Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01190

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01185 and BWI76_RS01190 are separated by 80 nucleotidesBWI76_RS01190 and BWI76_RS01195 are separated by 138 nucleotidesBWI76_RS01195 and BWI76_RS01200 are separated by 25 nucleotides BWI76_RS01185: BWI76_RS01185 - adenylate cyclase, at 242,042 to 244,591 _RS01185 BWI76_RS01190: BWI76_RS01190 - iron donor protein CyaY, at 244,672 to 244,992 _RS01190 BWI76_RS01195: BWI76_RS01195 - membrane protein, at 245,131 to 245,334 _RS01195 BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184 _RS01200 Position (kb) 244 245Strain fitness (log2 ratio) -1 0 1at 243.956 kb on - strand, within BWI76_RS01185at 243.956 kb on - strand, within BWI76_RS01185at 244.498 kb on + strandat 244.555 kb on - strandat 244.608 kb on + strandat 244.876 kb on - strand, within BWI76_RS01190at 244.920 kb on - strand, within BWI76_RS01190at 245.186 kb on + strand, within BWI76_RS01195at 245.194 kb on - strand, within BWI76_RS01195at 245.337 kb on + strandat 245.809 kb on - strand, within BWI76_RS01200at 245.809 kb on - strand, within BWI76_RS01200at 245.838 kb on + strand, within BWI76_RS01200at 245.838 kb on + strand, within BWI76_RS01200

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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243,956 - BWI76_RS01185 0.75 +0.1
243,956 - BWI76_RS01185 0.75 -0.9
244,498 + +0.2
244,555 - -1.2
244,608 + +0.3
244,876 - BWI76_RS01190 0.64 +1.6
244,920 - BWI76_RS01190 0.77 +0.5
245,186 + BWI76_RS01195 0.27 -0.3
245,194 - BWI76_RS01195 0.31 +1.1
245,337 + +0.6
245,809 - BWI76_RS01200 0.54 +1.1
245,809 - BWI76_RS01200 0.54 -0.5
245,838 + BWI76_RS01200 0.58 +0.1
245,838 + BWI76_RS01200 0.58 +1.1

Or see this region's nucleotide sequence