Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26500 and BWI76_RS26505 overlap by 1 nucleotides BWI76_RS26505 and BWI76_RS26510 overlap by 4 nucleotides BWI76_RS26510 and BWI76_RS26515 overlap by 1 nucleotides
BWI76_RS26500: BWI76_RS26500 - nickel ABC transporter, nickel/metallophore periplasmic binding protein, at 5,484,975 to 5,486,543
_RS26500
BWI76_RS26505: BWI76_RS26505 - nickel ABC transporter permease subunit NikB, at 5,486,543 to 5,487,487
_RS26505
BWI76_RS26510: BWI76_RS26510 - nickel ABC transporter permease subunit NikC, at 5,487,484 to 5,488,317
_RS26510
BWI76_RS26515: BWI76_RS26515 - nickel import ATP-binding protein NikD, at 5,488,317 to 5,489,078
_RS26515
Position (kb) 5486
5487
5488 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5485.638 kb on - strand, within BWI76_RS26500 at 5485.643 kb on - strand, within BWI76_RS26500 at 5485.711 kb on - strand, within BWI76_RS26500 at 5485.796 kb on - strand, within BWI76_RS26500 at 5485.836 kb on + strand, within BWI76_RS26500 at 5485.839 kb on - strand, within BWI76_RS26500 at 5485.886 kb on - strand, within BWI76_RS26500 at 5486.002 kb on + strand, within BWI76_RS26500 at 5486.303 kb on + strand, within BWI76_RS26500 at 5486.464 kb on + strand at 5486.472 kb on - strand at 5486.493 kb on - strand at 5486.522 kb on + strand at 5486.583 kb on - strand at 5487.167 kb on + strand, within BWI76_RS26505 at 5487.213 kb on + strand, within BWI76_RS26505 at 5487.226 kb on - strand, within BWI76_RS26505 at 5487.305 kb on + strand, within BWI76_RS26505 at 5487.386 kb on - strand, within BWI76_RS26505 at 5487.448 kb on + strand at 5487.663 kb on - strand, within BWI76_RS26510 at 5487.821 kb on + strand, within BWI76_RS26510 at 5487.855 kb on + strand, within BWI76_RS26510 at 5487.880 kb on + strand, within BWI76_RS26510 at 5487.883 kb on - strand, within BWI76_RS26510 at 5487.974 kb on + strand, within BWI76_RS26510 at 5488.014 kb on - strand, within BWI76_RS26510 at 5488.040 kb on + strand, within BWI76_RS26510 at 5488.194 kb on - strand, within BWI76_RS26510 at 5488.222 kb on - strand, within BWI76_RS26510 at 5488.246 kb on + strand at 5488.355 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,485,638 - BWI76_RS26500 0.42 +0.6 5,485,643 - BWI76_RS26500 0.43 +0.9 5,485,711 - BWI76_RS26500 0.47 +0.5 5,485,796 - BWI76_RS26500 0.52 +1.2 5,485,836 + BWI76_RS26500 0.55 +1.9 5,485,839 - BWI76_RS26500 0.55 -0.2 5,485,886 - BWI76_RS26500 0.58 +1.1 5,486,002 + BWI76_RS26500 0.65 +0.2 5,486,303 + BWI76_RS26500 0.85 +1.7 5,486,464 + -2.6 5,486,472 - +1.1 5,486,493 - +1.2 5,486,522 + -0.2 5,486,583 - -0.2 5,487,167 + BWI76_RS26505 0.66 +0.7 5,487,213 + BWI76_RS26505 0.71 +0.2 5,487,226 - BWI76_RS26505 0.72 -2.0 5,487,305 + BWI76_RS26505 0.81 -3.1 5,487,386 - BWI76_RS26505 0.89 -0.7 5,487,448 + +0.0 5,487,663 - BWI76_RS26510 0.21 +0.5 5,487,821 + BWI76_RS26510 0.40 -0.6 5,487,855 + BWI76_RS26510 0.44 +0.3 5,487,880 + BWI76_RS26510 0.47 +0.7 5,487,883 - BWI76_RS26510 0.48 +0.4 5,487,974 + BWI76_RS26510 0.59 +1.0 5,488,014 - BWI76_RS26510 0.64 +0.2 5,488,040 + BWI76_RS26510 0.67 -0.9 5,488,194 - BWI76_RS26510 0.85 +0.2 5,488,222 - BWI76_RS26510 0.88 +1.0 5,488,246 + -1.5 5,488,355 + -0.8
Or see this region's nucleotide sequence