Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_665 and Pf6N2E2_666 are separated by 36 nucleotides Pf6N2E2_666 and Pf6N2E2_667 overlap by 1 nucleotides
Pf6N2E2_665 - Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-), at 770,028 to 771,005
_665
Pf6N2E2_666 - Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-), at 771,042 to 772,058
_666
Pf6N2E2_667 - Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-), at 772,058 to 773,170
_667
Position (kb) 771
772
773 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 770.155 kb on - strand, within Pf6N2E2_665 at 770.155 kb on - strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.290 kb on - strand, within Pf6N2E2_665 at 770.560 kb on - strand, within Pf6N2E2_665 at 770.934 kb on + strand at 770.988 kb on + strand at 770.989 kb on - strand at 770.998 kb on - strand at 771.028 kb on + strand at 771.028 kb on + strand at 771.029 kb on - strand at 771.029 kb on - strand at 771.378 kb on + strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.526 kb on - strand, within Pf6N2E2_666 at 771.601 kb on - strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.776 kb on - strand, within Pf6N2E2_666 at 771.884 kb on + strand, within Pf6N2E2_666 at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 772.072 kb on + strand at 772.782 kb on - strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.948 kb on - strand, within Pf6N2E2_667 at 772.989 kb on + strand, within Pf6N2E2_667 at 772.990 kb on - strand, within Pf6N2E2_667
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 770,155 - Pf6N2E2_665 0.13 +0.2 770,155 - Pf6N2E2_665 0.13 +0.3 770,289 + Pf6N2E2_665 0.27 +3.9 770,289 + Pf6N2E2_665 0.27 +0.3 770,290 - Pf6N2E2_665 0.27 +0.7 770,560 - Pf6N2E2_665 0.54 +0.4 770,934 + -3.0 770,988 + +0.3 770,989 - +0.4 770,998 - +0.2 771,028 + -0.5 771,028 + -0.8 771,029 - +2.2 771,029 - +2.0 771,378 + Pf6N2E2_666 0.33 -1.2 771,379 - Pf6N2E2_666 0.33 -1.7 771,379 - Pf6N2E2_666 0.33 +0.2 771,506 - Pf6N2E2_666 0.46 -0.6 771,506 - Pf6N2E2_666 0.46 +0.7 771,526 - Pf6N2E2_666 0.48 -0.6 771,601 - Pf6N2E2_666 0.55 -0.9 771,633 + Pf6N2E2_666 0.58 -1.0 771,633 + Pf6N2E2_666 0.58 -0.1 771,776 - Pf6N2E2_666 0.72 -0.9 771,884 + Pf6N2E2_666 0.83 -0.9 771,993 + +1.0 771,993 + +0.7 771,993 + +1.3 771,993 + -0.0 771,993 + -0.1 771,994 - +2.8 771,994 - +2.0 771,994 - -0.7 771,994 - +0.3 771,994 - +0.3 772,072 + +0.2 772,782 - Pf6N2E2_667 0.65 +1.1 772,895 + Pf6N2E2_667 0.75 +0.4 772,895 + Pf6N2E2_667 0.75 +0.3 772,896 - Pf6N2E2_667 0.75 -0.6 772,896 - Pf6N2E2_667 0.75 -1.6 772,948 - Pf6N2E2_667 0.80 -1.8 772,989 + Pf6N2E2_667 0.84 +0.9 772,990 - Pf6N2E2_667 0.84 -0.3
Or see this region's nucleotide sequence