Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4795

Experiment: Nickel (II) chloride 1.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4794 and Pf6N2E2_4795 are separated by 8 nucleotidesPf6N2E2_4795 and Pf6N2E2_4796 are separated by 83 nucleotidesPf6N2E2_4796 and Pf6N2E2_4797 overlap by 10 nucleotides Pf6N2E2_4794 - TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 5,543,816 to 5,544,841 _4794 Pf6N2E2_4795 - Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY, at 5,544,850 to 5,545,419 _4795 Pf6N2E2_4796 - Dihydroneopterin aldolase (EC 4.1.2.25), at 5,545,503 to 5,545,847 _4796 Pf6N2E2_4797 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 5,545,838 to 5,546,356 _4797 Position (kb) 5544 5545 5546Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 5544.851 kb on + strandat 5544.886 kb on + strandat 5544.886 kb on + strandat 5544.886 kb on + strandat 5544.887 kb on - strandat 5544.938 kb on - strand, within Pf6N2E2_4795at 5545.025 kb on + strand, within Pf6N2E2_4795at 5545.025 kb on + strand, within Pf6N2E2_4795at 5545.026 kb on - strand, within Pf6N2E2_4795at 5545.133 kb on + strand, within Pf6N2E2_4795at 5545.134 kb on - strand, within Pf6N2E2_4795at 5545.144 kb on + strand, within Pf6N2E2_4795at 5545.144 kb on + strand, within Pf6N2E2_4795at 5545.144 kb on + strand, within Pf6N2E2_4795at 5545.145 kb on - strand, within Pf6N2E2_4795at 5545.145 kb on - strand, within Pf6N2E2_4795at 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.423 kb on - strandat 5545.426 kb on + strandat 5545.452 kb on + strandat 5545.452 kb on + strandat 5546.129 kb on + strand, within Pf6N2E2_4797at 5546.130 kb on - strand, within Pf6N2E2_4797at 5546.335 kb on + strandat 5546.335 kb on + strandat 5546.335 kb on + strandat 5546.335 kb on + strandat 5546.399 kb on - strandat 5546.399 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM
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5,544,851 + +3.0
5,544,886 + +3.6
5,544,886 + +3.7
5,544,886 + +3.5
5,544,887 - -2.3
5,544,938 - Pf6N2E2_4795 0.15 -1.4
5,545,025 + Pf6N2E2_4795 0.31 +3.7
5,545,025 + Pf6N2E2_4795 0.31 +3.6
5,545,026 - Pf6N2E2_4795 0.31 -0.5
5,545,133 + Pf6N2E2_4795 0.50 +5.5
5,545,134 - Pf6N2E2_4795 0.50 -0.1
5,545,144 + Pf6N2E2_4795 0.52 +3.5
5,545,144 + Pf6N2E2_4795 0.52 +4.4
5,545,144 + Pf6N2E2_4795 0.52 +4.3
5,545,145 - Pf6N2E2_4795 0.52 -1.5
5,545,145 - Pf6N2E2_4795 0.52 -0.5
5,545,422 + +4.2
5,545,422 + +4.1
5,545,422 + +4.2
5,545,422 + +4.6
5,545,422 + +4.9
5,545,423 - +1.0
5,545,426 + +4.8
5,545,452 + +4.1
5,545,452 + +3.6
5,546,129 + Pf6N2E2_4797 0.56 -1.5
5,546,130 - Pf6N2E2_4797 0.56 -3.0
5,546,335 + -0.2
5,546,335 + -0.5
5,546,335 + -1.2
5,546,335 + +0.0
5,546,399 - -1.1
5,546,399 - -0.8

Or see this region's nucleotide sequence