Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_369

Experiment: Nickel (II) chloride 1.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_368 and Pf6N2E2_369 are separated by 40 nucleotidesPf6N2E2_369 and Pf6N2E2_370 are separated by 131 nucleotidesPf6N2E2_370 and Pf6N2E2_371 are separated by 41 nucleotides Pf6N2E2_368 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 435,040 to 436,497 _368 Pf6N2E2_369 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 436,538 to 437,617 _369 Pf6N2E2_370 - Predicted enzyme of the cupin superfamily, at 437,749 to 438,456 _370 Pf6N2E2_371 - Histone acetyltransferase HPA2 and related acetyltransferases, at 438,498 to 439,355 _371 Position (kb) 436 437 438Strain fitness (log2 ratio) -2 -1 0 1 2at 435.952 kb on + strand, within Pf6N2E2_368at 435.952 kb on + strand, within Pf6N2E2_368at 435.952 kb on + strand, within Pf6N2E2_368at 435.953 kb on - strand, within Pf6N2E2_368at 436.236 kb on + strand, within Pf6N2E2_368at 436.239 kb on + strand, within Pf6N2E2_368at 436.295 kb on - strand, within Pf6N2E2_368at 436.297 kb on + strand, within Pf6N2E2_368at 436.297 kb on + strand, within Pf6N2E2_368at 436.444 kb on + strandat 436.445 kb on - strandat 436.445 kb on - strandat 436.445 kb on - strandat 436.459 kb on + strandat 436.686 kb on - strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.689 kb on - strand, within Pf6N2E2_369at 436.754 kb on + strand, within Pf6N2E2_369at 436.844 kb on + strand, within Pf6N2E2_369at 436.844 kb on + strand, within Pf6N2E2_369at 436.845 kb on - strand, within Pf6N2E2_369at 436.955 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.406 kb on - strand, within Pf6N2E2_369at 437.490 kb on - strand, within Pf6N2E2_369at 437.740 kb on + strandat 437.751 kb on - strandat 437.763 kb on - strandat 437.763 kb on - strandat 437.777 kb on + strandat 437.777 kb on + strandat 437.777 kb on + strandat 437.777 kb on + strandat 437.777 kb on + strandat 437.778 kb on - strandat 437.821 kb on + strand, within Pf6N2E2_370at 437.869 kb on + strand, within Pf6N2E2_370at 437.869 kb on + strand, within Pf6N2E2_370at 437.937 kb on - strand, within Pf6N2E2_370at 437.937 kb on - strand, within Pf6N2E2_370at 437.956 kb on - strand, within Pf6N2E2_370at 437.956 kb on - strand, within Pf6N2E2_370at 438.238 kb on + strand, within Pf6N2E2_370at 438.345 kb on - strand, within Pf6N2E2_370

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM
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435,952 + Pf6N2E2_368 0.63 +1.0
435,952 + Pf6N2E2_368 0.63 -1.5
435,952 + Pf6N2E2_368 0.63 -0.1
435,953 - Pf6N2E2_368 0.63 -0.6
436,236 + Pf6N2E2_368 0.82 +0.4
436,239 + Pf6N2E2_368 0.82 -0.6
436,295 - Pf6N2E2_368 0.86 +0.5
436,297 + Pf6N2E2_368 0.86 +0.2
436,297 + Pf6N2E2_368 0.86 -0.7
436,444 + -0.3
436,445 - -0.5
436,445 - -1.3
436,445 - -0.5
436,459 + -0.1
436,686 - Pf6N2E2_369 0.14 +0.8
436,688 + Pf6N2E2_369 0.14 +0.8
436,688 + Pf6N2E2_369 0.14 +1.3
436,688 + Pf6N2E2_369 0.14 +1.5
436,688 + Pf6N2E2_369 0.14 +0.9
436,688 + Pf6N2E2_369 0.14 +0.5
436,688 + Pf6N2E2_369 0.14 +0.5
436,689 - Pf6N2E2_369 0.14 -0.1
436,754 + Pf6N2E2_369 0.20 +0.5
436,844 + Pf6N2E2_369 0.28 -1.5
436,844 + Pf6N2E2_369 0.28 -0.3
436,845 - Pf6N2E2_369 0.28 -0.7
436,955 + Pf6N2E2_369 0.39 +0.0
437,405 + Pf6N2E2_369 0.80 +0.5
437,405 + Pf6N2E2_369 0.80 +1.4
437,405 + Pf6N2E2_369 0.80 +0.8
437,405 + Pf6N2E2_369 0.80 +0.2
437,406 - Pf6N2E2_369 0.80 +0.9
437,490 - Pf6N2E2_369 0.88 +0.2
437,740 + -0.1
437,751 - -0.9
437,763 - -1.1
437,763 - +0.3
437,777 + -0.5
437,777 + +0.3
437,777 + -0.2
437,777 + +0.3
437,777 + +0.1
437,778 - -0.6
437,821 + Pf6N2E2_370 0.10 +0.7
437,869 + Pf6N2E2_370 0.17 -1.5
437,869 + Pf6N2E2_370 0.17 +0.5
437,937 - Pf6N2E2_370 0.27 -1.9
437,937 - Pf6N2E2_370 0.27 -0.3
437,956 - Pf6N2E2_370 0.29 +1.2
437,956 - Pf6N2E2_370 0.29 -0.2
438,238 + Pf6N2E2_370 0.69 -0.5
438,345 - Pf6N2E2_370 0.84 +2.5

Or see this region's nucleotide sequence