Experiment: CDM1Gut with 50% spent media from Bifidobacterium adolescentis L2-32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11195 and BBR_RS11200 are separated by 151 nucleotides BBR_RS11200 and BBR_RS11205 are separated by 123 nucleotides BBR_RS11205 and BBR_RS11210 are separated by 257 nucleotides
BBR_RS11195: BBR_RS11195 - DNA polymerase III subunit delta', at 256,662 to 257,813
_RS11195
BBR_RS11200: BBR_RS11200 - LacI family transcriptional regulator, at 257,965 to 258,594
_RS11200
BBR_RS11205: BBR_RS11205 - HPr family phosphocarrier protein, at 258,718 to 258,978
_RS11205
BBR_RS11210: BBR_RS11210 - phosphoenolpyruvate--protein phosphotransferase, at 259,236 to 260,885
_RS11210
Position (kb) 258
259 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 257.821 kb on - strand at 257.822 kb on - strand at 257.822 kb on - strand at 257.823 kb on + strand at 257.843 kb on + strand at 257.855 kb on + strand at 257.855 kb on + strand at 257.855 kb on + strand at 257.855 kb on + strand at 257.861 kb on - strand at 257.863 kb on - strand at 257.878 kb on + strand at 257.886 kb on - strand at 257.886 kb on - strand at 257.886 kb on - strand at 257.902 kb on + strand at 257.902 kb on + strand at 257.902 kb on + strand at 257.902 kb on + strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.910 kb on - strand at 257.912 kb on + strand at 257.912 kb on + strand at 257.917 kb on + strand at 257.917 kb on + strand at 257.917 kb on + strand at 257.941 kb on - strand at 257.951 kb on - strand at 257.951 kb on - strand at 257.961 kb on - strand at 257.990 kb on - strand at 258.026 kb on + strand at 258.026 kb on + strand at 258.031 kb on + strand, within BBR_RS11200 at 258.056 kb on - strand, within BBR_RS11200 at 258.076 kb on - strand, within BBR_RS11200 at 258.076 kb on - strand, within BBR_RS11200 at 258.083 kb on + strand, within BBR_RS11200 at 258.088 kb on + strand, within BBR_RS11200 at 258.091 kb on - strand, within BBR_RS11200 at 258.091 kb on - strand, within BBR_RS11200 at 258.096 kb on - strand, within BBR_RS11200 at 258.118 kb on - strand, within BBR_RS11200 at 258.118 kb on - strand, within BBR_RS11200 at 258.121 kb on - strand, within BBR_RS11200 at 258.128 kb on + strand, within BBR_RS11200 at 258.128 kb on + strand, within BBR_RS11200 at 258.141 kb on - strand, within BBR_RS11200 at 258.151 kb on + strand, within BBR_RS11200 at 258.159 kb on - strand, within BBR_RS11200 at 258.165 kb on + strand, within BBR_RS11200 at 258.165 kb on + strand, within BBR_RS11200 at 258.178 kb on + strand, within BBR_RS11200 at 258.178 kb on + strand, within BBR_RS11200 at 258.178 kb on + strand, within BBR_RS11200 at 258.183 kb on + strand, within BBR_RS11200 at 258.186 kb on - strand, within BBR_RS11200 at 258.186 kb on - strand, within BBR_RS11200 at 258.213 kb on + strand, within BBR_RS11200 at 258.216 kb on + strand, within BBR_RS11200 at 258.216 kb on + strand, within BBR_RS11200 at 258.224 kb on - strand, within BBR_RS11200 at 258.235 kb on + strand, within BBR_RS11200 at 258.243 kb on - strand, within BBR_RS11200 at 258.261 kb on + strand, within BBR_RS11200 at 258.273 kb on + strand, within