Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26490 and BWI76_RS26495 are separated by 100 nucleotides BWI76_RS26495 and BWI76_RS26500 are separated by 374 nucleotides
BWI76_RS26490: BWI76_RS26490 - phenolic acid decarboxylase, at 5,483,136 to 5,483,639
_RS26490
BWI76_RS26495: BWI76_RS26495 - LysR family transcriptional regulator, at 5,483,740 to 5,484,600
_RS26495
BWI76_RS26500: BWI76_RS26500 - nickel ABC transporter, nickel/metallophore periplasmic binding protein, at 5,484,975 to 5,486,543
_RS26500
Position (kb) 5483
5484
5485 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5482.749 kb on + strand at 5482.749 kb on + strand at 5482.749 kb on + strand at 5482.757 kb on - strand at 5482.757 kb on - strand at 5482.786 kb on + strand at 5482.800 kb on + strand at 5482.803 kb on - strand at 5482.803 kb on - strand at 5482.810 kb on - strand at 5482.831 kb on - strand at 5482.885 kb on + strand at 5482.885 kb on + strand at 5482.885 kb on + strand at 5482.885 kb on + strand at 5482.895 kb on + strand at 5482.895 kb on + strand at 5482.895 kb on + strand at 5482.903 kb on - strand at 5482.995 kb on + strand at 5482.995 kb on - strand at 5482.995 kb on - strand at 5482.995 kb on - strand at 5483.035 kb on + strand at 5483.134 kb on + strand at 5483.134 kb on + strand at 5483.135 kb on + strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.174 kb on - strand at 5483.181 kb on + strand at 5483.188 kb on + strand, within BWI76_RS26490 at 5483.193 kb on + strand, within BWI76_RS26490 at 5483.201 kb on - strand, within BWI76_RS26490 at 5483.201 kb on - strand, within BWI76_RS26490 at 5483.206 kb on + strand, within BWI76_RS26490 at 5483.223 kb on + strand, within BWI76_RS26490 at 5483.230 kb on - strand, within BWI76_RS26490 at 5483.270 kb on - strand, within BWI76_RS26490 at 5483.332 kb on + strand, within BWI76_RS26490 at 5483.408 kb on + strand, within BWI76_RS26490 at 5483.421 kb on - strand, within BWI76_RS26490 at 5483.465 kb on + strand at 5483.467 kb on + strand, within BWI76_RS26490 at 5483.473 kb on - strand at 5483.475 kb on - strand, within BWI76_RS26490 at 5483.525 kb on + strand, within BWI76_RS26490 at 5483.526 kb on + strand, within BWI76_RS26490 at 5483.594 kb on - strand at 5483.615 kb on + strand at 5483.623 kb on - strand at 5483.650 kb on + strand at 5483.879 kb on + strand, within BWI76_RS26495 at 5483.914 kb on + strand, within BWI76_RS26495 at 5483.914 kb on + strand, within BWI76_RS26495 at 5483.949 kb on + strand, within BWI76_RS26495 at 5483.957 kb on - strand, within BWI76_RS26495 at 5483.993 kb on + strand, within BWI76_RS26495 at 5484.001 kb on - strand, within BWI76_RS26495 at 5484.083 kb on + strand, within BWI76_RS26495 at 5484.417 kb on + strand, within BWI76_RS26495 at 5484.585 kb on + strand at 5484.585 kb on + strand at 5484.616 kb on - strand at 5484.616 kb on - strand at 5484.716 kb on + strand at 5485.097 kb on + strand at 5485.151 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.313 kb on - strand, within BWI76_RS26500 at 5485.480 kb on - strand, within BWI76_RS26500
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,482,749 + +0.0 5,482,749 + +0.2 5,482,749 + +1.4 5,482,757 - +0.1 5,482,757 - +1.1 5,482,786 + -0.5 5,482,800 + +1.2 5,482,803 - +3.2 5,482,803 - -0.4 5,482,810 - -2.0 5,482,831 - -0.3 5,482,885 + -1.7 5,482,885 + -2.9 5,482,885 + +0.1 5,482,885 + +0.1 5,482,895 + +0.3 5,482,895 + -0.4 5,482,895 + +0.6 5,482,903 - -0.8 5,482,995 + -0.2 5,482,995 - -1.4 5,482,995 - -1.4 5,482,995 - -0.2 5,483,035 + +0.1 5,483,134 + -2.0 5,483,134 + -1.8 5,483,135 + +0.6 5,483,142 - -0.1 5,483,142 - -2.0 5,483,142 - -3.0 5,483,142 - -0.6 5,483,142 - +1.0 5,483,142 - -1.8 5,483,174 - +0.6 5,483,181 + -0.0 5,483,188 + BWI76_RS26490 0.10 -0.2 5,483,193 + BWI76_RS26490 0.11 +1.2 5,483,201 - BWI76_RS26490 0.13 -0.9 5,483,201 - BWI76_RS26490 0.13 -1.2 5,483,206 + BWI76_RS26490 0.14 +0.5 5,483,223 + BWI76_RS26490 0.17 +0.2 5,483,230 - BWI76_RS26490 0.19 -1.0 5,483,270 - BWI76_RS26490 0.27 +0.3 5,483,332 + BWI76_RS26490 0.39 +0.3 5,483,408 + BWI76_RS26490 0.54 +0.2 5,483,421 - BWI76_RS26490 0.57 -0.4 5,483,465 + +0.2 5,483,467 + BWI76_RS26490 0.66 -0.4 5,483,473 - +0.2 5,483,475 - BWI76_RS26490 0.67 -1.8 5,483,525 + BWI76_RS26490 0.77 -0.4 5,483,526 + BWI76_RS26490 0.77 -0.1 5,483,594 - +0.2 5,483,615 + -0.3 5,483,623 - -0.8 5,483,650 + +0.2 5,483,879 + BWI76_RS26495 0.16 +0.4 5,483,914 + BWI76_RS26495 0.20 -0.6 5,483,914 + BWI76_RS26495 0.20 +0.6 5,483,949 + BWI76_RS26495 0.24 -0.4 5,483,957 - BWI76_RS26495 0.25 -0.9 5,483,993 + BWI76_RS26495 0.29 -1.4 5,484,001 - BWI76_RS26495 0.30 +0.1 5,484,083 + BWI76_RS26495 0.40 +1.9 5,484,417 + BWI76_RS26495 0.79 -0.1 5,484,585 + -0.8 5,484,585 + -1.6 5,484,616 - -2.0 5,484,616 - -0.5 5,484,716 + -0.4 5,485,097 + +1.0 5,485,151 + BWI76_RS26500 0.11 +0.7 5,485,225 + BWI76_RS26500 0.16 +1.2 5,485,225 + BWI76_RS26500 0.16 +1.2 5,485,225 + BWI76_RS26500 0.16 +0.2 5,485,313 - BWI76_RS26500 0.22 +0.1 5,485,480 - BWI76_RS26500 0.32 -0.8
Or see this region's nucleotide sequence