Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02185 and BWI76_RS02190 are separated by 14 nucleotides BWI76_RS02190 and BWI76_RS02195 overlap by 1 nucleotides BWI76_RS02195 and BWI76_RS02200 overlap by 4 nucleotides BWI76_RS02200 and BWI76_RS02205 are separated by 196 nucleotides
BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167
_RS02185
BWI76_RS02190: BWI76_RS02190 - ribose 1,5-bisphosphokinase, at 454,182 to 454,745
_RS02190
BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881
_RS02195
BWI76_RS02200: BWI76_RS02200 - phosphonate C-P lyase system protein PhnL, at 455,878 to 456,564
_RS02200
BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519
_RS02205
Position (kb) 454
455
456 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 453.803 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.830 kb on + strand, within BWI76_RS02185 at 453.848 kb on - strand, within BWI76_RS02185 at 453.943 kb on + strand, within BWI76_RS02185 at 454.169 kb on - strand at 454.274 kb on + strand, within BWI76_RS02190 at 454.343 kb on + strand, within BWI76_RS02190 at 454.559 kb on + strand, within BWI76_RS02190 at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.763 kb on - strand at 454.797 kb on + strand at 454.920 kb on - strand at 454.931 kb on + strand, within BWI76_RS02195 at 455.083 kb on - strand, within BWI76_RS02195 at 455.091 kb on - strand, within BWI76_RS02195 at 455.298 kb on - strand, within BWI76_RS02195 at 455.367 kb on + strand, within BWI76_RS02195 at 455.479 kb on + strand, within BWI76_RS02195 at 455.618 kb on - strand, within BWI76_RS02195 at 455.656 kb on - strand, within BWI76_RS02195 at 455.667 kb on + strand, within BWI76_RS02195 at 455.680 kb on - strand, within BWI76_RS02195 at 455.860 kb on + strand at 455.860 kb on + strand at 455.868 kb on - strand at 455.904 kb on + strand at 456.015 kb on - strand, within BWI76_RS02200 at 456.016 kb on + strand, within BWI76_RS02200 at 456.268 kb on - strand, within BWI76_RS02200 at 456.309 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.326 kb on - strand, within BWI76_RS02200 at 456.439 kb on + strand, within BWI76_RS02200 at 456.648 kb on - strand at 456.716 kb on + strand at 456.724 kb on - strand at 456.747 kb on + strand at 456.795 kb on + strand at 456.795 kb on + strand at 456.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 453,803 - BWI76_RS02185 0.52 -0.6 453,803 - BWI76_RS02185 0.52 +0.1 453,830 + BWI76_RS02185 0.55 +0.1 453,848 - BWI76_RS02185 0.58 +0.4 453,943 + BWI76_RS02185 0.70 -0.9 454,169 - +0.6 454,274 + BWI76_RS02190 0.16 -1.4 454,343 + BWI76_RS02190 0.29 +0.0 454,559 + BWI76_RS02190 0.67 +0.9 454,695 + -0.2 454,695 + +0.3 454,695 + -0.3 454,695 + +1.1 454,763 - -1.7 454,797 + +0.5 454,920 - +1.0 454,931 + BWI76_RS02195 0.16 +0.8 455,083 - BWI76_RS02195 0.30 +1.2 455,091 - BWI76_RS02195 0.30 +0.6 455,298 - BWI76_RS02195 0.49 +0.4 455,367 + BWI76_RS02195 0.55 +0.0 455,479 + BWI76_RS02195 0.65 +0.0 455,618 - BWI76_RS02195 0.77 +0.3 455,656 - BWI76_RS02195 0.80 +3.6 455,667 + BWI76_RS02195 0.81 +1.3 455,680 - BWI76_RS02195 0.82 +0.6 455,860 + +0.1 455,860 + +0.0 455,868 - -0.2 455,904 + +0.4 456,015 - BWI76_RS02200 0.20 +0.2 456,016 + BWI76_RS02200 0.20 +0.2 456,268 - BWI76_RS02200 0.57 -0.4 456,309 - BWI76_RS02200 0.63 +0.3 456,316 - BWI76_RS02200 0.64 -2.4 456,316 - BWI76_RS02200 0.64 -0.2 456,326 - BWI76_RS02200 0.65 -1.4 456,439 + BWI76_RS02200 0.82 +1.0 456,648 - +0.1 456,716 + +1.2 456,724 - -1.0 456,747 + -0.5 456,795 + +0.8 456,795 + +0.3 456,803 - +0.5
Or see this region's nucleotide sequence