BBR_RS11200 at 258.294 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.359 kb on - strand, within BBR_RS11200 at 258.377 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.397 kb on + strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.440 kb on - strand, within BBR_RS11200 at 258.465 kb on + strand, within BBR_RS11200 at 258.503 kb on + strand, within BBR_RS11200 at 258.508 kb on + strand, within BBR_RS11200 at 258.516 kb on - strand, within BBR_RS11200 at 258.516 kb on - strand, within BBR_RS11200 at 258.540 kb on + strand at 258.553 kb on + strand at 258.571 kb on - strand at 258.571 kb on - strand at 258.598 kb on - strand at 258.653 kb on + strand at 258.745 kb on - strand, within BBR_RS11205 at 258.797 kb on + strand, within BBR_RS11205 at 258.797 kb on + strand, within BBR_RS11205 at 258.805 kb on - strand, within BBR_RS11205 at 258.805 kb on - strand, within BBR_RS11205 at 258.912 kb on - strand, within BBR_RS11205 at 258.961 kb on - strand at 258.983 kb on - strand at 258.983 kb on - strand at 258.997 kb on - strand at 259.045 kb on - strand at 259.045 kb on - strand at 259.045 kb on - strand at 259.046 kb on + strand at 259.052 kb on - strand at 259.087 kb on + strand at 259.101 kb on - strand at 259.162 kb on + strand at 259.170 kb on + strand at 259.170 kb on + strand at 259.183 kb on + strand at 259.211 kb on - strand at 259.213 kb on - strand at 259.214 kb on - strand at 259.214 kb on - strand at 259.231 kb on + strand at 259.347 kb on - strand at 259.481 kb on - strand, within BBR_RS11210 at 259.523 kb on + strand, within BBR_RS11210 at 259.567 kb on - strand, within BBR_RS11210 at 259.575 kb on + strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.657 kb on - strand, within BBR_RS11210 at 259.667 kb on - strand, within BBR_RS11210 at 259.669 kb on - strand, within BBR_RS11210 at 259.767 kb on + strand, within BBR_RS11210 at 259.768 kb on - strand, within BBR_RS11210 at 259.768 kb on - strand, within BBR_RS11210 at 259.869 kb on + strand, within BBR_RS11210 at 259.874 kb on + strand, within BBR_RS11210 at 259.874 kb on + strand, within BBR_RS11210 at 259.890 kb on - strand, within BBR_RS11210 at 259.897 kb on - strand, within BBR_RS11210
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1Gut with 50% spent media from Bifidobacterium adolescentis L2-32 remove 257,821 - -0.7 257,822 - +0.2 257,822 - +1.1 257,823 + -0.3 257,843 + +0.1 257,855 + -0.4 257,855 + -0.2 257,855 + -0.6 257,855 + -0.1 257,861 - +0.5 257,863 - -1.9 257,878 + +0.0 257,886 - +0.1 257,886 - +0.5 257,886 - -0.0 257,902 + +0.5 257,902 + +0.7 257,902 + +0.7 257,902 + +2.1 257,910 - +0.5 257,910 - +0.1 257,910 - -0.2 257,910 - -0.4 257,910 - +0.2 257,910 - -1.0 257,910 - -0.1 257,910 - +0.2 257,910 - -0.3 257,910 - +0.1 257,910 - +0.2 257,912 + -0.1 257,912 + +0.2 257,917 + -0.1 257,917 + +0.4 257,917 + +0.2 257,941 - +0.2 257,951 - -0.9 257,951 - -0.1 257,961 - +1.2 257,990 - +0.2 258,026 + +0.7 258,026 + +1.6 258,031 + BBR_RS11200 0.10 +1.1 258,056 - BBR_RS11200 0.14 +0.7 258,076 - BBR_RS11200 0.18 +1.1 258,076 - BBR_RS11200 0.18 +0.3 258,083 + BBR_RS11200 0.19 -0.1 258,088 + BBR_RS11200 0.20 -1.3 258,091 - BBR_RS11200 0.20 +0.5 258,091 - BBR_RS11200 0.20 +0.2 258,096 - BBR_RS11200 0.21 +2.1 258,118 - BBR_RS11200 0.24 +0.2 258,118 - BBR_RS11200 0.24 +1.1 258,121 - BBR_RS11200 0.25 -0.4 258,128 + BBR_RS11200 0.26 -0.1 258,128 + BBR_RS11200 0.26 -0.4 258,141 - BBR_RS11200 0.28 -0.9 258,151 + BBR_RS11200 0.30 +0.2 258,159 - BBR_RS11200 0.31 -0.2 258,165 + BBR_RS11200 0.32 +0.3 258,165 + BBR_RS11200 0.32 +0.3 258,178 + BBR_RS11200 0.34 +0.5 258,178 + BBR_RS11200 0.34 +0.3 258,178 + BBR_RS11200 0.34 -0.4 258,183 + BBR_RS11200 0.35 +0.3 258,186 - BBR_RS11200 0.35 +0.3 258,186 - BBR_RS11200 0.35 +0.1 258,213 + BBR_RS11200 0.39 +0.4 258,216 + BBR_RS11200 0.40 +1.1 258,216 + BBR_RS11200 0.40 -0.4 258,224 - BBR_RS11200 0.41 +1.5 258,235 + BBR_RS11200 0.43 -0.0 258,243 - BBR_RS11200 0.44 +0.5 258,261 + BBR_RS11200 0.47 +1.2 258,273 + BBR_RS11200 0.49 +0.3 258,294 + BBR_RS11200 0.52 -0.3 258,318 + BBR_RS11200 0.56 -0.4 258,318 + BBR_RS11200 0.56 -0.0 258,318 + BBR_RS11200 0.56 +0.5 258,318 + BBR_RS11200 0.56 -0.4 258,318 + BBR_RS11200 0.56 -0.4 258,318 + BBR_RS11200 0.56 -0.7 258,326 - BBR_RS11200 0.57 -0.2 258,326 - BBR_RS11200 0.57 +0.2 258,326 - BBR_RS11200 0.57 -0.4 258,326 - BBR_RS11200 0.57 +0.1 258,326 - BBR_RS11200 0.57 +1.6 258,359 - BBR_RS11200 0.63 +0.1 258,377 - BBR_RS11200 0.65 -0.4 258,391 - BBR_RS11200 0.68 -0.4 258,391 - BBR_RS11200 0.68 +0.3 258,391 - BBR_RS11200 0.68 -1.3 258,391 - BBR_RS11200 0.68 +1.3 258,397 + BBR_RS11200 0.69 -0.5 258,403 - BBR_RS11200 0.70 -0.5 258,403 - BBR_RS11200 0.70 -0.2 258,403 - BBR_RS11200 0.70 +1.1 258,403 - BBR_RS11200 0.70 -1.4 258,403 - BBR_RS11200 0.70 -1.6 258,403 - BBR_RS11200 0.70 +0.0 258,432 + BBR_RS11200 0.74 -0.3 258,432 + BBR_RS11200 0.74 -0.0 258,432 + BBR_RS11200 0.74 +0.6 258,440 - BBR_RS11200 0.75 -0.2 258,465 + BBR_RS11200 0.79 -0.1 258,503 + BBR_RS11200 0.85 -0.5 258,508 + BBR_RS11200 0.86 -0.0 258,516 - BBR_RS11200 0.87 -0.5 258,516 - BBR_RS11200 0.87 -0.5 258,540 + +0.2 258,553 + -0.2 258,571 - +0.5 258,571 - -0.2 258,598 - -0.8 258,653 + +0.7 258,745 - BBR_RS11205 0.10 -0.5 258,797 + BBR_RS11205 0.30 +1.1 258,797 + BBR_RS11205 0.30 -0.5 258,805 - BBR_RS11205 0.33 -0.7 258,805 - BBR_RS11205 0.33 -1.4 258,912 - BBR_RS11205 0.74 -1.9 258,961 - -2.4 258,983 - -3.0 258,983 - -1.1 258,997 - +0.8 259,045 - +0.5 259,045 - +1.0 259,045 - +0.7 259,046 + -0.5 259,052 - +0.3 259,087 + +0.9 259,101 - +0.4 259,162 + +0.6 259,170 + +0.7 259,170 + +0.6 259,183 + +1.5 259,211 - +0.6 259,213 - +1.3 259,214 - -2.9 259,214 - -0.9 259,231 + -1.7 259,347 - +1.1 259,481 - BBR_RS11210 0.15 -0.9 259,523 + BBR_RS11210 0.17 -2.6 259,567 - BBR_RS11210 0.20 -2.7 259,575 + BBR_RS11210 0.21 -1.3 259,644 - BBR_RS11210 0.25 -0.5 259,644 - BBR_RS11210 0.25 -0.7 259,644 - BBR_RS11210 0.25 -1.5 259,657 - BBR_RS11210 0.26 -0.1 259,667 - BBR_RS11210 0.26 -1.9 259,669 - BBR_RS11210 0.26 -0.9 259,767 + BBR_RS11210 0.32 -0.9 259,768 - BBR_RS11210 0.32 +1.1 259,768 - BBR_RS11210 0.32 -2.8 259,869 + BBR_RS11210 0.38 -3.4 259,874 + BBR_RS11210 0.39 -1.3 259,874 + BBR_RS11210 0.39 -3.5 259,890 - BBR_RS11210 0.40 -1.1 259,897 - BBR_RS11210 0.40 -1.9
Or see this region's nucleotide sequence