615 at 748.265 kb on + strand, within BWI76_RS03615 at 748.265 kb on + strand, within BWI76_RS03615 at 748.369 kb on + strand, within BWI76_RS03615 at 748.574 kb on + strand at 748.728 kb on + strand, within BWI76_RS03620
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 746,505 - BWI76_RS03600 0.87 -2.6 746,523 + -1.9 746,557 - -0.4 746,557 - -0.0 746,695 + BWI76_RS03605 0.49 +1.0 746,725 - BWI76_RS03605 0.59 +0.3 746,769 - BWI76_RS03605 0.75 +0.5 746,819 + +0.8 746,819 + +1.6 746,827 - +0.6 746,827 - +1.1 747,301 + BWI76_RS03610 0.11 +0.3 747,338 + BWI76_RS03610 0.18 +0.7 747,652 + BWI76_RS03610 0.79 +1.0 747,722 + +2.4 747,809 + +1.0 747,856 - BWI76_RS03615 0.12 -1.4 748,031 + BWI76_RS03615 0.34 +0.5 748,040 + BWI76_RS03615 0.35 +0.5 748,255 + BWI76_RS03615 0.62 -0.6 748,265 + BWI76_RS03615 0.63 +1.0 748,265 + BWI76_RS03615 0.63 -0.6 748,265 + BWI76_RS03615 0.63 +0.4 748,369 + BWI76_RS03615 0.76 +0.8 748,574 + +0.4 748,728 + BWI76_RS03620 0.26 -0.6
Or see this region's nucleotide sequence